중강도 운동과 라스베라트롤 투여가 고지방식이 비만쥐 지방조직의 대식세포 침윤과 염증반응에 미치는 영향
Effects of Moderate Exercise Training and Resveratrol Supplementation on Macrophage Infiltration and Inflammation in Adipose Tissue of Obese Mice
1
조회
0
다운로드
2
인용
0
참고문헌
키워드
초록
PURPOSE: The aim of this study was to compare the effectiveness either resveratrol or exercise with a low fat diet on visceral and subcutaneous adipose tissue of high fat diet-induced obese mice. METHODS: C57BL/6 mice were fed a high fat diet for 12 weeks first and a low fat diet for 8 weeks later. Mice were divided into 5 groups: high-fat diet (HD), low-fat diet control (HLC), low-fat diet with resveratrol (HLR), low-fat diet with exercise (HLE), low-fat diet with exercise plus resveratrol (HLER). The training groups ran on a treadmill at 12-20 m/min for 30-60 min/day, 5 times/weeks for 8 week. After the 20 weeks, visceral and subcutaneous adipose tissues were analyzed. RESULTS: As a result, F480 mRNA expression of HLR, HLE, and HLER was significantly decreased compared to HD in both adipose tissues. CD11c mRNA expression was significantly decreased HLE, HLER in both adipose tissue. CD163 mRNA expression was significantly increased by HLE, HLER in visceral adipose tissue only. Exercise groups were markedly inhibited TLR4 mRNA expression, ICAM-1, VCAM-1 and IL-6, TNF-α, MCP-1 mRNA in visceral adipose tissue only. In visceral and subcutaneous adipose tissue, IL-6, TNF-α mRNA were partially prevented. CONCLUSIONS: These results suggest that exercise or resveratrol with a low-fat diet have a positive effect to ameliorate the obeseinduced metabolic disturbances compared to a low-fat diet alone. However, it seems that exercise with a low fat diet is more effectively macrophage infiltration in visceral than subcutaneous adipose tissue.
Abstract
PURPOSE: The aim of this study was to compare the effectiveness either resveratrol or exercise with a low fat diet on visceral and subcutaneous adipose tissue of high fat diet-induced obese mice. METHODS: C57BL/6 mice were fed a high fat diet for 12 weeks first and a low fat diet for 8 weeks later. Mice were divided into 5 groups: high-fat diet (HD), low-fat diet control (HLC), low-fat diet with resveratrol (HLR), low-fat diet with exercise (HLE), low-fat diet with exercise plus resveratrol (HLER). The training groups ran on a treadmill at 12-20 m/min for 30-60 min/day, 5 times/weeks for 8 week. After the 20 weeks, visceral and subcutaneous adipose tissues were analyzed. RESULTS: As a result, F480 mRNA expression of HLR, HLE, and HLER was significantly decreased compared to HD in both adipose tissues. CD11c mRNA expression was significantly decreased HLE, HLER in both adipose tissue. CD163 mRNA expression was significantly increased by HLE, HLER in visceral adipose tissue only. Exercise groups were markedly inhibited TLR4 mRNA expression, ICAM-1, VCAM-1 and IL-6, TNF-α, MCP-1 mRNA in visceral adipose tissue only. In visceral and subcutaneous adipose tissue, IL-6, TNF-α mRNA were partially prevented. CONCLUSIONS: These results suggest that exercise or resveratrol with a low-fat diet have a positive effect to ameliorate the obeseinduced metabolic disturbances compared to a low-fat diet alone. However, it seems that exercise with a low fat diet is more effectively macrophage infiltration in visceral than subcutaneous adipose tissue.
