Assessment of lactic acid bacteria isolated from the chicken digestive tract for potential use as poultry probiotics
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Objective: The use of probiotics as an alternative to antibiotics in animal feed has received considerable attention in recent decades. Lactic acid bacteria (LAB) have remarkable functional properties promoting host health and are major microorganisms for probiotic purposes. The aim of this study was to characterize LAB strains of the chicken digestive tract and to determine their functional properties for further use as potential probiotics in poultry. Methods: A total of 2,000 colonies were isolated from the ileum and cecal contents of the chickens based on their phenotypic profiles and followed by a preliminary detection for acid and bile tolerance. The selected 200 LAB isolates with exhibited well-tolerance in acid and bile conditions were then identified by sequencing the 16S rDNA gene, followed by acid and bile tolerance, antimicrobial activity, adhesion to epithelial cells and additional characteristics on the removal of cholesterol. Then, the two probiotic strains (L. ingluviei and L. salivarious) which showed the greatest advantage in vitro testing were selected to assess their efficacy in broiler chickens. Results: It was found that 200 LAB isolates that complied with all measurement criteria belonged to five strains, including L. acidophilus (63 colonies), L. ingluviei (2 colonies), L. reuteri (58 colonies), L. salivarius (72 colonies), and L. saerimneri (5 colonies). We found that the L. ingluviei and L. salivarius can increase the population of LAB and Bifidobacterium spp. while reducing Enterobacteria spp. and Escherichia coli in the cecal content of chickens. Additionally, increased concentrations of valeric acid and short chain fatty acids were also observed. Conclusion: This study indicates that all five Lactobacillus strains isolated from gut contents of chickens are safe and possess probiotic properties, especially L. ingluviei and L. salivarius. Future studies should evaluate the potential for growth improvement in broilers.
Abstract
Objective: The use of probiotics as an alternative to antibiotics in animal feed has received considerable attention in recent decades. Lactic acid bacteria (LAB) have remarkable functional properties promoting host health and are major microorganisms for probiotic purposes. The aim of this study was to characterize LAB strains of the chicken digestive tract and to determine their functional properties for further use as potential probiotics in poultry. Methods: A total of 2,000 colonies were isolated from the ileum and cecal contents of the chickens based on their phenotypic profiles and followed by a preliminary detection for acid and bile tolerance. The selected 200 LAB isolates with exhibited well-tolerance in acid and bile conditions were then identified by sequencing the 16S rDNA gene, followed by acid and bile tolerance, antimicrobial activity, adhesion to epithelial cells and additional characteristics on the removal of cholesterol. Then, the two probiotic strains (L. ingluviei and L. salivarious) which showed the greatest advantage in vitro testing were selected to assess their efficacy in broiler chickens. Results: It was found that 200 LAB isolates that complied with all measurement criteria belonged to five strains, including L. acidophilus (63 colonies), L. ingluviei (2 colonies), L. reuteri (58 colonies), L. salivarius (72 colonies), and L. saerimneri (5 colonies). We found that the L. ingluviei and L. salivarius can increase the population of LAB and Bifidobacterium spp. while reducing Enterobacteria spp. and Escherichia coli in the cecal content of chickens. Additionally, increased concentrations of valeric acid and short chain fatty acids were also observed. Conclusion: This study indicates that all five Lactobacillus strains isolated from gut contents of chickens are safe and possess probiotic properties, especially L. ingluviei and L. salivarius. Future studies should evaluate the potential for growth improvement in broilers.
