운동 강도가 백혈구 텔로미어, 지질과산화 및 항산화 효소 활성도에 미치는 영향
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전태원, 신윤아. 운동 강도가 백혈구 텔로미어, 지질과산화 및 항산화 효소 활성도에 미치는 영향. 운동과학, 제16권 제2호. 75-84, 2007. 본 연구의 목적은 중고강도의 운동이 백혈구 텔로미어 길이와 지질과산화 및 항산화효소의 활성도에 미치는 영향을 규명하는 것이다. 본 연구는 운동경험이 없는 폐경 전 여성(46.81±6.43세)으로 10명을 대상으로 하였다. 피검자들은 60%VO2max 및 80%VO2max 강도에서 트레드밀 달리기를 실시하였으며, 운동시간에 따른 에너지 소비량은 400kcal로 고정하여 개인별로 측정하였다. 채혈은 안정시, 운동 직후에 전완주정맥에서 20ml 채혈하였으며, 지질과산화물과 항산화 효소 및 텔로미어 DNA의 변화 양상을 분석하였다. 이상의 연구절차를 통해 얻은 연구결과는 다음과 같다. 운동 강도에 따른 백혈구 텔로미어 길이는 60%VO2max 및 80%VO2max 운동 직후 모두 유의하게 감소하였으며(p<.01), 지질과산화는 운동 직후 유의하게 증가하였고(p<.01), SOD 활성도는 운동 직후 유의하게 감소하였다(p<.01). 백혈구 텔로미어, MDA, SOD와 GPx모두 운동 강도 및 시간과 운동 강도의 상호작용 효과는 통계적으로 유의한 차이를 나타내지 않았다(p>.05). 이상의 결과로 중고강도의 400kcal를 소비하는 운동은 운동경험이 없는 중년여성들에게 산화 스트레스를 증가시키고 항산화 효소의 활성도를 감소시켜 백혈구 텔로미어 길이에 영향을 준 것으로 생각된다. 또한 면역계의 memory T cell도 운동 후 백혈구 텔로미어 길이의 감소에 영향을 미친 것으로 사료된다.
Abstract
Jun, T.W., & Shin, Y.A. The influence of exercise intensity on telomere length in white blood cell, MDA, and antioxidant enzyme activity. Exercise Science, 16(2): 75-84, 2007. The purpose of this study was to investigate the changes in mean TRF length in white blood cell, the oxidative stress and antioxidants enzymes activities in response to moderate and high exercise intensities. Ten sedentary healthy middle aged women(46.81±6.43 years old)participated in this study. They performed 60% and 80% VO2max treadmill exercise. The exercise duration was adjusted so that the energy expenditure was equal in all subjects(400kcal). Blood sample of 20ml was drawn from antecubital veins for measuring telomere length, MDA, SOD, and GPx before and after exercise test. The results can be summarized as follows. The mean TRF length was significantly reduced immediately after exercise in both 60% and 80% VO2max. Moreover, plasma MDA level was significantly increased, and SOD activity was significantly decreased after exercise. There was no difference between 60% and 80% VO2max, in terms of mean TRF length, MDA, SOD, and GPx activity. These findings suggest that physical stress from energy expenditure over 400kcal is significantly associated with higher oxidative stress, lower antioxidant enzymes activities and shortening telomere length in peripheral white blood cells. Also, activated T cells with shortened telomeres mobilized from peripheral reservoirs might have some influence over telomere shortening.* 이 연구는 2005년 정부재원(교육인적자원부 학술연구조성사업)으로 한국학술진흥재단의 선도연구지원을 받아 연구되었음(KRF-2005-041-G00154)
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