장기간 중강도 운동 훈련이 쥐 대식세포의 산화질소 및 싸이토카인 발현에 미치는 영향
Effect of long-term moderate exercise training on macrophage nitric oxide and cytokine production in mice.
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본 연구의 목적은 장기간의 중강도 운동 훈련이 마우스 대식세포의 산화질소, 싸이토카인 발현량, 식균작용에 미치는 영향을 규명하는 것이다. 실험동물로써 4주령의 BALB/c 마우스 20마리를 사용하였으며, 1주간의 적응기 후에 무작위로 통제군 10마리(Con, n=10)와 운동군 10마리(Exe, n=10)를 분류하였다. 운동은 쥐용 트레드밀을 이용하였으며, 운동훈련은 16주간 중강도(8-14m/min, 40~50분/일, 주 5회)로 실시하였다. 16주간의 운동 후 마우스 복강에서 추출한 대식세포를 대상으로 외부자극물질(LPS 1㎍/㎖, LPS 0.5㎍/㎖+IFN-γ 100 Unit)로 자극한 후 산화질소, 싸이토카인(IL-12, IFN-γ)발현량과 식균작용을 측정하였다. 통계적 방법으로는 독립 t-검증을 실시하였으며, 유의수준은 p<.05로 설정하여 다음과 같은 결과를 얻었다. 첫째 장기간 중강도 운동훈련은 마우스 대식세포에 있어 LPS + IFN-γ 동시 처치 후 IL-12 발현량을 증가시켰다. 둘째 장기간 중강도 운동훈련은 마우스 대식세포의 식균작용을 향상시키는 것으로 나타났다. 결론적으로 장기간 중강도 운동 훈련은 선천면역에 있어 중요한 역할을 담당하는 대식세포의 식균작용 향상과 싸이토카인 분비를 증가시키는 것으로 나타나 선천면역기능 향상에 긍정적 역할을 하는 것으로 사료된다.
Abstract
The purpose of this study was to analyze the effect of long-term moderate exercise training on macrophage immunoresponse in mice. To accomplish the purpose of this study, mice were divided into two groups: a nonexercise group(control, n=10) and exercise group (exercise, n=10). The exercise applied was 5days/wk treadmill running (without electrical shock treatment) at 10-14m/min (60-76% of maximal oxygen uptake) on flat floor without slope for 16wk. Nitric oxide, IL-12, IFN-γ production, Phagocytosis were measured. The results were as followings; First, IL-12 production by LPS+IFN-γ stimulated peritoneal macrophages from the exercise group was significantly higher than that in the control group. Second, Phagocytic index was significantly higher than that in the control group. These result suggest that long-term moderate exercise training enhances the phagocytosis by improving the function of the peritoneal macrophages. In conclusion it seemed that long-term moderate exercise training could positive effect on macrophage immunoresponse by improving IL-12 production which helps Th1 cells. However, Nitric Oxide and IL-1ß, IFN-γ were not significantly changed by long-term moderate exercise training.
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