본문 바로가기
KERD
논문KCI 등재

트레드밀 운동이 고지방 식이 쥐의 해마에서의 소포체 스트레스와 인슐린 저항성에 미치는 영향

Effect of treadmill exercise on ER stress and insulin resistance in the hippocampus of high-fat diet fed rats

강은범
한국운동생리학회|2015.05|운동과학 (Exercise Science)|제24권 제2호|pp. 143-151|DOI 10.15857/ksep.2015.24.2.143

0

조회

0

다운로드

2

인용

0

참고문헌

키워드

비만obesity뇌braintreadmill exercise소포체 스트레스ER stress트레드밀 달리기

초록

목적이 연구의 목적은 20주간 고지방 식이를 통해 비만이 유도된 실험동물을 대상으로 8주간의 트레드밀 운동이 당대사, 인슐린저항성 그리고 뇌 해마조직에서 ER stress 관련 인자들에 미치는 영향을 규명하는 것이다. 방법20주령의 SD rat을 대상으로 비만을 유도하기 위해 고지방사료를 20주간 공급하였다. 실험동물은 정상식이 비교집단(ND-CON, n=6), 고지방식이 비교집단(HFD-CON, n=6), 고지방식이 운동집단(HFD-CON, n=6)으로 구분하였다. 트레드밀 운동은 하루 30분씩 주4일, 8주간 실시하였다. 트레드밀 운동이 끝난 후에 체중, AUC, HOMA-IR을 분석하였으며, Western blot 방법을 이용하여 ER stress 관련 변인(GRP78/BiP, PERK, ATF6, IRE1α, eIF2α, XBP-1S, CHOP)들을 분석하였다. 결과HFD-CON 집단은 ND-CON 집단에 비해 체중이 증가하고, 혈당처리능력이 감소되었으며, HOMA-IR이 증가된 것으로 나타났다. 또한 ER stress 관련 변인들도 발현이 증가된 것으로 나타나 세포사멸 신호전달 경로가 활성화된 것으로 나타났다. 하지만 트레드밀 운동을 실시한 HFD-EXE 집단은 HFD-CON 집단에 비해 체중이 감소되고, 혈당처리능력이 개선되었으며 HOMA-IR도 감소한 것으로 나타났다. ER stress 관련 변인들도 HFD-EXE 집단은 HFD-CON 집단에 비해 감소된 것으로 나타나 ER stress가 감소된 것으로 나타났다. 결론결과적으로 트레드밀 운동과 같은 유산소 운동은 비만으로 유발된 체내 에너지 대사 불균형을 개선하고 뇌 해마부위에서 유발된 ER stress를 억제하는데 긍정적인 역할을 하는 것으로 나타났다.

Abstract

PURPOSE: The purpose of this study was to investigate the impacts of 8-week treadmill exercise on glycose metabolism, insulin resistance and ER stress-related factors in hippocampal tissue with experimental animals for which obesity was induced through a 20 week high-fat diet. METHODS: High-fat feed was provided for 20-week-old SD rats in order to induce obesity for 20 weeks. The experimental animals were divided into a Normal Diet Control Group (ND-CON, n=6), a High-Fat Diet Control Group (HFD-CON, n=6) and a High-Fat Diet Exercise Group (HFD-EXE, n=6). The treadmill exercise was conducted, 30 minutes a day, four days a week for eight weeks. After the treadmill exercise, their weight, AUC and HOMA-IR were analyzed, and using the Western blot method, ER stress-related variables (GRP78/BiP, PERK, ATF6, IRE1α, eIF2α, XBP-1S & CHOP) were analyzed. RESULTS: In the HFD-CON Group, as compared to the ND-CON Group, the weight increased; blood glucose disposal capacity decreased; and HOMA-IR increased. In addition, the expression of ER stress-related variables, also, increased, so the signal transduction mechanism for cell death was activated. However, in the HFD-EXE Group that conducted treadmill exercise, as compared to the HFD-CON Group, the weight decreased; blood glucose disposal capacity improved; and HOMA-IR, also, decreased. ER stress-related variables decreased in the HFD-EXE Group as compared to the HFD-CON Group, and consequently, ER stress decreased. CONCLUSIONS: In conclusion, it was found that aerobic exercise like treadmill exercise would play positive roles in improving the imbalance of energy metabolism in the body induced by obesity and controlling ER stress induced from hippocampal tissue.

