본문 바로가기
KERD
논문KCI 등재

Identification of heat shock protein70-2 and protamine-1 mRNA, proteins, and analyses of their association with fertility using frozen-thawed sperm in Madura bulls

2

조회

0

다운로드

0

인용

0

참고문헌

키워드

초록

Objective: This study aims to identify heat shock protein70-2 (HSP70-2) and protamine-1 (PRM1) mRNA and protein in Madura bull sperm and demonstrate their relation as bull fertility biomarkers. Methods: The Madura bull fertility rates were grouped based on the percentage of first service conception rate (%FSCR) as high fertility (HF) (79.04%; n = 4), and low fertility (LF) (65.84%; n = 4). mRNA of HSP70-2 and PRM1 with peptidylprolyl isomerase A (PPIA) as a housekeeping gene were determined by quantitative real-time polymerase chain reaction, while enzyme-linked immunoassay was used to measure protein abundance. In the post-thawed semen samples, sperm motility, viability, acrosome integrity, and sperm DNA fragmentation index were analyzed. Data analysis was performed on the measured parameters of semen quality, relative mRNA expression, and protein abundance of HSP70-2 and PRM1, among the bulls with various fertility levels (HF and LF) in a one-way analysis of variance analysis. The Pearson correlation was used to analyze the relationship between semen quality, mRNA, proteins, and fertility rate. Results: Relative mRNA expression and protein abundance of HSP70-2 and PRM1 were detected and were found to be highly expressed in bulls with HF (p<0.05) and were associated with several parameters of semen quality. Conclusion: HSP70-2 and PRM1 mRNA and protein molecules have great potential to serve as molecular markers for determining bull fertility.

Abstract

Objective: This study aims to identify heat shock protein70-2 (HSP70-2) and protamine-1 (PRM1) mRNA and protein in Madura bull sperm and demonstrate their relation as bull fertility biomarkers. Methods: The Madura bull fertility rates were grouped based on the percentage of first service conception rate (%FSCR) as high fertility (HF) (79.04%; n = 4), and low fertility (LF) (65.84%; n = 4). mRNA of HSP70-2 and PRM1 with peptidylprolyl isomerase A (PPIA) as a housekeeping gene were determined by quantitative real-time polymerase chain reaction, while enzyme-linked immunoassay was used to measure protein abundance. In the post-thawed semen samples, sperm motility, viability, acrosome integrity, and sperm DNA fragmentation index were analyzed. Data analysis was performed on the measured parameters of semen quality, relative mRNA expression, and protein abundance of HSP70-2 and PRM1, among the bulls with various fertility levels (HF and LF) in a one-way analysis of variance analysis. The Pearson correlation was used to analyze the relationship between semen quality, mRNA, proteins, and fertility rate. Results: Relative mRNA expression and protein abundance of HSP70-2 and PRM1 were detected and were found to be highly expressed in bulls with HF (p<0.05) and were associated with several parameters of semen quality. Conclusion: HSP70-2 and PRM1 mRNA and protein molecules have great potential to serve as molecular markers for determining bull fertility.

참고문헌 (29)

