The variation of insulin like growth factor 2 maker is associated with growth traits in Thai native (Kradon) pigs
2
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
Objective: This study was conducted to investigate polymorphisms of the melanocortin-4 receptor (MC4R) and insulin like growth factor 2 (IGF2) genes and to evaluate the growth traits affected by such polymorphisms in Thai native (Kradon) pigs. Methods: Blood samples and productive data from 91 Kradon pigs were collected. DNA was extracted and quantified, the IGF2 and MC4R genes were amplified, and the polymerase chain reaction (PCR) produces were digested using the PCR-restriction fragment length polymorphism (PCR-RFLP) technique. Genotyping was performed, and the association between genotypes and growth traits on the birth and weaning weights were evaluated. Results: The IGF2 intron7 g.162G>C variations in Kradon pigs were found in three genotypes: i) GG, ii) GC, and iii) CC. The GG genotype frequency was the highest followed by the GC and CC genotypes. The frequencies of the G and C alleles were 0.703 and 0.297, respectively. The MC4R genotype was found in only one genotype (GG). The IGF2 gene pattern was not associated with birth weight traits, whereas the IGF2 gene pattern was related to the weaning weight trait in Kradon pigs. Pigs with the CC and GC genotypes had higher weaning weights than ones with the GG genotype (p<0.001). Conclusion: Thai native Kradon pigs with the CC and GC genotypes of the IGF2 gene have higher weaning weights than pigs with the GG genotype.
Abstract
Objective: This study was conducted to investigate polymorphisms of the melanocortin-4 receptor (MC4R) and insulin like growth factor 2 (IGF2) genes and to evaluate the growth traits affected by such polymorphisms in Thai native (Kradon) pigs. Methods: Blood samples and productive data from 91 Kradon pigs were collected. DNA was extracted and quantified, the IGF2 and MC4R genes were amplified, and the polymerase chain reaction (PCR) produces were digested using the PCR-restriction fragment length polymorphism (PCR-RFLP) technique. Genotyping was performed, and the association between genotypes and growth traits on the birth and weaning weights were evaluated. Results: The IGF2 intron7 g.162G>C variations in Kradon pigs were found in three genotypes: i) GG, ii) GC, and iii) CC. The GG genotype frequency was the highest followed by the GC and CC genotypes. The frequencies of the G and C alleles were 0.703 and 0.297, respectively. The MC4R genotype was found in only one genotype (GG). The IGF2 gene pattern was not associated with birth weight traits, whereas the IGF2 gene pattern was related to the weaning weight trait in Kradon pigs. Pigs with the CC and GC genotypes had higher weaning weights than ones with the GG genotype (p<0.001). Conclusion: Thai native Kradon pigs with the CC and GC genotypes of the IGF2 gene have higher weaning weights than pigs with the GG genotype.
참고문헌 (33)
- Falvey L, "Research on native pigs in Thailand" (1982), World Animal Review
- Vasupen K, "Nutrition studies in native, Thai Kadon pig", Ph.D, Department of Nutrition, pp.2007
- "Thai native pigs" (2021)[URL]
- Amarger V, "Comparative sequence analysis of the INS-IGF2-H19 gene cluster in pigs", Mamm Genome, 13, pp.388~398 (2002)[DOI]
- O’ Dell SD, "Molecules in focus Insulin-like growth factor II(IGF-II)", Int J Biochem Cell Biol, 30, pp.767~771 (1998)[DOI]
- Kostyunina OV, "The association of IGF2 with productive traits of pigs of large white breed in the aspect of sexual differentiation", Agric Biol, 50, pp.736~745 (2015)[DOI]
- Lamberson WR, "Relationships of serum insulin-like growth factor II concentrations to growth, compositional, and reproductive traits of swine", J Anim Sci, 74, pp.17536~ (1996)[DOI]
- Burgos C, "The effects of two alleles of IGF2 on fat content in pig carcasses and pork", Meat Sci, 90, pp.309~313 (2012)[DOI]
