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장기간 중강도 운동훈련과 일회 탈진운동이 쥐 비장세포의 싸이토카인 생성량 및 증식반응에 미치는 영향

Effects of acute exhaustive exercise and long-term moderate exercise training on splenocyte immunocompetence in mice

이왕록|박희근|김명화|윤아람|정준현|권순미|전종귀
한국운동생리학회|2009.11|운동과학 (Exercise Science)|제18권 제4호|pp. 467-474|DOI 10.15857/ksep.2009.18.4.467

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참고문헌

키워드

cytokine싸이토카인spleenproliferation탈진운동exhaustive exercise중강도 운동훈련비장세포증식반응moderate exercise training

초록

본 연구의 목적은 장기간 중강도 운동훈련과 일회 탈진운동이 쥐 비장세포의 면역반응에 미치는 영향을 규명하는 것이다. 실험동물로써 4주령의 BALB/c 마우스 30마리를 사용하였으며, 1주간의 적응기 후에 무작위로 통제군(n=10), 탈진운동군(n=10), 장기간 중강도 운동군(n=10)으로 분류하였다. 운동은 쥐용 트레드밀을 이용하였으며, 운동훈련은 탈진운동(14-28m/min, 5%, ～90분, 16주), 장기간 중강도 운동(10-14m/min, 30-50min, 주5회, 16주간)을 실시하였다. 16주간 운동 후 비장세포를 추출하여 다양한 농도의 concanavalin A로 자극한 후 IL-1β, IL-4, IL-12, IFN-γ과 증식반응을 측정하였다. 통계적 방법으로는 일원변량분석을 실시하였으며, 유의 수준은 α=.05로 설정하여 다음과 같은 결과를 얻었다. 탈진운동군에서 IL-12의 생성량이 다른 두 그룹보다 유의하게 높았지만, IL-1β, IL-4, IFN-γ와 증식반응에서는 유의한 차이가 나타나지 않아 비장세포 있어 탈진운동이 면역반응을 저하시키거나 중강도 운동이 향상시키는 결과는 얻지 못하였다. 결론적으로 마우스 비장세포에서 Th1 계열의 IL-12 생성량 향상에 영향미치는 것으로 나타나 탈진운동이 세포매개성 면역반응에 영향을 주는 것으로 보여지며 운동강도에 따라 면역세포간 각기 다른 반응을 나타내는 것으로 사료된다.

Abstract

The present research was to investigate the effect of exhaustive exercise and long-term moderate exercise training on splenocyte immunoresponse in mice. Male Balb/c mice (4 weeks, n = 30) were used and randomly divided into 3 groups; Control group (CON, n=10), Exhaustive exercise group (EXH, n=10, 14-28 m/min, 5%, ≒90 min at 16 weeks) and Moderate exercise training group (TRA, n=10, 10-14 m/min, 30-50 min/day, 5 day/week for 16 weeks). After exercise training, all the splenocyte was collected and multiful doses of Con A(Concanavalin A) were used to stimulate the cells. Then, IL-1β, IL-4, IL-12, IFN-γ and cell proliferation were measured by commercial ELISA kits. One-way ANOVA were used and p value under 0.05 was considered as statistical significance. IL-12 production of exhaustive exercise was higher than other groups. However, IL-1β, IL-4, IFN-γ and cell proliferation and were not significantly affected by both exhaustive exercise and a long-term moderate exercise training. In conclusion, exhaustive exercise could enhance to produce IL-12 cytokine(Th1 cytokine) in splenocyte, which help cell-mediated immunity. Further exercise-induced immunocompetence would be different in each immune cells.

참고문헌 (39)