참고문헌 (23)
- Weisberg SP, "Obesity is associated with macrophage accumulation in adipose tissue", Journal of Clinical Investigation, 112(12), pp.1796~1808 (2003)
- Lumeng CN, "Obesity induces a phenotypic switch in adipose tissue macrophage polarization", Journal of Clinical Investigation, 117(1), pp.175~184 (2007)
- Suganami T, "A Paracrine loop between adipocytes and macrophages aggravates inflammatory changes : role of free fatty acids and tumor necrosis factor alpha", Arteriosclerosis, Thrombosis, and Vascular Biology, 25(10), pp.2062~2068 (2005)
- Goodpaster BH, "Obesity, regional body fat distribution, and the metabolic syndrome in older men and women", Archives of Internal Medicine, 165(7), pp.777~783 (2005)
- Fujioka S, "Contribution of intraabdominal fat accumulation to the impairment of glucose and lipid metabolism in human obesity", Metabolism, 36(1), pp.54~59 (1987)
- Goodpaster BH, "Subcutaneous abdominal fat and thigh muscle composition predict insulin sensitivity independently of visceral fat", Diabetes, 46(10), pp.1579~1585 (1997)
- Lumeng CN, "Phenotypic switching of adipose tissue macrophages with obesity is generated by spatiotemporal differences in macrophage subtypes", Diabetes, 57(12), pp.3239~3246 (2008)
- Satoh N, "Unbalanced M1", M2 phenotype of peripheral blood monocytes in obese diabetic patients: effect of pioglitazone, 2010, pp.33(1), e7~e7
- 이영란, "지구성운동이 고혈압 동물의 지방조직 내 염증, 세포사멸 및 FOXO 발현에 미치는 효과", 운동과학, 23(2), pp.89~98 (2014), 한국운동생리학회
- 정준현, "고지방식이 비만쥐 모델에서 중강도 운동 훈련이 지방대사 및 대식세포 침윤에 미치는 영향", 운동과학, 24(2), pp.161~167 (2015), 한국운동생리학회[DOI]
- Erlund I, "Review of the flavinoids quercetin, hesperetin, and naringenin. Dietary sources, bio activities, bioavailability, and epidemiology", Nutrition Research, 24(10), pp.851~874 (2004)
- 이영란, "유산소 운동과 케르세틴 섭취 및 저지방 식이 복합 처치가 비만 쥐 골격근의 지질대사, 미토콘드리아 생합성 및 염증성 싸이토카인에 미치는 효과", 운동과학, 22(2), pp.113~123 (2013), 한국운동생리학회
- Kang L, "Resveratrol modulates adipokine expression and improves insulin sensitivity in adipocytes:Relative to inhibition of inflammatory responses", Biochimie, 92(7), pp.789~796 (2010)
- Schefer V, "Oxygen consumption in adult and aged C57BL", 6J mice during acute treadmill exercise of different intensity, 1996, pp.31(3), 387~392
- 이왕록, "장기간 중강도 운동 훈련이 쥐 대식세포의 산화질소 및 싸이토카인 발현에 미치는 영향", 운동과학, 18(2), pp.203~212 (2009), 한국운동생리학회
- 이왕록, "장기간 중강도 운동훈련과 일회 탈진운동이 쥐 비장세포의 싸이토카인 생성량 및 증식반응에 미치는 영향", 운동과학, 18(4), pp.467~474 (2009), 한국운동생리학회
- Lagouge M, "Resveratrol improves mitochondrial function and protects against metabolic disease by activating SIRT1 and PGC-1a", Cell, 127(6), pp.1109~1122 (2006)
- Baur JA, "Resveratrol improves health and survival of mice on high-calorie diet", Nature, 444(7117), pp.337~342 (2006)
- Juan ME, "The daily oral administration of high doses of trans-resveratrol to rats for 28 days is not harmful", Journal of Nutrition, 132(2), pp.257~260 (2002)
- Mayers JR, "Resveratrol treatment in mice does not elicit the bradycardia and hypothermia associated with calorie restriction", The FASEB Journal, 23(4), pp.1032~1040 (2009)
- Kanda H, "MCP-1 contributes to macrophage infiltration into adipose tissue, insulin resistance, and hepatic steatosis in obesity", The Journal of Clinical Investigation, 116(6), pp.1494~1505 (2006)
- Kawanishi N, "Exercise training inhibits inflammation in adipose tissue via both suppression of macrophage infiltration and acceleration of phenotypic switching from M1 to M2macrophages in high-fat-diet induced obese mice", Exercise Immunology Review, 16, pp.105~118 (2010)
- Karla MP, "Visceral and subcutaneous adipose tissue Volumes are cross-sectionally related to markers of inflammation and oxidative stress : the Framingham Heart Study", Circulation, 116(11), pp.1234~1241 (2007)