닭의 맹장에서 분리된 유산균의 생균제적 특성
Characterization of Lactobacilli Isolated from Chicken Ceca as Probiotics
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This experiment was conducted to investigate enzyme activity, antimicrobial activity, and antibiotics susceptibility of Lactobacilli strain(Lactobacillus reuteri BLA5, Lactobacillus crispatus BLA7, Lactobacillus reuteri BLA9, Lactobacillus amylovorus LLA7, Lactobacillus crispatus LLA9, Lactobacillus vaginalis LLA11) isolated from chicken ceca and were selected by organic acid synthesis, acid tolerance, bile salt tolerance. The enzymes activities were different among strains of Lactobacilli. The amylase activity and lipase activity of Lactobacillus were high but cellulase activity and protease activity of that were low. Lactobacillus culture showed high antimicrobial activity against E. coli but low antimicrobial activity against Salmonella. The inhibitory factor of Lactobacilli isolated from chickens’ cecum on E. coli was low pH by organic acid. All of Lactobacillus isolated from chicken’s cecum were susceptible to ampicillin and amoxicillin but weren’t susceptible at the optimum level of feed additive antibiotics(virginiamycin and salinomycin).
Abstract
This experiment was conducted to investigate enzyme activity, antimicrobial activity, and antibiotics susceptibility of Lactobacilli strain(Lactobacillus reuteri BLA5, Lactobacillus crispatus BLA7, Lactobacillus reuteri BLA9, Lactobacillus amylovorus LLA7, Lactobacillus crispatus LLA9, Lactobacillus vaginalis LLA11) isolated from chicken ceca and were selected by organic acid synthesis, acid tolerance, bile salt tolerance. The enzymes activities were different among strains of Lactobacilli. The amylase activity and lipase activity of Lactobacillus were high but cellulase activity and protease activity of that were low. Lactobacillus culture showed high antimicrobial activity against E. coli but low antimicrobial activity against Salmonella. The inhibitory factor of Lactobacilli isolated from chickens’ cecum on E. coli was low pH by organic acid. All of Lactobacillus isolated from chicken’s cecum were susceptible to ampicillin and amoxicillin but weren’t susceptible at the optimum level of feed additive antibiotics(virginiamycin and salinomycin).
참고문헌 (25)
- Afrin, R., "Catalytic activity of Teflon particle-immobilized protease in aqueous solution", J. Molecular Catalysis, 9, pp.259~267 (2000)
- Barrow, P. A., "The attachment of bacteria to the epithelium of the pig and its importance in the microecology of the intestine", J. Appl. Bact, 48, pp.147~ (1980)
- Bauer, A. W., "Antibiotic susceptibility testing by a standardized single disk method", Am. J. Clin. Pathol, 45, pp.493~496 (1966)
- Bongaerts, G., "Effect of antibiotics, prebiotics and Pro- biotics in treatment for hepatic encephalopathy", Med. Hypotheses, 64, pp.64~68 (2005)
- Dunham, H. J., "Lactobacillus reuteri immunomodulation of stressor-associated disease in newly hatched chickens and turkeys", Poult. Sci, 72(Suppl 1), pp.103~ (1993)
- Fuller, R, "Ecological studies on the Lactobacillus flora associated with the crop epithelium of the fowl", J. Appl. Bacteriol, 36, pp.131~139 (1973)
- Fuller, R, "Nature of the determinant responsible for the adhesion of lactobacilli to chicken crop epithelial cells", Fournal of General Microbiology, 87, pp.245~250 (1975)
- Fuller, R, "Probiotics in man and animals", J. Appl. Bacteriol, 66, pp.365~378 (1989)
- Gibson, G. R, "Aspects of in vitro and in vivo research approaches directed toward identifying probiotics and prebiotics for human use", J. Nutr, 130, pp.391~395 (2000)
- Gilliland, S. E, "Beneficial interrelationships between certain micro-organisms and humans: Candidate micro-organisms for use as dietary adjuncts", J. Food Protect, 42, pp.164~ (1979)
- Hernandez, F., "Influence of two plant extracts on broilers performance, digestibility, and organ size", Poult. Sci, 83, pp.169~174 (2004)
- Jennson, B, "The possibility of manipulation of microbial activity in the digestive tract of monogastric animals", Proceedings of the 44th annual meeting for animal production, pp.313~ (1993)
- Jin, L. Z., "Antagonistic effects of intestinal Lactobacillus isolates on pathogens of chicken", Letters in Applied Microbiology, 23, pp.67~71 (1996)
- Klaenhammer, T. R, "Bacteriocins of lactic acid bacteria", Biochimie, 70, pp.337~349 (1988)
- Miller, G. L., "Use of dinitrosalicyclic acid reagent for determination of reducing sugar", Anal. Chem, 31, pp.426~428 (1959)
- Mohan, B., "Effect of probiotic supplementation on growth nitrogen utilization and serum cholesterol in broilers", Br. Poult. Sci, 37, pp.395~401 (1996)
- Morishita, Y., "Influence of dietary lactose on the gut flora of chicks", Br. Poult. Scie, 23, pp.349~359 (1982)
- Nurmi, E., "New aspects of Salmonella infection in broiler production", Nature, 241, pp.210~211 (1973)
- Ransac, S., "Crystalliza subtilis", J. Molecular Biology, 238, pp.857~859 (1994)
- Rubio, L. A., "Lactobacilli counts in crop ileum and caecum of growing broiler chickens fed on practical diets containing whole or dehulled sweet lupin Lupinus angustifolius seed meal", Br. Poult. Sci, 39, pp.354~359 (1998)
- Spencer, R. J., "The effect of Lactobacillus spp. on the attachment of enterotoxigenic Escherichia coli to isolated porcine enterocytes", Fournal of Applied Bacteriology, 77, pp.215~220 (1994)
- Tagg, J. R., "Bacteriocins of Gram-positive bacteria", Bacteriological Reviews, 40, pp.722~756 (1976)
- Watkins, B. A., "In vivo effects of Lactobacillus acidophilus against pathogenic Escherichia coli in gnotobiotic chicks", Poult. Sci, 61, pp.1298~1308 (1982)
- Watkins, B. A., "Effect of oral dosing of Lactobacillus strains on gut colonization and liver biotin in broiler chicks", Poult. Sci, 62, pp.2088~2094 (1983)
- White, F., "Stomach function in relation to a scour syndrome in the piglet", Br. J. Nutr, 23, pp.847~858 (1969)