제주재래흑돼지와 Landrace의 F2 집단에서 ADCYP1R1, FABP3, MC4R, MYL2 유전자형이 성장형질에 미치는 효과
Effects of ADCYP1R1, FABP3, FABP4, MC4R, MYL2 Genotypes on Growth Traits in F2 Population Between Landrace and Jeju Native Black Pig
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초록
Genetic polymorphisms was investigated at five single nucleotide polymorphisms(SNP) sites in four porcine genes(ADCYAP1R1, FABP3, MC4R, and MYL2) and analyzed their statistical association with growth traits in F2 reciprocal-crossbred population between Landrace and Jeju native black pig(JNP). All populations, JNP, Landrace and their F2 were polymorphic for all five SNP loci tested, however, the homozygote T/T of FABP3 g.-158T>C and the homozygote G/G of ADCYAP1R1 intron 2 337A>G were not found in JNP and Landrace, respectively. The genotypes of ADCYAP1R1 were significantly associated with body weights(BW) at 3 weeks and at 20 weeks(P<0.05), respectivley, those of FABP3 g.-135delT were associated with late average daily gain(LADG) (P<0.01), and those of FABP3 g.-158T>G were associated with body weights during late growth period such as, BW20 and LADG(P<0.01). Those of MC4R were also significantly associated with BW10 suggesting by the difference of early average daily gain(EADG) (P<0.05), and with LADG(P<0.01). The body length of F2 animals was affected by the genotypes of ADCYAP1R1, MC4R, and MYL2(P<0.05), respectively. Among these, MC4R A/A homozygotes showed over 3 cm longer in body length than those of other genotypes. As the useful basic information, these results suggested that SNP markers showing statistical association with growth traits and the results help to select the sires of JNP for improving the productivity in JNP-related crossbreeding system in pig industry and also to construct the molecular breeding system for breed improvement of JNP itself.
Abstract
Genetic polymorphisms was investigated at five single nucleotide polymorphisms(SNP) sites in four porcine genes(ADCYAP1R1, FABP3, MC4R, and MYL2) and analyzed their statistical association with growth traits in F2 reciprocal-crossbred population between Landrace and Jeju native black pig(JNP). All populations, JNP, Landrace and their F2 were polymorphic for all five SNP loci tested, however, the homozygote T/T of FABP3 g.-158T>C and the homozygote G/G of ADCYAP1R1 intron 2 337A>G were not found in JNP and Landrace, respectively. The genotypes of ADCYAP1R1 were significantly associated with body weights(BW) at 3 weeks and at 20 weeks(P<0.05), respectivley, those of FABP3 g.-135delT were associated with late average daily gain(LADG) (P<0.01), and those of FABP3 g.-158T>G were associated with body weights during late growth period such as, BW20 and LADG(P<0.01). Those of MC4R were also significantly associated with BW10 suggesting by the difference of early average daily gain(EADG) (P<0.05), and with LADG(P<0.01). The body length of F2 animals was affected by the genotypes of ADCYAP1R1, MC4R, and MYL2(P<0.05), respectively. Among these, MC4R A/A homozygotes showed over 3 cm longer in body length than those of other genotypes. As the useful basic information, these results suggested that SNP markers showing statistical association with growth traits and the results help to select the sires of JNP for improving the productivity in JNP-related crossbreeding system in pig industry and also to construct the molecular breeding system for breed improvement of JNP itself.
참고문헌 (39)
- Bruun, C. S., "Evaluation of the porcine melanocortin 4 receptor(MC4R) gene as a positional candidate for a fatness QTL in a cross between Landrace and Hampshire", Anim. Genet, 37, pp.359~362 (2006)
- Birren, B., "Genome analysis: A laboratory manual. Vol. 1 Analyzing DNA", pp.6~16 (1997), Cold Spring Harbor Laboratory Press, New York, U.S.A.,
- Choi, Y. S., "Comparison of carcass and meat quality characteristics between Korean native black pigs and commercial crossbred pigs", Korean J. Food Sci. Anim. Resour, 25, pp.322~327 (2005)
- Davoli, R., "Identification of SNPs, mapping and analysis of allele frequencies in two candidate genes for meat production traits: the porcine myosin heavy chain 2B(MYH4) and the skeletal muscle myosin regulatory light chain 2(HUMMLC2B)", Anim. Genet, 34, pp.221~225 (2003)
- Gerbens, F., "Effect of genetic variants of the heart fatty acid-binding protein gene on intramuscular fat and performance traits in pigs", J. Anim. Sci, 77, pp.846~852 (1999)
