지구성 운동강도 차이가 streptozotocin 유발 당뇨쥐의 인슐린 및 골격근 제4형 당수송체(GLUT-4) 발현에 미치는 영향
The effect of exercise intensity on blood insulin and GLUT-4 protein expression of skeletal muscle in STZ-diabetic rats
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지구성 운동강도 차이가 streptozotocin 유발 당뇨쥐의 인슐린 및 골격근 제4형 당수송체(GLUT-4) 발현에 미치는 영향.운동과학, 제13권 2호, 211-222, 2004. 본 연구는 지구성 운동부하 강도가 혈당, 인슐린 농도, 그리고 골격근 가자미근의 제4형 당수송체(GLUT-4) 발현에 미치는 영향을 규명하기 위하여 Sprague-Dawley계 수컷 흰쥐를 무작위 표본추출에 의하여 정상군과 당뇨유발군, 당뇨유발 저강도운동군, 그리고 당뇨유발 고강도운동군으로 분류하였다. 저강도운동은 트레드밀 경사도 0 %에서 10 m/min 속도로 실시하였으며, 고강도운동은 트레드밀 경사도 0%에서 24 m/min 속도로 실시하였다. 운동은 주당 5회, 운동시간은 하루 일회씩 30분씩 4주 동안 실시하였다. 체중은 집단(p<.001), 운동기간(p<.001), 그리고 집단 및 운동기간(p<.001)의 상호작용에서 유의한 차이가 나타났다. 정상군에서 지속적으로 유의하게 증가하였으며, 운동 3주와 4주에서는 정상군과 당뇨유발군, 당뇨유발 저강도, 고강도운동군 사이에 유의한 차이가 나타났다. 혈당은 집단(p<.001), 운동기간(p<.001), 그리고 집단 및 운동기간(p<.05)의 상호작용에서 유의한 차이가 나타났다. 모든 당뇨유발군이 정상군보다 유의하게 높게 유지 되었으며, 운동 3주 4주에는 당뇨유발 저강도운동군이 당뇨군, 당뇨유발 고강도운동군보다 유의하게 낮게 나타났다. 인슐린 농도는 집단(p<.001) 간에 유의한 차이가 나타났으며, 정상군과 당뇨유발 저강도운동군은 당뇨유발 고강도운동군보다 당뇨유발 고강도운동군은 당뇨유발군보다 유의하게 높게 나타났다. GLUT-4 단백질 발현은 정상군에 비해서 당뇨유발군에서 감소한 것으로 나타났으나, 당뇨유발 고강도운동군에서 다소 증가하였으며, 당뇨유발 저강도운동군에서 정상수준으로 증가한 것으로 나타났다. 이상의 결과로 보아 트레드밀 지구성 운동은 고강도운동보다 저강도운동이 당뇨유발에 의해 악화된 혈당과 인슐린 농도에 긍정적인 작용을 하며, 이러한 기전의 하나로 운동이 당뇨유발에 의해 감소된 골격근 당수송체의 양을 증가시키는 것으로 생각된다.
Abstract
The effect of exercise intensity on blood insulin and GLUT-4 protein expression of skeletal muscle in STZ-diabetic rats. Exercise Science, 13(2): 211-222, 2004. The pupose of this study was to investigate the effect of exercise intensity on blood insulin and glucose transporter 4 (GLUT-4) protein expression of skeletal muscle in STZ-diabetic rats. Male Sprague-Dawley rats were randomly divided into normal, diabetes(DM), diabetic low-intensity exercise(DLE), and diabetic high-intensity exercise(DHE) groups. Animals of the exercise groups were put on running for 30 min once a day, 5days per week for 4 weeks. The regimen of low-intensity exercise consisted with running at speed of 10 m/min, high- intensity exercise was 24 m/min, on 0% grade. In the present results, weight gain of the rats in DM, DLE, and DHE groups was significantly lowered compared to normal group, and decreased by exercise. Plasma glucose concentration in DM, DLE, and DHE groups was significantly higher than that of normal group, the concentration of DLE group was significantly lower than that of DM, DHE group in 3 and 4 weeks. Plasma insulin concentration in normal, DLE groups was significantly highered in compared to DM, DHE groups, and the conce- ntration of DHE group was significantly higher than that of DM group. GLUT-4 protein level of soleus in DM group was remarkably decreased compared to normal group, but the level was recovered to the level of normal group by 4 week low-intensity exercise. In conclusion, the insulin resistance induced by STZ administration was partially improve by 4 week low-intensity endurance exercise.
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