본문 바로가기
KERD
논문KCI 등재

8주간의 저항성운동 및 열량 감소식이가 비만 중년 흰쥐의 Traf2- NFkB-mTOR 및 SIRT1-FoxO1 신호인자의 발현에 미치는 영향

Effects of 8 Weeks Calorie Reduction and Resistance Exercise on Traf2-NFkB-mTOR and SIRT1-FoxO1 Signal Expression of Cardiac Muscle in High-fat Induced Obese Middle-Aged Rats

1

조회

0

다운로드

0

인용

0

참고문헌

키워드

초록

PURPOSE: The purpose of this study was to investigate the effect of 8-week calorie reduction and resistance exercise on the expression of Traf2-NFkB-mTOR and SIRT1-FoxO1 signal of cardiac muscle in obese middle aged rats. METHODS: 50 weeks old male Wistar rats were induced to obese by high fat diet treatment for 6 weeks after 1 week of environmental adaptation and then were randomly assigned to 4 groups (control group, CON; diet control with calorie reduction group, DC; resistance exercise group, RE; diet control and resistance exercise group, DRE). After that ladder climbing exercise or calorie reduction with general formula were treated for 8 weeks. RESULTS: Body weight and abdominal fat were significantly (p<.05) lower in the two groups as DC and DRE than the other groups as CON and RE. There was no significant difference in the weight of heart and skeletal muscle. Eight weeks of exercise or caloric reduction diet significantly (p<.05) reduced gene expression and activity of Traf2-NFkB-mTOR, which is a hypertrophic factor of cardiac muscle in obese middle-aged rats, and that autophagy factors of SIRT1-FoxO1 gene expression and activity were significantly (p<.05) increased. However, there was no synergistic effect with the combined treatment of the two stimuli. CONCLUSIONS: These results suggest that caloric reduction with weight loss or resistance training may be effective in preventing cardiac hypertrophy and heart failure in middle-aged obese rats.

Abstract

PURPOSE: The purpose of this study was to investigate the effect of 8-week calorie reduction and resistance exercise on the expression of Traf2-NFkB-mTOR and SIRT1-FoxO1 signal of cardiac muscle in obese middle aged rats. METHODS: 50 weeks old male Wistar rats were induced to obese by high fat diet treatment for 6 weeks after 1 week of environmental adaptation and then were randomly assigned to 4 groups (control group, CON; diet control with calorie reduction group, DC; resistance exercise group, RE; diet control and resistance exercise group, DRE). After that ladder climbing exercise or calorie reduction with general formula were treated for 8 weeks. RESULTS: Body weight and abdominal fat were significantly (p<.05) lower in the two groups as DC and DRE than the other groups as CON and RE. There was no significant difference in the weight of heart and skeletal muscle. Eight weeks of exercise or caloric reduction diet significantly (p<.05) reduced gene expression and activity of Traf2-NFkB-mTOR, which is a hypertrophic factor of cardiac muscle in obese middle-aged rats, and that autophagy factors of SIRT1-FoxO1 gene expression and activity were significantly (p<.05) increased. However, there was no synergistic effect with the combined treatment of the two stimuli. CONCLUSIONS: These results suggest that caloric reduction with weight loss or resistance training may be effective in preventing cardiac hypertrophy and heart failure in middle-aged obese rats.

참고문헌 (56)

