원심성 운동이 제 2형 당뇨쥐의 인슐린 저항성과 골격근 GLUT-4 발현에 미치는 영향
Effect of eccentric exercise on insulin resistance and skeletal muscle GLUT-4 protein expression of type Ⅱ diabetes rats
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본 연구의 목적은 제 2형 당뇨를 가진 쥐를 대상으로 원심성 운동을 처치함으로써, 원심성 운동이 인슐린 저항성과 soleus와 triceps 골격근 GLUT-4 protein 발현에 미치는 영향을 측정하고 분석하는 것이다. 7주령 된 Wistar & GK Rat 총 25마리를 분양받아 각각 NDM-NEX, NDM-EX, DM-NEX, DM-EX의 네 집단으로 배정하였다. 실험동물을 1주일 동안 실험실 환경에 적응 시킨 후, 12시간동안 공복을 시켜 -16degree에서 16m/min으로 1시간 동안 운동처치를 시켰다. 운동직후, 30분, 60분, 90분, 120분의 OGTT 검사를 통하여 인슐린 저항성을 측정하였다. OGTT 중 복용시킨 glucose가 GLUT-4 expression level에 영향을 미칠 것을 감안하여, 1주일간의 wash-out period를 가진 후 다시 운동처치를 실시하였다. 본 연구 결과, 원심성 운동 직후 혈당의 변화에서는 DM군과 NDM군 모두 운동 후 운동전에 비해 높은 혈당을 나타내었다. 또한 인슐린 저항성에서는 NDM-EX군이 NDM-NEX군에 비해 120분 동안 계속적으로 높은 인슐린 저항성을 나타낸 반면, DM-EX군은 DM-NEX군에 비해 30분까지만 높은 인슐린 저항성을 나타내다가 이후에는 낮은 인슐린 저항성을 나타내었다. 한편 당뇨여부, 원심성 운동여부, 그리고 당뇨여부와 원심성 운동여부를 동시에 고려하더라도 soleus와 triceps의 GLUT-4 protein expression 수준에는 차이가 나타나지 않았다. 결론적으로, 이 연구에서 나타난 결과는 원심성 운동으로 인해 나타난 인슐린 저항성은 DM group과 NDM group간에 다른 양상을 나타내었다.
Abstract
The purpose of this study was to investigate the insulin resistance and GLUT-4 protein expression in soleus and triceps according to downhill treadmill exercise in rats. 7-week-old, Wistar & GK rats were randomly divided into Non type 2 diabetes․Non exercise(NDM-NEX), Non type 2 diabetes․Exercise (NDM-EX), type 2 Diabetes․Non exercise(DM-NEX), type 2 Diabetes․Exercise(DM-EX). Rats in NDM-EX and DM-EX group were forced to run on he treadmill at the -16 degree and speed of 16m/min for 1 hour. All group rats were incubated with 2ml/kg glucose 20% solution after exercise and blood samples were taken from the tail at 0(before injection), 30, 60, 90 and 120 min after the oral glucose load for blood glucose. After 1 week wash-out period, rats in NDM-EX and DM-EX group were forced to run on he treadmill at the -16 degree and speed of 16m/min for 1 hour. After eccentric exercise in blood glucose, DM and NDM group showed high that compared before exercise to after exercise. In addition, compared with NDM-NEX group, NDM-EX group showed high insulin resistance during 120 min in insulin. On the other hand compared with DM-NEX group, DM-EX group showed high insulin resistance during 30 min and low insulin resistance after 30min in insulin resistance. In conclusion, the new finding of this study was that insulin resistance by down-hill treadmill exercise significantly different between DM group and NDM group.
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