Identification of Single Nucleotide Polymorphisms (SNPs) of the Bovine Growth Hormone (bGH) Gene Associated with Growth and Carcass Traits in Hanwoo
1
조회
0
다운로드
2
인용
0
참고문헌
키워드
초록
The purpose of this study was to find any association of the bovine growth hormone (bGH) gene with growth and carcass quality traits in Korean native cattle, Hanwoo. Genomic DNA was extracted from 21 Hanwoo individuals, and the 47 to 2,528 bp region of the bGH 2,856 bp (GenBank accession number M57764) including the promoter and the five exons was sequenced. A total of ten bGH SNPs were confirmed, including four (253 C>T, 303 C>T, 502 C>T, and 559 G>A) in the promoter, one (679 C>T) in exon 1, one (1,692 T>C) in intron 3, and four (2141 C>G, 2258 C>T, 2277 C>T, and 2291 A>C) in exon 5. The ten bGH SNPs were genotyped for a sample of 242 Hanwoo steers and association tests were performed to find any significant SNP that was correlated with growth and carcass quality. Of the SNPs, the 303 C>T SNP in the promoter region was significantly associated with 6-month-old weight, the 559 G>A SNP with longissimus dorsi muscle area, the 2141 C>G SNP in exon 5 with daily weight gain, and the 2258 C>T SNP with daily weight gain and carcass weight (p<0.05). The significant SNPs need to be verified in other Hanwoo populations before considering implementation of marker-assisted selection for genetic improvement of growth and carcass quality in Hanwoo.
Abstract
The purpose of this study was to find any association of the bovine growth hormone (bGH) gene with growth and carcass quality traits in Korean native cattle, Hanwoo. Genomic DNA was extracted from 21 Hanwoo individuals, and the 47 to 2,528 bp region of the bGH 2,856 bp (GenBank accession number M57764) including the promoter and the five exons was sequenced. A total of ten bGH SNPs were confirmed, including four (253 C>T, 303 C>T, 502 C>T, and 559 G>A) in the promoter, one (679 C>T) in exon 1, one (1,692 T>C) in intron 3, and four (2141 C>G, 2258 C>T, 2277 C>T, and 2291 A>C) in exon 5. The ten bGH SNPs were genotyped for a sample of 242 Hanwoo steers and association tests were performed to find any significant SNP that was correlated with growth and carcass quality. Of the SNPs, the 303 C>T SNP in the promoter region was significantly associated with 6-month-old weight, the 559 G>A SNP with longissimus dorsi muscle area, the 2141 C>G SNP in exon 5 with daily weight gain, and the 2258 C>T SNP with daily weight gain and carcass weight (p<0.05). The significant SNPs need to be verified in other Hanwoo populations before considering implementation of marker-assisted selection for genetic improvement of growth and carcass quality in Hanwoo.
참고문헌 (29)
- Astrid Ardiyanti, "Effects of Growth Hormone Gene Polymorphism on Lipogenic Gene Expression Levels in Diaphragm Tissues of Japanese Black Heifers", ASIAN-AUSTRALASIAN JOURNAL OF ANIMAL SCIENCES, 25(8), pp.1055~1062 (2012), 아세아·태평양축산학회[DOI]
- Chikuni, K., "Genetic variants of the growth hormone gene in Japanese cattle", Anim. Sci. Technol. (Jpn.), 65, pp.340~346 (1994)
- Choi, Y. J., "Analysis of restriction fragment length polymorphism in the bovine growth hormone gene related to growth performance and carcass quality of Korean native cattle", Meat. Sci, 45, pp.405~410 (1997)
- Eppard, P. J., "Comparison of the galactopoietic response to pituitary-derived and recombinant-derived variants of bovine growth hormone", J. Endocrinol, 132, pp.47~56 (1992)
- Ferraz, A. L., "Identification and characterization of polymorphisms within the 5' flanking region, first exon and part of first intron of bovine GH gene", J. Anim. Breed. Genet, 123, pp.208~212 (2006)