참고문헌 (42)

  1. Bertolotti A, "Dynamic interaction of BiP and ER stress transducers in the unfolded-protein response", Nature cell biology, 2(6), pp.326~332 (2000)
  2. Castro G, "Diet-induced obesity induces endoplasmic reticulum stress and insulin resistance in the amygdala of rats", FEBS open bio, 3, pp.443~449 (2013)
  3. Chapados NA, "Exercise training increases hepatic endoplasmic reticulum (er) stress protein expression in MTP-inhibited high-fat fed rats", Cell biochemistry and function, 28(3), pp.202~210 (2010)
  4. 최한율, "Ameliorative effect of myricetin on insulin resistance in mice fed a high-fat, high-sucrose diet", Nutrition Research and Practice, 8(5), pp.544~549 (2014), 한국영양학회
  5. da Luz G, "Endurance exercise training ameliorates insulin resistance and reticulum stress in adipose and hepatic tissue in obese rats", European journal of applied physiology, 111(9), pp.2015~2023 (2011)
  6. Delghingaro-Augusto V, "Voluntary running exercise prevents beta-cell failure in susceptible islets of the Zucker diabetic fatty rat", American journal of physiology Endocrinology and metabolism, 302(2), pp.254~264 (2012)
  7. Ding Y, "Grape seed proanthocyanidins ameliorate pancreatic beta-cell dysfunction and death in low-dose streptozotocin- and high-carbohydrate", high-fat diet-induced diabetic rats partially by regulating endoplasmic reticulum stress, 2013, pp.10, 51~
  8. Ding Y, "Intense exercise can cause excessive apoptosis and synapse plasticity damage in rat hippocampus through Ca2+ overload and endoplasmic reticulum stress-induced apoptosis pathway", Chinese medical journal, 127(18), pp.3265~3271 (2014)
  9. Drager LF, "Obstructive sleep apnea: a cardiometabolic risk in obesity and the metabolic syndrome", Journal of the American College of Cardiology, 62(7), pp.569~576 (2013)
  10. Ghosh AK, "Elevated Endoplasmic Reticulum Stress Response Contributes to Adipose Tissue Inflammation in Aging", The journals of gerontology Series A, Biological sciences and medical sciences (2014)[DOI]
  11. Hancock CR, "High-fat diets cause insulin resistance despite an increase in muscle mitochondria", Proceedings of the National Academy of Sciences of the United States of America, 105(22), pp.7815~7820 (2008)
  12. Harding HP, "Perk is essential for translational regulation and cell survival during the unfolded protein response", Molecular cell, 5(5), pp.897~904 (2000)
  13. Jiao P, "FFA-induced adipocyte inflammation and insulin resistance: involvement of ER stress and IKKbeta pathways", Obesity, 19(3), pp.483~491 (2011)
  14. Kang EB, "Treadmill exercise represses neuronal cell death and inflammation during Aβ-induced ER stress by regulating unfolded protein response in aged presenilin 2 mutant mice", Apoptosis, 18(11), pp.1332~1347 (2013)
  15. Kaufman RJ, "Stress signaling from the lumen of the endoplasmic reticulum: coordination of gene transcriptional and translational controls", Genes & development, 13(10), pp.1211~1233 (1999)
  16. Kawasaki N, "Obesity-induced endoplasmic reticulum stress causes chronic inflammation in adipose tissue", Scientific reports, 2, pp.799~ (2012)
  17. Kim HB, "Treadmill exercise increases cell proliferation in dentate gyrus of rats with streptozotocin-induced diabetes", Journal of diabetes and its complications, 17(1), pp.29~33 (2003)
  18. Lee J, "Unfolded protein response signaling and metabolic diseases", The Journal of biological chemistry, 289(3), pp.1203~1211 (2014)
  19. Lin JH, "Endoplasmic reticulum stress in disease pathogenesis", Annual review of pathology, 3, pp.399~425 (2008)
  20. Maris M, "Deletion of C", EBP homologous protein (Chop) in C57Bl, Diabetologia, pp.2012, 55(4)[DOI]
  21. Marques CM, "Beneficial effects of exercise training (treadmill) on insulin resistance and nonalcoholic fatty liver disease in high-fat fed C57BL", 6 mice, 2010, pp.43(5), 467~475
  22. McKenzie MJ, "Aerobic exercise bout effects on gene transcription in the rat soleus", Medicine and science in sports and exercise, 39(9), pp.1515~1521 (2007)
  23. McTiernan A, "Exercise effect on weight and body fat in men and women", Obesity, 15(6), pp.1496~1512 (2007)
  24. Mori K, "Tripartite management of unfolded proteins in the endoplasmic reticulum", Cell, 101(5), pp.451~454 (2000)
  25. O'Leary VB, "Exercise-induced reversal of insulin resistance in obese elderly is associated with reduced visceral fat", Journal of applied physiology, 100(5), pp.1584~1589 (2006)
  26. Ozcan L, "Endoplasmic reticulum stress plays a central role in development of leptin resistance", Cell metabolism, 9(1), pp.35~51 (2009)
  27. Ozcan U, "Endoplasmic reticulum stress links obesity, insulin action, and type 2 diabetes", Science, 306(5695), pp.457~461 (2004)
  28. Rao X, "Exercise protects against diet-induced insulin resistance through downregulation of protein kinase Cbeta in mice", PloS one, 8(12), pp.e81364~ (2013)
  29. Rector RS, "Exercise and diet induced weight loss improves measures of oxidative stress and insulin sensitivity in adults with characteristics of the metabolic syndrome", American journal of physiology Endocrinology and metabolism, 293(2), pp.500~506 (2007)
  30. Riccardi G, "Dietary fat, insulin sensitivity and the metabolic syndrome", Clinical nutrition, 23(4), pp.447~456 (2004)
  31. Ryu D, "Endoplasmic reticulum stress promotes LIPIN2-dependent hepatic insulin resistance", Diabetes, 60(4), pp.1072~1081 (2011)
  32. Salvado L, "PPARβ", δ prevents endoplasmic reticulum stressassociated inflammation and insulin resistance in skeletal muscle cells through an AMPK-dependent mechanism, 2014, pp.57(10), 2126~2135
  33. Schroder M, "The mammalian unfolded protein response", Annual review of biochemistry, 74, pp.739~789 (2005)
  34. Travers KJ, "Functional and genomic analyses reveal an essential coordination between the unfolded protein response and ER-associated degradation", Cell, 101(3), pp.249~258 (2000)
  35. Tucsek Z, "Obesity in aging exacerbates blood-brain barrier disruption, neuroinflammation, and oxidative stress in the mouse hippocampus: effects on expression of genes involved in Beta-amyloid generation and Alzheimer's disease", The journals of gerontology Series A, Biological sciences and medical sciences, 69(10), pp.1212~1226 (2014)
  36. Verkhratsky A, "Endoplasmic reticulum Ca(2+) homeostasis and neuronal death", Journal of cellular and molecular medicine, 7(4), pp.351~361 (2003)
  37. Whitmer RA, "The epidemiology of adiposity and dementia", Current Alzheimer research, 4(2), pp.117~122 (2007)
  38. Wu J, "The unfolded protein response mediates adaptation to exercise in skeletal muscle through a PGC-1alpha", ATF6alpha complex, 2011, pp.13(2), 160~169
  39. Yang J, "Protective effect of liraglutide against ER stress in the liver of high-fat diet-induced insulin-resistant rats", Endocrine (2014)[DOI]
  40. Yoshida H, "Identification of the cis-acting endoplasmic reticulum stress response element responsible for transcriptional induction of mammalian glucose-regulated proteins. Involvement of basic leucine zipper transcription factors", The Journal of biological chemistry, 273(50), pp.33741~33749 (1998)
  41. Yoshida H, "A time-dependent phase shift in the mammalian unfolded protein response", Developmental cell, 4(2), pp.265~271 (2003)
  42. Zhang X, "Hypothalamic IKKbeta", NF-kappaB and ER stress link overnutrition to energy imbalance and obesity, 2008, pp.135(1), 61~73