- Thengpimol P, "Association of the Insulin-like Growth Factor II Gene(IGF-II)with growth and body conformation traits in a commercial swine population", Khon Kaen AGR J, 37, pp.319~330 (2009)
- Ampaporn K, "The effects of IGF-II gene on growth trait and carcass quality in Kradon pigs", Khon Kaen AGR J, 46, pp.533~542 (2018)
- Kim KS, "A missense variant of the porcine melanocortin-4 receptor(MC4R)gene is associated with fatness, growth, and feed intake traits", Mamm Genome, 11, pp.131~135 (2000)[DOI]
- Meidtner K, "Association of the melanocortin 4receptor with feed intake and daily gain in F2 Mangalitsa x Pie´train pigs", Anim Genet, 37, pp.245~247 (2006)[DOI]
- Bruun CS, "Evaluation of the porcine melanocortin 4 receptor(MC4R)gene as a positional candidate for a fatness QTL in a cross between Landrace and Hampshire", Anim Genet, 37, pp.359~362 (2006)[DOI]
- Goodwin W, "An introduction to forensic genetics" (2007), John Wiley & Sons Ltd
- Vykoukalová Z, "New SNPs in the IGF2 gene and association between this gene and backfat thickness and lean meat content in Large White pigs", J Anim Breed Genet, 123, pp.204~207 (2006)[DOI]
- Dvorakova V, "Effect of missense mutation Asp298Asn in MC4R on growth and fatness traits in commercial pig crosses in the Czech Republic", Czech J Anim Sci, 56, pp.176~180 (2011)[DOI]
- Nei M, "Estimation of average heterozygosity and genetic distance from a small number of individuals", Genetics, 89, pp.583~590 (1978)[DOI]
- Botstein D, "Construction of genetic linkage map in man using restriction fragment length polymorphisms", Am J Hum Genet, 32, pp.314~331 (1980)
- Klomtong P, "MC1R, KIT, IGF2, and NR6A1 as markers for genetic differentiation in Thai native, wild boars, and Duroc and Chinese Meishan pigs", Genet Mol Res, 14, pp.12723~12732 (2015)[DOI]
- Oczkowicz M, "Effect of IGF2 of intron3-g. 3072G>A on intramuscular fat(IMF)content in pigs raise in Poland", Livest Sci, 149, pp.301~304 (2012)[DOI]
- Clark DL, "Increased prenatal IGF2 expression due to the porcine IGF2 intron3-G3072A mutation may be responsible for increased muscle mass", J Anim Sci, 93, pp.2546~2558 (2015)[DOI]
- Aslan O, "Variation in the IGF2gene promoter region is associated with intramuscular fat content in porcine skeletal muscle", Mol Biol Rep, 39, pp.4101~4110 (2012)[DOI]
- Zhao H, "Liver expression of IGF2 and related proteins in ZBED6 gene-edited pig by RNA-seq", Animals, 10, pp.2184~ (2020)[DOI]
- Younis S, "The ZBED6–IGF2 axis has a major effect on growth of skeletal muscle and internal organs in placental mammals", Proc Natl Acad Sci, 115, pp.e2048~ (2018)[DOI]
- Criado-Mesas L, "Analysis of porcine IGF2 gene expression in adipose tissue and its effect on fatty acid composition", PLoS ONE, 14, pp.e0220708~ (2019)[DOI]
- Braunschweig MH, "Biallelic transcription of the porcine IGF2R gene", Gene, 500, pp.181~185 (2012)[DOI]
- Xiang G, "Editing porcine IGF2 regulatory element improved meat production in Chinese Bama pigs", Cell Mol Life Sci, 75, pp.4619~4628 (2018)[DOI]
- Ruan GR, "Genetic variation at RYR1, IGF2, FUT1, MUC13, and KPL2 mutations affecting production traits in Chinese commercial pig breeds", Czech J Anim Sci, 58, pp.65~70 (2013)
- Chaweewan K, "Association of the melanocortin-4 receptor(MC4R)with economic traits in pigs", Khon Kaen AGR J, 40(Suppl 2), pp.343~350 (2012)
- Fan B, "Detailed characterization of the porcine MC4R gene in relation to fatness and growth", Anim Genet, 40, pp.401~409 (2009)[DOI]
- Szyndler-Nędza M, "Effect of mutation in MC4R gene on carcass quality in Pulawska pig included in conservation breeding programme", Anim Sci Pap Rep, 28, pp.37~45 (2010)
- Piórkowska K, "Association of the melanocortin-4 receptor(MC4R)with feed intake, growth, fatness and carcass composition in pigs raised in Poland", Meat Sci, 85, pp.297~301 (2010)[DOI]
- Van den MK, "The Asp298Asn missense mutation in the porcine melanocortin-4 receptor(MC4R)gene can be used to affect growth and carcass traits without an effect on meat quality", Animal, 1, pp.1089~1098 (2007)[DOI]