  1. Eckel RH, "Report of the National Heart, Lung, and Blood Institute-National Institute of Diabetes and Digestive and Kidney Diseases Working Group on the pathophysiology of obesity-associated cardiovascular disease", Circulation, 105(24), pp.2923~2928 (2002)
  2. Levy D, "Prognostic implications of echocardio-graphically determined left ventricular mass in the Framingham Heart Study", N Eng J Med, 322(22), pp.1561~1566 (1990)
  3. Li SY, "Cardiac contractile dysfunction in Lep", Lep obesity is accompanied by NADPH oxidase activation, oxidative modification of sarco(endo)plasmic reticulum Ca2+-ATPase and myosin heavy chain isozyme switch, 2006, pp.49(6), 1434~1446
  4. Sowers JR, "Obesity as a cardiovascular risk factor", Am J Med, 115, pp.37~41 (2003)
  5. Feuvray D, "Diabetes-related metabolic perturbations in cardiac myocyte", Diabetes Metab, 34, pp.3~9 (2008)
  6. Boudina S, "Mitochondrial uncoupling : a key contributor to reduced cardiac efficiency in diabetes", Physiology(Bethesda), 21, pp.250~258 (2006)
  7. Dong F, "Metallothionein prevents high-fat diet induced cardiac contractile dysfunction : role of peroxisome proliferator activated receptor gamma coactivator1alpha and mitochondrial biogenesis", Diabetes, 56(9), pp.2201~2212 (2007)
  8. Lima-Leopoldo AP, "Obesity induces upregulation of genes involved in myocardial Ca2+ handling", Braz J Med Biol Res, 41(7), pp.615~620 (2008)
  9. Ouwens DM, "Cardiac dysfunction induced by high-fat diet is associated with altered myocardial insulin signalling in rats", Diabetologia, 48(6), pp.1229~1237 (2005)
  10. Smeets PJ, "Inflammatory pathways are activated during cardiomyocyte hypertrophy and attenuated by peroxisome proliferator-activated receptors PPARalpha and PPARdelta", J Biol Chem, 283(43), pp.29109~29118 (2008)
  11. Thaik CM, "Interleukin-1 beta modulates the growth and phenotype of neonatal rat cardiac myocytes", J Clin Invest, 96(2), pp.1093~1099 (1995)
  12. Yokoyama T, "Tumor necrosis factor-alpha provokes a hypertrophic growth response in adult cardiac myocytes", Circulation, 95(5), pp.1247~1252 (1997)
  13. Jang KW, "Ubiquitin ligase CHIP induces TRAF2 proteasomal degradation and NF-kappaB inactivation to regulate breast cancer cell invasion", J Cell Biochem, 112(12), pp.3612~3620 (2011)
  14. Zhang X, "TRAF2 regulates the cytoplasmic", nuclear distribution of TRAF4 and its biological function in breast cancer cells, 2013, pp.436(2), 344~348
  15. Burchfield JS, "The cytoprotective effects of tumor necrosis factor are conveyed through tumor necrosis factor receptor-associated factor 2 in the heart", Circ Heart Fail, 3(1), pp.157~164 (2010)
  16. Divakaran VG, "Tumor necrosis factor receptor-associated factor 2 signaling provokes adverse cardiac remodeling in the adult mammalian heart", Circ Heart Fail, 6(3), pp.535~543 (2013)
  17. Wang CY, "Obesity increases vascular senescence and susceptibility to ischemic injury through chronic activation of Akt and mTOR", Sci Signal, 2(62), pp.ra11~ (2009)
  18. Sciarretta S, "Markers of inflammation and fibrosis are related to cardiovascular damage in hypertensive patients with metabolic syndrome", Am J Hypertens, 20(7), pp.784~791 (2007)
  19. Sciarretta S, "Rheb is a critical regulator of autophagy during myocardial ischemia : pathophysiological implications in obesity and metabolic syndrome", Circulation, 125(9), pp.1134~1146 (2012)
  20. Sengupta A, "FoxO transcription factors promote autophagy in cardiomyocytes", J Biol Chem, 284(41), pp.28319~28331 (2009)
  21. Gustafsson AB, "Recycle or die : the role of autophagy in cardioprotection", J Mol Cell Cardiol, 44(4), pp.654~661 (2008)
  22. Nishida K, "The role of autophagy in the heart", Cell Death Differ, 16(1), pp.31~38 (2009)
  23. Mammucari C, "FoxO3controls autophagy in skeletal muscle in vivo", Cell Metab, 6(6), pp.458~471 (2007)
  24. Zhao J, "FoxO3 coordinately activates protein degradation by the autophagic", lysosomal and proteasomal pathways in atrophying muscle cells, 2007, pp.6(6), 472~483
  25. Blinderman CD, "Symptom distress and quality of life in patients with advanced congestive heart failure", J Pain Symptom Manage, 35(6), pp.594~603 (2008)
  26. Cowie MR, "Survival of patients with a new diagnosis of heart failure : a population based study", Heart, 83(5), pp.505~510 (2000)
  27. Bleumink GS, "Quantifying the heart failure epidemic : prevalence, incidence rate, lifetime risk and prognosis of heart failure The Rotterdam Study", Eur Heart J, 25(18), pp.1614~1619 (2004)