관련 논문AI추천

트레드밀 운동이 고지방식이를 섭취한 비만 쥐 골격근의소포체 스트레스에 미치는 영향강은범, 구정훈, 권만근, 권인수, 엄현섭, 오상훈, 이창국, 장용철, 조인호, 조준용, 홍석민국민체육진흥공단 한국스포츠과학원 · 체육과학연구2014 · 제25권 제3호트레드밀 운동이 고지방식으로 유도된 알츠하이머 치매 마우스에서 인슐린 수용체, 산화 스트레스 및 시냅스 가소성 장애에 미치는 효과김동현한국운동생리학회 · 운동과학2018 · 제27권 제3호트레드밀 운동이 고지방 식이 유도 비만 쥐의 골격근 인슐린 신호 경로에 미치는 영향강성훈, 김혜영, 신기옥, 여남회, 우진희국민체육진흥공단 한국스포츠과학원 · 체육과학연구2012 · 제23권 제3호12주간의 지구성 운동이 비만 흰쥐 해마의 도파민 수용체 단백질 발현에 미치는 영향김기진, 김성욱한국운동생리학회 · 운동과학2020 · 제29권 제1호트레드밀 운동이 ICV-STZ 처치 흰쥐의 뇌 인슐린 신호전달, 포도당 대사 및 Tau 단백질 과인산화에 미치는 영향강은범, 김창환, 조준용한국운동생리학회 · 운동과학2014 · 제23권 제2호중강도 운동이 고지방식을 유도한 알츠하이머 치매 마우스의 시냅스 소포체 및 항산화 효소 발현에 미치는 영향김동현, 김인재한국운동생리학회 · 운동과학2020 · 제29권 제2호

이 간행물의 다른 논문 (11건)

성인을 위한 신체활동 가이드라인 및 운동 강도에 대한 고찰김광준, 김창선, 박동호pp. 99-107CrossFit-based Training과 Weight Training이 중년남성의 건강 및 기능관련체력과 혈중지질에 미치는 영향유동훈pp. 109-116운동유발성 고혈압 마라톤주자의 심전도상 좌심실 비대와 QT분산에 관한 연구박순철, 김영주, 오재근pp. 117-123유산소운동 수준에 따른 중추신경계의 활동변화김승열pp. 125-132고강도 운동 시 식이형태가 근육 부상에 미치는 영향이규락, 강호율, 정현령pp. 133-141
원문 보기