- Ge, W., "Association of single nucleotide polymorphisms in the growth hormone and growth hormone receptor genes with blood serum insulin-like growth factor I concentration and growth traits in Angus cattle", J. Anim. Sci, 81, pp.641~648 (2003)
- Gordon, D. F., "Nucleotide sequence of the bovine growth hormone chromosomal gene", Mol. Cell. Endocrinol, 33, pp.81~95 (1983)
- Hecht, C., "Variants within the 5′-flanking region and intron I of the bovine growth hormone gene", Anim. Genet, 27, pp.329~332 (1996)
- Hediger, R., "Assignment of the growth hormone gene locus to 19q26-qter in cattle and to 11q25-qter in sheep by in situ hybridization", Genomics, 8, pp.171~174 (1990)
- Herrington, J., "The role of STAT proteins in growth hormone signaling", Oncogene, 19, pp.2585~2597 (2000)
- Ho, K. K., "Aging and growth hormone", Horm. Res, 40, pp.80~86 (1993)
- Kim, N. K., "A previously unreported DraI polymorphism within the regulatory region of the bovine growth hormone gene and its association with growth traits in Korean Hanwoo cattle", Anim. Genet, 35, pp.152~154 (2004)
- Lee, B. K., "Association of somatotropin (BST) gene polymorphism at the 5th exon with selection for milk yield in Holstein cows", Domest. Anim. Endocrinol, 13, pp.373~381 (1996)
- Lincoln, D. T., "Evidence of a direct role for growth hormone (GH) in mammary gland proliferation and lactation", Anat. Histol. Embryol, 24, pp.107~115 (1995)
- Lucy, M. C., "Variants of somatotropin in cattle: gene frequencies in major dairy breeds and associated milk production", Domest. Anim. Endocrinol, 10, pp.325~333 (1993)
- Miller, W. L., "Molecular cloning of DNA complementary to bovine growth hormone mRNA", J. Biol. Chem, 255, pp.7521~7524 (1980)
- Roth, P., "Transcription factor PEA3 participates in the induction of urokinase plasminogen activator transcription in murine kerainocytes stimulated with epidermal growth factor or phorbolester", Nucl. Acid. Res, 18, pp.5009~5017 (1990)
- Sambrook, J., "Molecular cloning: A laboratory manual. Vol. 1 3rd ed", pp.4~12 (2001), Cold Spring Harbor
- Santome, J. A., "The amino acid sequence of bovine growth hormone", FEBS Lett, 16, pp.198~200 (1971)
- Schlee, P., "Growth hormone and insulin-like growth factor I concentrations in bulls of various growth hormone genotypes", Theor. Appl. Genet, 88, pp.497~500 (1994)
- Sumantran, V. N., "Growth hormone induces c-fos and c-jun expression in cells with varying requirements for differentiation", Endocrinology, 130, pp.2016~2024 (1992)
- Theill, L. E., "Transcriptional control of GH expression and anterior pituitary development", Endocrine Rev, 14, pp.670~689 (1993)
- Vaccarelli, G., "Genomic organization and recombinational unit duplication-driven evolution of ovine and bovine T cell receptor gamma loci", BMC Genomic, 9, pp.81~ (2008)
- Wallis, M., "The primary structure of bovine growth hormone", FEBS Lett, 35, pp.11~14 (1973)
- Woychik, R. P., "Cloning and nucleotide sequencing of the bovine growth hormone gene", Nucl. Acid. Res, 10, pp.7197~7210 (1982)
- Yao, J., "Sequence variations in the bovine growth hormone gene characterized by single-strand conformation polymorphism(SSCP)analysis and their association with milk production traits in Holsteins", Genetics, 144, pp.1809~1816 (1996)
- 윤두학, "소 성장호르몬 유전자의 Exon 5번에서의 새로운 다형성 연구", 한국동물자원과학회지, 45(1), pp.13~22 (2003), 한국동물자원과학회
- Zhang, H. M., "Nucleotide sequence determination of a bovine somatotropin allele", Anim. Genet, 23, pp.578~ (1992)
- Zhang, H. M., "Bovine growth hormone gene frequencies in samples of U.S. AI bulls (Abstr.)", J. Anim. Sci, 71, pp.93~ (1993)