본문 바로가기
KERD
논문KCI 등재

고지방식이 비만쥐 모델에서 중강도 운동 훈련이 지방대사 및 대식세포 침윤에 미치는 영향

Moderate exercise training inhibits lipid metabolism and macrophage infiltration in high fat diet-induced obese mice

1

조회

0

다운로드

4

인용

0

참고문헌

키워드

초록

본 연구는 고지방식이 쥐를 대상으로 중강도 운동 실시가 지방조직에서 대식세포 침윤과 지방조직 리모델링의 억제효과를 살펴보고자 하였다. C57BL/6 수컷 마우스를 HD 그룹(고지방식이 통제군, n=10)과 HE 그룹(고지방식이 운동군, n=10) 분류한 후 12주간 트레드밀 운동(40-60min/day, 10-22m/min, 5days/week)을 실시하였다. 본 연구 결과 HE 그룹이 체중과 부고환지방조직 무게에서 HD 그룹보다 유의한 감소를 보였고(p<.05), 부고환지방조직이 지방세포 크기에서도 HE 그룹이 HD 그룹보다 유의하게 감소하였다(p<.05). 또한 지방조직내 대식세포 침윤을 분석한 결과 CD11c, F480, CD86 mRNA 발현량에서 HE 그룹이 HD 그룹보다 유의하게 감소하였고(p<.05), 지방합성과 관련된 aP2 mRNA 발현에서도 HE 그룹이 HD 그룹보다 유의한 감소를 보였다(p<.05). 본 연구결과 고지방식이로 유도된 비만모델에서 중강도 운동은 지방세포 크기의 감소를 통하여 지방조직 감소에 긍정적인 영향을 미치며, 지방량증가로 인한 지방조직내 대식세포 침윤을 억제하는데 유익한 영향을 미친다고 사료된다.

Abstract

This study was investigated the effect of moderate exercise training on adipose tissue remodeling and macrophage infiltration in high fat diet induced obese mice. To accomplish the purpose of this study, C57BL/6 male mice were fed high fat diet (45% fat diet) during experimental period. The animals were divided into 2 groups ; HD(high fat diet control, n=10), and HE(high fat diet moderate exercise training, n=10). Exercise training was performed for 12 weeks on a treadmill running for 40-60min/day at 10-22m/min, 0% grade, 5 days/week. As a result, body weight and epididymal fat pad weight were significantly decreased in HE compared with HD (p<.05). Also, the size of adipose was significantly reduced in HE compared with HD (p<.05). Macrophage infiltration markers (CD11c, F480, CD86) mRNA expression were significantly decreased in HE compared to HD (p<.05). Moreover, adipogenesis marker (aP2) mRNA expression was significantly decreased in HE compared to HD (p<.05). These findings suggest that moderate exercise training has beneficial effects to inhibit adipose tissue remodeling and macrophage infiltration in high fat diet-induced obese mice.

참고문헌 (35)