  1. 곽이섭, "운동에 따른 비장 및 복강 활성산소의 변화가 비장 림프구 증식에 미치는 영향", 체육과학연구, 14(1), pp.1~8 (2003), 국민체육진흥공단 부설 한국스포츠개발원
  2. 곽이섭, "수영 트레이닝이 마우스의 림프계에 미치는 영향", 한국체육학회지, 41(2), pp.535~542 (2002), 한국체육학회
  3. Blair, S. N., "Is physical activity or physical fittness more important in defining health benefits?", Med. Sci. Sports Exerc, 33(6), pp.379~399 (2001)
  4. Ceddia, M. A., "Exercise suppresses macrophage antigen presentation", J. Appl. Physiol, 87(6), pp.2253~2258 (1999)
  5. Concordet J. P., "Physiological programmed cell death in thymocytes is induced by physical stress(exercise)", Am J Physiol, 265, pp.626~629 (1993)
  6. Cooper, H. L., "Studies on RNA metabolism during lymphocyte activation", Transplant Rev, 11(3) (1972)
  7. Davis, J. M., "Exercise, alveolar macrophage, function and susceptibility to respiratory infection", J.Appl.Physiol, 83, pp.1461~1466 (1997)
  8. Eskandari, F., "Neural-immune interactions in health and disease", Ann.N Y Acad.Sci, 966, pp.20~27 (2002)
  9. Gleeson, M., "Exercise, nutrition and immune function", J Sports Sci, 22(1), pp.115~125 (2004)
  10. Hartmann, A., "Does physical activity induce DNA damage?", Mutagenesis, 9, pp.269~272 (1994)
  11. Hinton, J. R., "Acute intensive interval training and in vitro T-lymphocyte function", Int J Sports Med, 18, pp.130~135 (1997)
  12. Hoffman-Goetz, L., "Effect of estradiol and execise on lymphocyte proliferation response in female mice", Physiology & Behavior, 68, pp.169~174 (1999)
  13. Jonsdottir, I. H., "Duration and mechanisms of the increased natural cytotoxicity seen after chronic voluntary exercise in rats", Acta Physiol Scand, 160, pp.333~339 (1997)
  14. Kapasi, Z. F., "Suppression of viral specific primary T-cell response following intense physical exercise in young but not old mice", J. Appl. Physiol, 98(2), pp.663~671 (2005)
  15. Katherine, J, "Acute Exercise and T-Lymphocyte Expression of the Early Activation Marker CD69", Medcine Science in Sports & Exercise, ~ (2003)
  16. Kohut, M. L., "Exercise training-induced adaptations of immune response are mediated by β-adrenergic receptors in aged but not young mice", J Appl Physiol, 96, pp.1312~1322 (2004)
  17. Kohut, M. L., "Moderate exercise is associated with enhanced antigen-specific cytokine, but not lgM production in aged mice", Mech Ageing Dev, 122, pp.1135~1150 (2001)
  18. Kohut, M. L., "Exercise and psychosocial factor modulate immunity to influenza vaccine in elderly individuals", J Gerontol A Biol Sci Med Sci, 57, pp.557~562 (2002)
  19. Lakka, T. A., "Effect of exercise training on plasma levels of C reactive protein in healthy adults : the HERITAGE family study", Eur Heart J, 26, pp.2018~2025 (2005)
  20. Mackinnon, L. T., "Future directions in exercise and immunology:regulation and integration", Int J Sports Med, 19, pp.205~211 (1998)
  21. Madden, K. S., "Catecholamines, sympathetic innervation, and immunity. Brain Behav", Immun, 17, pp.5~10 (2003)
  22. Melzer, K., "Physical activity:the health benefits outweigh the risks", Curr. Opin.Clin. Nutr. Metab. Care, 7(6), pp.641~647 (2004)
  23. Miller, R. A., "The aging immune system: primer and prospectus", Science, 273, pp.70~73 (1996)
  24. Nehlsen-Cannarella, S. L., "The effect of moderate exercise training on immune respones", Med. Sci. Sports Exercise, 23(1), pp.64~70 (1991)
  25. Nielsen, H. B., "Lymphocyte response to maximal exercise:a physiological perspective", Sports Medicine, 18, pp.91~100 (2003)
  26. Nieman, D. C., "Physical activity and immune function in elderly women", Med Sci Sports Exeec, 25, pp.823~831 (1993)
  27. Nieman, D. C., "Special feature for the Olympic: effects of exercise on the immune system: exercise effects on systemic immunity", Immunol Cell Biol, 78, pp.496~501 (2000)
  28. Peijie, C. L., "Heavy load exercise induced dysfunction of immunity and neuroendocrine response in rat", Life Science, 72, pp.2255~2262 (2003)
  29. Pedersen, B. K., "Exercise and interleukin-6", Curr Opin Hematol, 8, pp.137~141 (2001)
  30. Penkowa, M., "Immunohistochemical detection of interleukin-6 in human skeletal muscle fibers following exercise", FASEB J, 17, pp.2166~2168 (2003)
  31. Ronald, V. T. S., "Effects of acute exhaustive physical exercise upon glutamine metabolism of lymphocytes from trained rats", Life sciences, 80, pp.573~578 (2007)
  32. Schefer, V., "consumption in adult and aged C57BL", 6J mice during acute treadmill exercise of different intensity, 1996, pp.31, 387~392
  33. Shinkai, S., "Physical activity and immune senscence in men", Med Sci Sports Exerc, 27, pp.1516~1526 (1995)
  34. Singh, S. K., "Immuno-modulation by exercise review", Indian.J.Med.Sci.May, 46(5), pp.158~160 (1992)
  35. Sugiura, H., "Immunomodulation by 8-week voluntary exercise in mice", Acta Physiologica Scandinavica, 168, pp.413~420 (2000)
  36. Sugiura, H., "Immunomodulatory action of chronic exercise on macrophage and lymphocyte cytokine production in mice", Acta Physiol Scand, 174(3), pp.247~256 (2002)
  37. Sugiura, H., "Effects of different frequency of exercise on macrophage function and lymphocyte proliferation in mice", Adv Exerc Sports Physiol, 4, pp.93~102 (1998)
  38. Weinstock, C., "Effect of exhaustive exercise stress on the cytokine response", Medicine & Science in Sports & Exercise, 29(3), pp.345~354 (1997)
  39. Woods, J. A., "Exercise and cellular innate immune function", Med.Sci.Sports Exerc, 31, pp.57~66 (1999)

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