Characterization and Evaluation of Potential Probiotic Lactobacillus delbrueckii Isolated from Human Feces: Using In vitro Analysis
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The use of probiotic microbes to prevent and treat diseases and promote the health is one of the fundamental areas of research in microbiology. Present study was aimed to investigate the probiotic potential of Lactobacillus strains isolated from feces of the adult humans. Isolated strains were identified and assessed for their resistance to acid and bile, tolerance to gastric fluid and pancreatic fluid, antimicrobial activity, bile salt hydrolase(BSH) activity, antibiotic susceptibility, cholesterol assimilation and surface hydrophobicity as probiotic characteristics. Bile acids and low pH were tolerated by three strains of Lactobacillus delbrueckii. These strains possessed antimicrobial activity against selected harmful microorganisms and were sensitive to penicillin, chloramphenicol, and gentamicin. The strains were proven non-virulent by bile salt hydrolase activity and absence of DNase activity. Moreover, these strains exhibited higher levels of cholesterol assimilation and surface hydrophobicity. Therefore, present research emphasize that these Lactobacillus strains can be promising probiotic candidates for application as functional foods.
Abstract
The use of probiotic microbes to prevent and treat diseases and promote the health is one of the fundamental areas of research in microbiology. Present study was aimed to investigate the probiotic potential of Lactobacillus strains isolated from feces of the adult humans. Isolated strains were identified and assessed for their resistance to acid and bile, tolerance to gastric fluid and pancreatic fluid, antimicrobial activity, bile salt hydrolase(BSH) activity, antibiotic susceptibility, cholesterol assimilation and surface hydrophobicity as probiotic characteristics. Bile acids and low pH were tolerated by three strains of Lactobacillus delbrueckii. These strains possessed antimicrobial activity against selected harmful microorganisms and were sensitive to penicillin, chloramphenicol, and gentamicin. The strains were proven non-virulent by bile salt hydrolase activity and absence of DNase activity. Moreover, these strains exhibited higher levels of cholesterol assimilation and surface hydrophobicity. Therefore, present research emphasize that these Lactobacillus strains can be promising probiotic candidates for application as functional foods.