  1. Bradley RL, "Voluntary exercise improves insulin sensitivity and adipose tissue inflammation in diet-induced obese mice", American Journal of Physiology-Endocrinology and Metabolism, 295(3), pp.586~594 (2008)
  2. Bruun JM, "Diet and exercise reduce low-grade inflammation and macrophage infiltration in adipose tissue but not in skeletal muscle in severely obese subjects", American Journal of Physiology-Endocrinology and Metabolism, 290(5), pp.961~967 (2006)
  3. Choi KM, "Effect of exercise training on A-FABP, lipocalin-2 and RBP4 levels in obese women", Clinical Endocrinology, 70(4), pp.569~574 (2009)
  4. Cinti S, "Adipocyte death defines macrophage localization and function in adipose tissue of obese mice and humans", Journal of lipid research, 46(11), pp.2347~2355 (2005)
  5. Dandona P, "Inflammation:the link between insulin resistance, obesity and diabetes", Trends in immunology, 25(1), pp.4~7 (2004)
  6. Hu FB, "Adiposity as compared with physical activity in predicting mortality among women", New England Journal of Medicine, 351(26), pp.2694~2703 (2004)
  7. JJantsch J, "Hypoxia and hypoxia-inducible factor-1α modulate lipopolysaccharide-induced dendritic cell activation and function", The Journal of Immunology, 180(7), pp.4697~4705 (2008)
  8. 정준현, "Effect of moderate exercise training and low-fat diet on peritoneal macrophage immunocompetence in high-fat diet-induced obese mice model", Journal of Exercise Nutrition & Biochemistry, 16(3), pp.133~142 (2012), 한국운동영양학회
  9. Jiao P, "Obesity related upregulation of monocyte chemotactic factors in adipocyte:involvement of nuclear factor-kappa B and c-Jun NH2-terminal kinase pathways", Diabetes, 58, pp.104~115 (2009)
  10. Kanda H, "MCP-1 contributes to macrophage infiltration into adipose tissue, insulin resistance, and hepatic steatosis in obesity", Journal of Clinical Investigation, 116(6), pp.1494~1505 (2006)
  11. Kruger K, "Immune and Inflammatory Signaling Pathways in Exercise and Obesity", American Journal of Lifestyle Medicine (2014)
  12. Kawanishi N, "Exercise training inhibits inflammation in adipose tissue via both suppression of macrophage infiltration and acceleration of phenotypic switching from M1 to M2 macrophages in highfat-dietinduced obese mice", Exerc Immunol Rev, 16(16), pp.105~118 (2010)
  13. Kissebah AH, "Regional adiposity and morbidity", Physiological Reviews, 74(4), pp.761~811 (1994)
  14. Lumeng CN, "Obesity induces a phenotypic switch in adipose tissue macrophage polarization", Journal of Clinical Investigation, 117(1), pp.175~184 (2007)
  15. Furuhashi M, "Treatment of diabetes and atherosclerosis by inhibiting fatty-acid-binding protein aP2", Nature, 447(7147), pp.959~965 (2007)
  16. Maurige P, "Regional differences in adipose tissue metabolism between sedentary and endurance-trained women", The American journal of physiology, 273(3 pt 1), pp.497~506 (1997)
  17. Morifuji M, "Dietry soya protein intake and exercise training have an additive effect on skeletal muscle fatty acid oxidation enzyme activity and mRNA levels in rats", British journal of nutrition, 96(3), pp.469~475 (2006)
  18. Moro C, "Sex differences in lipolysis-regulating mechanisms in overweight subjects: effect of exercise intensity", Obesity, 15(9), pp.2245~2255 (2007)
  19. Nishmura S, "In vivo imaging in mice reveals local cell dynamics and inflammation in obese adipose tissue", The Journal of clinical investigation, 118(2), pp.710~721 (2008)
  20. Tsuguhito Ota, "Chemokine Systems Link Obesity to Insulin Resistance", Diabetes and Metabolism Journal, 37(3), pp.165~172 (2013), 대한당뇨병학회[DOI]
  21. Pang C, "Macrophage infiltration into adipose tissue may promote angiogenesis for adipose tissue remodeling in obesity", American Journal of Physiology-Endocrinology and Metabolism, 295(2), pp.313~322 (2008)
  22. Pillaed F, "Lipid oxidation on overweight men after exercise and food intake", Metabolism, 59(2), pp.267~274 (2010)
  23. Satoko M, "Effect of exercise training on adipocyte-size-dependent expression of leptin and adiponectin", Life Science, 86(17), pp.691~698 (2010)
  24. Schefer V, "Oxygen consumption in adult and aged C57BL", 6J mice during acute treadmill exercise of different intensity, 1996, pp.31(3), 387~392
  25. Shapiro ME, "Effects of Treatment of C57BL", 6j Mice Fed High vs. Low Fat Diets with Metformin or Rosiglitazone on Adiposity, Food Intake, Hyperglycemia and Insulin Resistance, 2001, ~
  26. Shiho F, "Regulatory Mechanisms for Adipose Tissue M1 and M2 Macrophages in Diet-Induced Obese Mice", Diabetes, 58(11), pp.2574~2582 (2009)
  27. Shinkai S, "Physical activity and immune senescence in men", Medicine and science in sports and exercise, 27(11), pp.1516~1526 (1995)
  28. Spalding KL, "Dynamics of fat cell turnover in humans", Nature, 453(7196), pp.783~787 (2008)
  29. Spriet LL, "New insights into the interaction of carbohydrate and fat metabolism during exercise", Sports Medicine, 44(1), pp.87~96 (2014)
  30. Strissel KJ, "Adipocyte death, adipose tissue remodeling, and obesity complications", Diabetes, 56(2), pp.2910~2918 (2007)
  31. Takayoshi S, "Adipose tissue macrophages:their role in adipose tissue remodeling", Journal of Leukocyte Biology, 88(1), pp.33~39 (2010)
  32. Weisberg S P, "CCR2 modulates inflammatory and metabolic effects of high-fat feeding", Journal of Clinical Investigation, 116(1), pp.115~124 (2006)
  33. Weissman C, "Nutrition in the intensive care unit", Critical Care, 3(5), pp.67~75 (1999)
  34. Xu H, "Chronic inflammation in fat plays a crucial role in the development of obesity-related insulin resistance", Journal of Clinical Investigation, 112(12), pp.1821~1830 (2003)
  35. Zachwieja JJ, "Exercise as treatment for obesity", Endocrinology and metabolism clinics of North America, 25(4), pp.965~988 (1996)

관련 논문AI추천

이 간행물의 다른 논문 (11건)