- Gerbens, F., "Associations of heart and adipocyte fatty acid-binding protein gene expression with intramuscular fat content in pigs", J. Anim. Sci, 79, pp.347~354 (2001)
- Eung Woo Park, "A Large-Insert Porcine Library with Sevenfold Genome Coverage: a Tool for Positional Cloning of Candidate Genes for Major Quantitative Traits", Molecules and Cells, 16(1), pp.113~116 (2003), 한국분자세포생물학회
- Jokubka, R., "Association of a melanocortin 4 receptor(MC4R) polymorphism with performance traits in Lithuanian White pigs", J. Anim. Breed. Genet, 123, pp.17~22 (2006)
- Kim, N. E., "Discovery of single nucleotide polymorphisms in SLA-1 cDNA from Korean native pigs and phylogenetic analysis", Korean J. Genetics, 28, pp.353~360 (2006)
- Kim, K. I., "Phylogenetic relationships of Asian and European pig breeds determined by mitochondrial DNA D-loop sequence polymorphism", Anim. Genet, 33, pp.19~25 (2002)
- Kim, K. S., "A missense variant of the porcine melanocortin-4 receptor(MC4R) gene is associated with fatness, growth, and feed intake traits", Mamm. Genome, 11, pp.131~135 (2000)
- Kim, K. S., "Assessment of genetic diversity of Korean native pig(Sus scrofa) using AFLP markers", Genes Genet. Syst, 77, pp.361~368 (2002)
- Kim, T. H., "Genetic structure of pig breeds from Korea and China using microsatellite loci analysis", J. Anim. Sci, 83, pp.2255~2263 (2005)
- Kollers, S., "Single nucleotide polymorphism identification, linkage and radiation hybrid mapping of the porcine pituitary adenylate cyclase-activating polypeptide type I receptor gene to chromosome 18", J. Anim. Breed. Genet, 123, pp.414~418 (2006)
- Kwon, O. S., "Korean native black pigs. Standard guideline for farmer" (2001), Rural Development Administration
- Lee, Y. J., "Sequence- based characterization of the eight SLA loci in Korean native pigs", Int. J. Immunogenet, 35, pp.333~334 (2008)
- Meidtner, K., "Association of the melanocortin 4 receptor with feed intake and daily gain in F2 Mangalitsa × Pietrain pigs", Anim. Genet, 37, pp.245~247 (2006)
- Park, H. B., "Melanocortin-4 receptor (MC4R) genotypes have no major effect on fatness in a Large White x Wild Boar intercross", Anim. Genet, 33, pp.155~157 (2002)
- STAT software for PC, "SAS", pp.1999, SAS
- Woo, C. J., "Allelic variation and phylogenetic analysis of SLA-3 between Korean native pigs and other pig breeds", Korean J. Genetics, 28, pp.305~310 (2006)
- Woo. C. J, "Molecular cloning and analysis of allelic variation of the SLA-2 gene in Korean native pigs", Korean J. Genetics, 27, pp.337~343 (2005)
- Xu, D. Q., "Identification of polymorphisms and association analysis with meat quality traits in the porcine KIAA1717 and HUMMLC2B genes", Livest. Sci, 106, pp.96~101 (2007)
- 재래돼지 표현형적 특성 및 핵집단 조성연구. 축산시험연구보고서. 제2권, "권오섭", 878~ (1998)
- 조병욱, "돼지 melanocortin-4 receptor (MC4R) 유전자의 경제형질과의 연관성에 관한 연구", 생명과학회지, 15(6), pp.968~971 (2005), 한국생명과학회
- 김명직, "Microsatellite Marker를 이용한 한국재래돼지 집단의 품종특성 및 원산지 추적을 위한 개체식별체계 설정", 한국축산식품학회지, 27(2), pp.150~156 (2007), 한국축산식품학회
- 김재환, "돼지 FABP3 promoter 부위 내 신규 돌연변이 탐색과 근내지방도와의 연관성 분석", 한국동물자원과학회지, 47(1), pp.1~10 (2005), 한국동물자원과학회
- 김태헌, "재래돼지 Melanocortin Receptor 1(MCIR)의 변이에 관한 연구", 한국동물자원과학회지, 43, pp.293~302 (2001)
- 서보영, "대한민국내 주요 돼지 품종의 순종 식별을 위한 품종특이 DNA marker의 활용", 한국동물자원과학회지, 46(5), pp.735~742 (2004), 한국동물자원과학회
- 이성수, "재래 흑돼지와 중국 재래돈간의 Melanocortin Receptor 1(MC1R) 유전자의 유전자형 분석", 한국동물자원과학회지, 43, pp.1~8 (2001)
- 정의룡, "AFLP Marker 를 이용한 한국 재래돼지의 유전적 다양성 및 품종식별", 한국동물자원과학회지, 43, pp.777~788 (2001)
- 조규호, "듀록 품종의 Melanocortin-4 Receptor(MC4R) 유전자와 성장형질과의 연관성 분석", 한국동물자원과학회지, 49(4), pp.437~442 (2007), 한국동물자원과학회
- 조수현, "재래돼지의 출하체중별 도체수율 및 육질특성", 한국동물자원과학회지, 49(4), pp.523~530 (2007), 한국동물자원과학회
- 조용민, "개체별 성장곡선 모수를 이용한 재래돼지와 랜드레이스종의 성장 특성에 관한 연구", 한국동물자원과학회지, 43, pp.817~822 (2001)
- 조인철, "랜드레이스, 대요크셔, 듀록 및 제주 흑돈의 Melanocortin 1Receptor(MC1R) 유전자의 유전자형 분석", 한국동물자원과학회지, 44(2), pp.207~212 (2002), 한국동물자원과학회
- 조인철, "돼지 Melanocortin Receptor 1(MC1R) 대립유전자 3의 신규 유전변이 탐색", 한국동물자원과학회지, 46(1), pp.1~6 (2004), 한국동물자원과학회
- 진상근, "랜드레이스와 재래돼지육의 이화학적 특성", 한국축산식품학회지, 21, pp.142~148 (2001)
- 한상현, "제주재래돼지 집단에서 집단특이적 mtDNA haplotype과 유전적 다양성", 한국동물자원과학회지, 46(6), pp.917~924 (2004), 한국동물자원과학회
- 한상현, "돼지 MC4R 유전자 892G>A 다형성이 비육돈의 도체형질에 미치는 영향", 한국동물자원과학회지, 49(5), pp.569~576 (2007), 한국동물자원과학회
- 한상현, "비육돈의 도체형질과 MYL2, ADCYAP1R1 유전자 다형성의 상관관계", 한국동물자원과학회지, 50(3), pp.301~308 (2008), 한국동물자원과학회