  28. Pluim BM, "The athlete’s heart. A meta-analysis of cardiac structure and function", Circulation, 101(3), pp.336~344 (2000)
  29. Eghbali M, "Molecular and functional signature of heart hypertrophy during pregnancy", Circ Res, 96(11), pp.1208~1216 (2005)
  30. Zak R, "Cardiac hypertrophy : its characteristics as a growth process", Am J Cardiol, 44(5), pp.941~946 (1979)
  31. Barboza CA, "Cardioprotective properties of aerobic and resistance training against myocardial infarction", Int J Sports Med, 37(6), pp.421~430 (2016)
  32. Jung S, "The effect of ladder-climbing exercise on atrophy", hypertrophy-related myokine expression in middle-aged male Wistar rats, 2015, pp.65(6), 515~521
  33. Kim JY, "High-fat diet- induced muscle insulin resistance: relationship to visceral fat mass", Am J Physiol Regul Integr Comp Physiol, 279(6), pp.R2057~2065 (2000)
  34. Lowry OH, "Protein measurement with the Folin phenol reagent", J Biol Chem, 193(1), pp.265~275 (1951)
  35. Yusuf S, "Obesity and the risk of myocardial infarction in 27, 000 participants from 52countries : a case-control study", Lancet, 366(9497), pp.1640~1649 (2005)
  36. Bouhidel O, "Myocardial ischemic postconditioning against ischemia-reperfusion is impaired in ob", ob mice, 2008, pp.295(4), H1580~1586
  37. Ferdinandy P, "Interaction of cardiovascular risk factors with myocardial ischemia", reperfusion injury, preconditioning and post-conditioning, 2007, pp.59(4), 418~458
  38. Peart JN, "Clinical cardioprotection and the value of conditioning responses", American journal of physiology. Am J Physiol Heart Circ Physiol, 296(6), pp.H1705~1720 (2009)
  39. Huang Y, "Cardiac-specific Traf2overexpression enhances cardiac hypertrophy through activating AKT", GSK3β signaling, 2014, pp.536(2), 225~231
  40. Ermolaeva MA, "Function of TRADD in tumor necrosis factor receptor1signaling and in TRIF-dependent inflammatory responses", Nature Immunol, 9(9), pp.1037~1046 (2008)
  41. Jono H, "NF-kappaB is essential for induction of CYLD, the negative regulator of NF-kappaB : evidence for a novel inducible autoregulatory feedback pathway", J Biol Chem, 279(35), pp.36171~36174 (2004)
  42. Tsang CK, "Targeting mammalian target of rapamycin(mTOR) for health and diseases", Drug Discov Today, 12(3-4), pp.112~124 (2007)
  43. Wang C, "Rapamycin-treated human endothelial cells preferentially activate allogeneic regulatory T cells", J Clin Invest, 123(4), pp.1677~1693 (2013)
  44. Blair SN, "Effects of physical inactivity and obesity on morbidity and mortality: current evidence and research issues", Med Sci Sports Exerc, 31(11), pp.S646~662 (1999)
  45. Savage PD, "Reduction in obesity and coronary risk factors after high caloric exercise training in overweight coronary patients", Am Heart J, 146(2), pp.317~323 (2003)
  46. Golbidi S, "Molecular mechanisms in exercise-induced cardioprotection", Cardiol Res Pract, 2011, pp.972807~ (2011)
  47. Cunningham JT, "mTOR controls mitochondrial oxidative function through a YY1-PGC-1α transcriptional complex", Nature, 450(7170), pp.736~740 (2007)
  48. Chong ZZ, "Cardiovascular disease and mTOR signaling", Trends Cardiovasc Med, 21(5), pp.151~155 (2011)
  49. Wagner C, "Ischemic post-conditioning reduces infarct size of the in vivo rat heart:role of PI3-K, mTOR, GSK-3β, and apoptosis", Mol Cell Biochem, 339(1-2), pp.135~147 (2010)
  50. Pal R, "Src-dependent impairment of autophagy by oxidative stress in a mouse model of Duchenne muscular dystrophy", Nat Commun, 5, pp.4425~ (2014)
  51. Jung CH, "ULK-Atg13-FIP200complexes mediate mTOR signaling to the autophagy machinery", Mol Biol Cell, 20(7), pp.1992~2003 (2009)
  52. Qu H, "1, 25(OH)2D3 improves cardiac dysfunction, hypertrophy, and fibrosis through PARP1", SIRT1, Mol Nutr Food Res, pp.2017, 61(5)[DOI]
  53. Xie X, "IkappaB kinase epsilon and TANK-binding kinase 1 activate AKT by direct phosphorylation", Proc Natl Acad Sci USA, 108(16), pp.6474~6479 (2011)
  54. Kaipeng Jing, "Why is autophagy important in human diseases?", Experimental and Molecular Medicine, 44(2), pp.69~72 (2012), 생화학분자생물학회
  55. Wang Y, "Autophagy regulates inflammation following oxidative injury in diabetes", Autophagy, 9(3), pp.272~277 (2013)
  56. DeBosch B, "Akt2 regulates cardiac metabolism and cardiomyocyte survival", J Biol Chem, 281(43), pp.32841~32851 (2006)

관련 논문AI추천

이 간행물의 다른 논문 (9건)