참고문헌 (44)
- Mottet A, "Global poultry production : current state and future outlook and challenges", World Poult Sci J, 73, pp.245~256 (2017)[DOI]
- Aruwa CE, "Poultry gut health – microbiome functions, environmental impacts, microbiome engineering and advancements in characterization technologies", J Anim Sci Biotechnol, 12, pp.119~ (2021)[DOI]
- Ahmad R, "Influence of heat stress on poultry growth performance, intestinal inflammation, and immune function and potential mitigation by probiotics", Animals, 12, pp.2297~ (2022)[DOI]
- Clavijo V, "The gastrointestinal microbiome and its association with the control of pathogens in broiler chicken production : a review", Poult Sci, 97, pp.1006~1021 (2018)[DOI]
- Hill C, "Expert consensus document : the international scientific association for probiotics and prebiotics consensus statement on the scope and appropriate use of the term probiotic", Nat Rev Gastroenterol Hepatol, 11, pp.506~514 (2014)[DOI]
- Ashaolu TJ, "Immune boosting functional foods and their mechanisms : a critical evaluation of probiotics and prebiotics", Biomed Pharmacother, 130, pp.110625~ (2020)[DOI]
- Chen S, "Gut microbiota implications for health and welfare in farm animals : a review", Animals, 12, pp.93~ (2022)[DOI]
- Heravi RM, "Screening of lactobacilli bacteria isolated from gastrointestinal tract of broiler chickens for their use as probiotic", Afr J Microbiol Res, 5, pp.1858~1868 (2011)
- Noohi N, "Screening for probiotic characters in lactobacilli isolated from chickens revealed the intra-species diversity of Lactobacillus brevis", Anim Nutr, 7, pp.119~126 (2021)[DOI]
- Al-Khalaifa H, "Effect of dietary probiotics and prebiotics on the performance of broiler chickens", Poult Sci, 98, pp.4465~4479 (2019)[DOI]
- Kizerwetter-Świda M, "Assessment of potentially probiotic properties of Lactobacillus strains isolated from chickens", Pol J Vet Sci, 19, pp.15~20 (2016)[DOI]
- Ehrmann MA, "Characterization of lactobacilli towards their use as probiotic adjuncts in poultry", J Appl Microbiol, 92, pp.966~975 (2002)[DOI]
- Krysiak K, "Overview of the use of probiotics in poultry production", Animals, 11, pp.1620~ (2021)[DOI]
- Noohi N, "Screening for probiotic characters in lactobacilli isolated from chickens revealed the intra-species diversity of Lactobacillus brevis", Anim Nutr, 7, pp.119~126 (2021)[DOI]
- Walker DK, "Relationships among bile tolerance, bile salt deconjugation, and assimilation of cholesterol by Lactobacillus acidophilus", J Dairy Sci, 76, pp.956~961 (1993)[DOI]
- Nami Y, "Probiotic potential and biotherapeutic effects of newly isolated vaginal Lactobacillus acidophilus 36YL strain on cancer cells", Anaerobe, 28, pp.29~36 (2014)[DOI]
- Pennacchia C, "Potential probiotic Lactobacillus strains from fermented sausages : further investigations on their probiotic properties", Meat Sci, 73, pp.90~101 (2006)[DOI]
- Liong MT, "Acid and bile tolerance and cholesterol removal ability of lactobacilli strains", J Dairy Sci, 88, pp.55~66 (2005)[DOI]
- National Research Council, "Nutrient requirements of poultry" (1994), National Academies Press
- Cobb-Vantress, "Cobb 500 broiler performance and nutrition supplement" (2018), Cobb-Vantress Inc
- Sharmila A, "Quantitative real-time PCR analysis of the caecal bacteria population of broiler chickens fed with corn-soy diet containing 20% of palm kernel meal with or without enzyme supplementation", J Anim Poult Sci, 4, pp.1~9 (2015)
- Mookiah S, "Effects of dietary prebiotics, probiotic and synbiotics on performance, caecal bacterial populations and caecal fermentation concentrations of broiler chickens", J Sci Food Agric, 94, pp.341~348 (2014)[DOI]
