Association of polymorphisms in Pit-1 gene with growth and feed efficiency in meat-type chickens
2
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
Objective: The pituitary specific transcription factor-1 (Pit-1) gene is responsible for pituitary development and growth hormone expression and is regarded as a pivotal candidate gene for growth and production in chickens. Therefore, the aim of this study was to investigate the association of Pit-1 polymorphisms with growth and feed efficiency traits in yellow meat-type chickens. Methods: In the present study, five single nucleotide polymorphisms (SNPs) of Pit-1 were selected and genotyped by high-throughput matrix-assisted laser desorption-ionization time-of-flight mass spectrometry in 724 meat-type chickens. Results: Association analysis showed that rs13687126 of Pit-1 was strongly associated with body weight gain (BWG) and feed intake (FI) (p<0.05), and that rs13687128 was significantly correlated with body weight at 70 days of age (BW70), BWG and feed conversion ratio (FCR) (p<0.05). SNP rs13905622 was strongly related to BW70 and FCR (p<0.05). Furthermore, birds with the GG genotype of rs13687126 had larger BWG and FI than those with the AG genotype (p<0.05). Individuals with the TT genotype of rs13687128 were significantly higher BW70 and BWG than those of the CT and CC genotype, while FCR was just the opposite (p<0.05). For rs13905622, the AA chickens showed strongly larger BW70 and lower FCR compared with the AT and TT chickens (p<0.05). Additionally, an ACA haplotype based on rs13687126, rs13687128, and rs13905622 had significant effects on BW70 and FCR (p<0.05). Conclusion: Our studies thus provide crucial evidence for the relationship between polymorphisms of Pit-1 and growth and feed efficiency traits which may be useful for meat-type chicken breeding programs.
Abstract
Objective: The pituitary specific transcription factor-1 (Pit-1) gene is responsible for pituitary development and growth hormone expression and is regarded as a pivotal candidate gene for growth and production in chickens. Therefore, the aim of this study was to investigate the association of Pit-1 polymorphisms with growth and feed efficiency traits in yellow meat-type chickens. Methods: In the present study, five single nucleotide polymorphisms (SNPs) of Pit-1 were selected and genotyped by high-throughput matrix-assisted laser desorption-ionization time-of-flight mass spectrometry in 724 meat-type chickens. Results: Association analysis showed that rs13687126 of Pit-1 was strongly associated with body weight gain (BWG) and feed intake (FI) (p<0.05), and that rs13687128 was significantly correlated with body weight at 70 days of age (BW70), BWG and feed conversion ratio (FCR) (p<0.05). SNP rs13905622 was strongly related to BW70 and FCR (p<0.05). Furthermore, birds with the GG genotype of rs13687126 had larger BWG and FI than those with the AG genotype (p<0.05). Individuals with the TT genotype of rs13687128 were significantly higher BW70 and BWG than those of the CT and CC genotype, while FCR was just the opposite (p<0.05). For rs13905622, the AA chickens showed strongly larger BW70 and lower FCR compared with the AT and TT chickens (p<0.05). Additionally, an ACA haplotype based on rs13687126, rs13687128, and rs13905622 had significant effects on BW70 and FCR (p<0.05). Conclusion: Our studies thus provide crucial evidence for the relationship between polymorphisms of Pit-1 and growth and feed efficiency traits which may be useful for meat-type chicken breeding programs.
참고문헌 (24)
- Patience JF, "A review of feed efficiency in swine: biology and application", J Anim Sci Biotechnol, 6, pp.33~ (2015)
- de Verdal H, "Improving the efficiency of feed utilization in poultry by selection. 2. Genetic parameters of excretion traits and correlations with anatomy of the gastro-intestinal tract and digestive efficiency", BMC Genet, 12, pp.71~ (2011)
- Steinfelder HJ, "Thyrotropin-releasing hormone regulation of human TSHB expression: role of a pituitary-specific transcription factor (Pit-1", GHF-1) and potential interaction with a thyroid hormone-inhibitory element, 1991, pp.88, 3130~3134
- Li S, "Dwarf locus mutants lacking three pituitary cell types result from mutations in the POU-domain gene pit-1", Nature, 347, pp.528~533 (1990)
- Lee EJ, "Pituitary transcription factor-1 induces transient differentiation of adult hepatic stem cells into prolactin-producing cells in vivo", Mol Endocrinol, 19, pp.964~971 (2005)
- Taha D, "Absent or delayed adrenarche in Pit-1", POU1F1 deficiency, 2005, pp.64, 175~179
- Sobrier ML, "Functional characterization of a human POU1F1 mutation associated with isolated growth hormone deficiency: a novel etiology for IGHD", Hum Mol Genet, 25, pp.472~483 (2016)
- Hoseinzadeh ZE, "Association of PIT1 gene and milk protein percentage in Holstein cattle", J Livest Sci Tech, 31, pp.40~49 (2015)
- Piórkowska K, "Association study of PIT1 and GHRH SNPs with economically important traits in pigs of three breeds reared in Poland", Anim Sci Pap Rep, 31, pp.303~314 (2013)
- Tang LG, "Association of polymorphisms in the Pit-1 intron 5 with body measurements in Chinese Cattle", Afr J Biotechnol, 11, pp.9906~9910 (2012)
- McElroy JP, "Identification of trait loci affecting white meat percentage and other growth and carcass traits in commercial broiler chickens", Poult Sci, 85, pp.593~605 (2006)
- Nie Q, "The PIT1 gene polymorphisms were associated with chicken growth traits", BMC Genet, 9, pp.20~ (2008)
- Xu HY, "Polymorphisms and expression of the chicken POU1F1 gene associated with carcass traits", Mol Biol Rep, 39, pp.8363~8371 (2012)
- Jin S, "Polymorphisms in the transforming growth factor beta3 gene and their associations with feed efficiency in chickens", Poult Sci, 92, pp.1745~1749 (2013)
- Barrett JC, "Haploview: analysis and visualization of LD and haplotype maps", Bioinformatics, 21, pp.263~265 (2005)
- Ardlie KG, "Patterns of linkage disequilibrium in the human genome", Nat Rev Genet, 3, pp.299~309 (2002)
- Bona G, "Genetic defects in GH synthesis and secretion", Eur J Endocrinol, 151(Suppl 1), pp.S3~S9 (2004)
- Cohen LE, "Role of Pit-1 in the gene expression of growth hormone, prolactin, and thyrotropin", Endocrinol Metab Clin North Am, 25, pp.523~540 (1996)
- Sewalem A, "Mapping of quantitative trait loci for body weight at three, six, and nine weeks of age in a broiler layer cross", Poult Sci, 81, pp.1775~1781 (2002)
- Prabuddha Manjula, "POU class 1 homeobox 1 gene polymorphisms associated with growth traits in Korean native chicken", Animal Bioscience, 31(5), pp.643~649 (2018), 아세아·태평양축산학회[DOI]
- Selvaggi M, "Analysis of two pit-1 gene polymorphisms and relationships with growth performance traits in Podolica young bulls", Livest Sci, 38, pp.308~312 (2011)
- Bhattacharya TK, "Polymorphisms of Pit-1 gene and its association with growth traits in chicken", Poult Sci, 91, pp.1057~1064 (2012)
- Song C, "MspI polymorphisms in the 3rd intron of the swine POU1F1 gene and their associations with growth performance", J Appl Genet, 46, pp.285~289 (2005)
- Sadeghi M, "Associations of POU1F1 gene polymorphisms and protein structure changes with growth traits and blood metabolites in two Iranian sheep breeds", pp.831~835 (2014), J Genet