  1. Kutsiyah F, "Population dynamic and productivity of Madura cattle in corservation area of sapudi island", Sains Peternakan, 15, pp.70~77 (2017)
  2. Zuhri M, "Evaluation of the reproductive performance of Madura cattle raised by small scale farmers in Madura, Indonesia", Livest Res Rural Dev, 31, pp.70~ (2019)
  3. Diskin MG, "Review : Semen handling, time of insemination and insemination technique in cattle", Animal, 12(s1), pp.s75~ (2018)[DOI]
  4. Rosyada ZNA, "Sperm protein markers for Holstein bull fertility at national artificial insemination centers in Indonesia", Vet World, 13, pp.947~955 (2020)[DOI]
  5. Jodar M, "Sperm and seminal plasma RNAs : What roles do they play beyond fertilization?", Reproduction, 158, pp.R113~ (2019)[DOI]
  6. TANGA BEREKET MOLLA, "Semen evaluation: methodological advancements in sperm quality-specific fertility assessment — A review", Animal Bioscience, 34(8), pp.1253~1270 (2021), 아세아·태평양축산학회[DOI]
  7. Rosyada ZNA, "Implications of sperm heat shock protein 70-2 in bull fertility", Vet World, 15, pp.1456~1466 (2022)[DOI]
  8. Pardede BP, "PRM1 gene expression and its protein abundance in frozen-thawed spermatozoa as potential fertility markers in breeding bulls", Vet Sci, 9, pp.111~ (2022)[DOI]
  9. Sahoo B, "Significance and relevance of spermatozoal RNAs to male fertility in livestock", Front Genet, 12, pp.768196~ (2021)[DOI]
  10. Liu P, "Regulation by HSP70", 90 in the different tissues and testis development of male cattle (Cattleyak and Yak)
  11. Muhammad Aslam MKM, "Identification of biomarker candidates for fertility in spermatozoa of crossbred bulls through comparative proteomics", Theriogenology, 119, pp.43~51 (2018)[DOI]
  12. Sundararaman MN, "Computer assisted semen analysis for quantification of motion characteristics of bull sperm during cryopreservation cycle", Vet World, 5, pp.723~726 (2012)[DOI]
  13. Cheng F, "Use of peanut agglutinin to assess the acrosomal status and the zona pellucida-induced acrosome reaction in stallion spermatozoa", J Androl, 17, pp.674~682 (1996)
  14. Said S, "Development of rat oocytes following intracytoplasmic injection of sperm heads isolated from testicular and epididymal spermatozoa", Theriogenology, 60, pp.359~369 (2003)[DOI]
  15. Livak KJ, "Analysis of relative gene expression data using real-time quantitative PCR and the 2-ΔΔCT method", Methods, 25, pp.402~408 (2001)[DOI]
  16. Pardede BP, "Relationship of frozen thawed semen quality with the fertility rate after being distributed in the brahman cross breeding program", Vet World, 13, pp.2649~2657 (2020)[DOI]
  17. Bahmid NA, "The quality of frozen Friesian Holstein semen after long-term storage", Trop Anim Sci J, 46, pp.13~19 (2023)[DOI]
  18. Orsolini MF, "An update on semen physiology, technologies, and selection techniques for the advancement of in vitro equine embryo production : section II", Animals(Basel), 11, pp.3319~ (2021)[DOI]
  19. Pardede BP, "The potential of sperm bovine protamine as a protein marker of semen production and quality at the National Artificial Insemination Center of Indonesia", Vet World, 14, pp.2473~2481 (2021)[DOI]
  20. Butler ML, "Selection for bull fertility : A review", Transl Anim Sci, 4, pp.423~441 (2020)[DOI]
  21. Bhat S, "Effect of heat shock protein 70 polymorphism on thermotolerance in Tharparkar cattle", Vet World, 9, pp.113~117 (2016)[DOI]
  22. Feugang JM, "Transcriptome analysis of bull spermatozoa : Implications for male fertility", Reprod Biomed Online, 21, pp.312~324 (2010)[DOI]
  23. Redgrove KA, "The molecular chaperone HSPA2 plays a key role in regulating the expression of sperm surface receptors that mediate sperm-egg recognition", PLOS ONE, 7, pp.e50851~ (2012)[DOI]
  24. Somashekar L, "Comparative sperm protein profiling in bulls differing in fertility and identification of protein 4, a potential fertility marker", Andrology, 5, pp.1032~1051 (2017)[DOI]
  25. Zhang XG, "Association of heat shock protein 70 with motility of frozenthawed sperm in bulls", Czech J Anim Sci, 60, pp.256~262 (2015)[DOI]
  26. Chen X, "Comparative transcript profiling of gene expression of fresh and frozen–thawed bull sperm", Theriogenology, 83, pp.504~511 (2015)[DOI]
  27. Ramesha KP, "Deep proteome profiling of semen of Indian indigenous Malnad Gidda(Bos indicus)cattle", J Proteome Res, 19, pp.3364~3376 (2020)[DOI]
  28. Govin J, "Post-meiotic shifts in HSPA2", HSP70. 2 chaperone activity during mouse spermatogenesis, 2006, pp.281, 37888~37892
  29. Schneider S, "Re-visiting the Protamine-2 locus : Deletion, but not haploinsufficiency, renders male mice infertile", Sci Rep, 6, pp.36764~ (2016)[DOI]

관련 논문AI추천

이 간행물의 다른 논문 (14건)