- Dowarah R, "Selection and characterization of probiotic lactic acid bacteria and its impact on growth, nutrient digestibility, health and antioxidant status in weaned piglets", PloS one, 13, pp.e0192978~ (2018)[DOI]
- Mountzouris KC, "Evaluation of the efficacy of a probiotic containing Lactobacillus, Bifidobacterium, Enterococcus, and Pediococcus strains in promoting broiler performance and modulating cecal microflora composition and metabolic activities", Poult Sci, 86, pp.309~317 (2007)[DOI]
- Piqué N, "Health benefits of heat-killed(Tyndallized)probiotics : an overview", Int J Mol Sci, 20, pp.2534~ (2019)[DOI]
- Morelli L, "In vitro selection of probiotic lactobacilli : a critical appraisal", Curr Issues Intest Microbiol, 1, pp.59~67 (2000)
- Svihus B, "Function of the digestive system", J Appl Poult Res, 23, pp.306~314 (2014)[DOI]
- Hutari A, "Screening of Lactobacillus strains against Salmonella both isolated from Malaysian free-range chicken intestine for use as probiotic", Sains Malays, 40, pp.1115~1122 (2011)
- Erkkilä S, "Screening of commercial meat starter cultures at low pH and in the presence of bile salts for potential probiotic use", Meat Sci, 55, pp.297~300 (2000)[DOI]
- Pennacchia C, "Selection of Lactobacillus strains from fermented sausages for their potential use as probiotics", Meat Sci, 67, pp.309~317 (2004)[DOI]
- Vieco-Saiz N, "Benefits and inputs from lactic acid bacteria and their bacteriocins as alternatives to antibiotic growth promoters during foodanimal production", Front Microbiol, 10, pp.57~ (2019)[DOI]
- Onwuakor CE, "Effect of varied culture conditions on crude supernatant(bacteriocin)production from four Lactobacillus species isolated from locally fermented maize(ogi)", Am J Microbiol Res, 2, pp.125~130 (2014)[DOI]
- Dec M, "Assessment of antibiotic susceptibility in Lactobacillus isolates from chickens", Gut Pathog, 9, pp.54~ (2017)[DOI]
- Jose NM, "Comparison of microbiological and probiotic characteristics of lactobacilli isolates from dairy food products and animal rumen contents", Microorganisms, 3, pp.198~212 (2015)[DOI]
- Monteagudo-Mera A, "Adhesion mechanisms mediated by probiotics and prebiotics and their potential impact on human health", Appl Microbiol Biotechnol, 103, pp.6463~6472 (2019)[DOI]
- Pringsulaka O, "In vitro screening of lactic acid bacteria for multi-strain probiotics", Livest Sci, 174, pp.66~73 (2015)[DOI]
- Lye HS, "Removal of cholesterol by lactobacilli via incorporation and conversion to coprostanol", J Dairy Sci, 93, pp.1383~1392 (2010)[DOI]
- Angelakis E, "An evaluation of the effects of Lactobacillus ingluviei on body weight, the intestinal microbiome and metabolism in mice", Microb Pathog, 52, pp.61~68 (2012)[DOI]
- Shokryazdan P, "Probiotic potential of lactic acid bacteria isolated from mulberry silage", Life Sci J, 18, pp.35~45 (2021)[DOI]
- Sureshkumar S, "Inclusion of Lactobacillus salivarius strain revealed a positive effect on improving growth performance, fecal microbiota and immunological responses in chicken", Arch Microbiol, 203, pp.847~853 (2021)[DOI]
- Deleu S, "Short chain fatty acids and its producing organisms : An overlooked therapy for IBD?", EBioMedicine, 66, pp.103293~ (2021)[DOI]
- Tsukagoshi M, "Lactobacillus ingluviei C37 from chicken inhibits inflammation in LPSstimulated mouse macrophages", Anim Sci J, 91, pp.e13436~ (2020)[DOI]
- Qing Y, "Gut microbiome, short-chain fatty acids, and mucosa injury in young adults with human immunodeficiency virus infection", Dig Dis Sci, 64, pp.1830~1843 (2019)[DOI]
- Onrust L, "Valeric acid glyceride esters in feed promote broiler performance and reduce the incidence of necrotic enteritis", Poult Sci, 97, pp.2303~2311 (2018)[DOI]
