Profiling of differentially expressed proteins between fresh and frozen-thawed Duroc boar semen using Protein- Chip CM10
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Many studies have been conducted to improve technology for semen cryopreservation in pigs. However, computer-assisted analysis of sperm motility and morphology is insufficient to predict the molecular function of frozen-thawed semen. More accurate expression patterns of boar sperm proteins may be derived using the isobaric tags for relative and absolute quantification (iTRAQ) technique. In this study, the iTRAQ-labeling system was coupled with liquid chromatography tandem-mass spectrometry (LC-MS/MS) analysis to identify differentially expressed CM10-fractionated proteins between fresh and frozen-thawed boar semen. A total of 76 protein types were identified to be differentially expressed, among which 9 and 67 proteins showed higher and lower expression in frozen-thawed than in fresh sperm samples, respectively. The classified functions of these proteins included oxidative phosphorylation, mitochondrial inner membrane and matrix, and pyruvate metabolic processes, which are involved in adenosine triphosphate (ATP) synthesis; and sperm flagellum and motile cilium, which are involved in sperm tail structure. These results suggest a possible network of biomarkers associated with survival after the cryopreservation of Duroc boar semen.
Abstract
Many studies have been conducted to improve technology for semen cryopreservation in pigs. However, computer-assisted analysis of sperm motility and morphology is insufficient to predict the molecular function of frozen-thawed semen. More accurate expression patterns of boar sperm proteins may be derived using the isobaric tags for relative and absolute quantification (iTRAQ) technique. In this study, the iTRAQ-labeling system was coupled with liquid chromatography tandem-mass spectrometry (LC-MS/MS) analysis to identify differentially expressed CM10-fractionated proteins between fresh and frozen-thawed boar semen. A total of 76 protein types were identified to be differentially expressed, among which 9 and 67 proteins showed higher and lower expression in frozen-thawed than in fresh sperm samples, respectively. The classified functions of these proteins included oxidative phosphorylation, mitochondrial inner membrane and matrix, and pyruvate metabolic processes, which are involved in adenosine triphosphate (ATP) synthesis; and sperm flagellum and motile cilium, which are involved in sperm tail structure. These results suggest a possible network of biomarkers associated with survival after the cryopreservation of Duroc boar semen.
참고문헌 (25)
- Sieme H, "Cryopreservation and freeze-drying protocols", pp.277~287 (2015), Springer
- Yeste M, "Sperm cryopreservation update : cryodamage, markers, and factors affecting the sperm freezability in pigs", Theriogenology, 85, pp.47~64 (2016)[DOI]
- Watson PF, "The causes of reduced fertility with cryopreserved semen", Anim Reprod Sci, 60-61, pp.481~492 (2000)[DOI]
- Rodríguez-Gil JE, "Boar reproduction: fundamentals and new biotechnological trends", pp.589~607 (2013), Springer
- Yeste M, "Recent advances in boar sperm cryopreservation : state of the art and current perspectives", Reprod Domest Anim, 50, pp.71~79 (2015)[DOI]
- Okazaki T, "New strategies of boar sperm cryopreservation : development of novel freezing and thawing methods with a focus on the roles of seminal plasma", Anim Sci J, 83, pp.623~629 (2012)[DOI]
- Okazaki T, "Artificial insemination with seminal plasma improves the reproductive performance of frozen-thawed boar epididymal spermatozoa", J Androl, 33, pp.990~998 (2012)[DOI]
- Techakumphu M, "Success in artificial insemination-quality of semen and diagnostics employed", pp.17~37 (2013), IntechOpen
- Casas I, "Boar reproduction: fundamentals and new biotechnological trends", pp.551~588 (2013), Springer
- Vadnais ML, "Characterization of capacitation, cryoinjury, and the role of seminal plasma in porcine sperm", Theriogenology, 76, pp.1508~1516 (2011)[DOI]
- Stănescu M, "Freezing of dog’s sperm : a review", Rom Biotechnol Lett, 17, pp.7709~7716 (2012)
- Jeong YJ, "Effect of α-tocopherol supplementation during boar semen cryopreservation on sperm characteristics and expression of apoptosis related genes", Cryobiology, 58, pp.181~189 (2009)[DOI]
- Wang S, "Proteomic characteristics of human sperm cryopreservation", Proteomics, 14, pp.298~310 (2014)[DOI]
- Zilli L, "Effect of cryopreservation on sea bass sperm proteins", Biol Reprod, 72, pp.1262~1267 (2005)[DOI]
- He Y, "Differential proteome association study of freeze-thaw damage in ram sperm", Cryobiology, 72, pp.60~68 (2016)[DOI]
- Cheng CY, "Differential protein expression in chicken spermatozoa before and after freezing–thawing treatment", Anim Reprod Sci, 152, pp.99~107 (2015)[DOI]
- Dietrich MA, "Proteomic analysis of extracellular medium of cryopreserved carp(Cyprinus carpio L. )semen", Comp Biochem Physiol D Genomics Proteomics, 15, pp.49~57 (2015)[DOI]
- Niu D, "iTRAQ-coupled 2-D LC-MS", MS analysis of protein profile associated with HBV-modulated DNA methylation, 2009, pp.9, 3856~3868
- Chen X, "Identification of differentially expressed proteins in fresh and frozen-thawed boar spermatozoa by iTRAQ-coupled 2D LC–MS", MS, 2014, pp.147, 321~330
- Westendorf P, "Deep freezing of boar sperma. Laboratory and insemination results using the Hülsenberger paillete method", Dtsch Tierarztl Wochenschr, 82, pp.261~267 (1975)
- Bradford MM, "A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding", Anal Biochem, 72, pp.248~254 (1976)[DOI]
- Wu WW, "Comparative study of three proteomic quantitative methods, DIGE, cICAT, and iTRAQ, using 2D gel-or LC-MALDI TOF", TOF, 2006, pp.5, 651~658
- Poon TCW, "Opportunities and limitations of SELDI-TOF-MS in biomedical research : practical advices", Expert Rev Proteomic, 4, pp.51~65 (2007)[DOI]
- De Leeuw FE, "The role membrane damage plays in cold shock and freezing injury", Reprod Domest Anim, 1, pp.95~104 (1991)
- Parks JE, "Effects of cryopreservation procedures on sperm membranes", Theriogenology, 38, pp.209~222 (1992)[DOI]