트레드밀 운동이 고지방식이를 섭취한 비만 쥐 골격근의소포체 스트레스에 미치는 영향
Treadmill Exercise Reduces Endoplasmic Reticulum Stress in High-Fat Diet-Induced Obese Rats Partially by Regulating Unfolded Protein Response
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본 연구는 고지방식이로 유도된 비만 흰쥐를 대상으로 지구성 운동이 체중변화, 글루코스 내성 및 소포체 스트레스(ER-stress)에 반응에 관여하는 단백질 발현에 미치는 영향을 규명하는데 목적이 있다. 실험동물은 16주간 고지방식이를 먹인 흰쥐를 사용하였으며 집단은 Sprague-Dawley Control(SD-Con/n=4)집단, High Fat Control(HF-Con/n=4)집단, High Fat exercise(HF-Exe/n=4)으로 구분하고 트레드밀 운동을 하루 30분, 주 5회 총 8주간 21m/min의 속도로 실시하였다. 먼저, 체중과 글루코스 내성은 HF-Con 집단에서 유의하게 가장 높았지만 트레드밀 운동을 실시한 HF-Exe집단에서 유의한 감소를 나타났다. 또한 소포체 스트레스에 반응에 관여하는 glucose-regulated protein 78 (BiP/GRP78), activating transcription factor 6 (ATF6), Pancreatic ER kinase(PERK) 및 inositol-requiring 1α (IRE-1α)/X-box binding protein 1 (XBP-1)은 HF-Con 집단에서 유의하게 증가를 나타냈고, HF-Exe 집단에서 유의한 감소가 나타났다. 따라서 지구성 운동은 고지방 식이로 유도된 비만 휜쥐의 체중 감소와 글루코스 내성을 개선시키고, 소포체 스트레스를 감소시키는데 긍정적인 영향을 미치는 것으로 생각된다
Abstract
This study was to performed to the effect of 8-week endurance exercise influences on body weight, glucose tolerance and ER-stress in soleus of 16weeks Rats fed High-Fat diet. Rats were randomly assigned to 3 group; (1)Sprague-Dawley Control diet (SD-Con/n=4), (2)High-Fat diet Control (HF-Con/n=4), (3)High-Fat diet Exercise (HF-Exe/n=4). Exercise group ran on the treadmill for 30min/day at the level of 21m/min for 5days/week during 8weeks. Results showed that body weight and glucose tolerance of the HF-Con group was remarkably increased(p<.05) compared to other groups. However, HF-Exe group significantly decreased body weight and glucose tolerance compared to HF-Con group. Moreover, level of GRP78, ATF6, PERK and IER1⍺, which are main proteins of ER-stress were significantly increased in HF-Con group higher than other group, whereas HF-Exe group significantly decreased the expression of GRP78, ATF6, PERK and IER1⍺. Taken together, these finding suggested that the reduction of the body weight, glucose tolerance and unfolded protein response by treadmill exercise may represent a positive adaptation protecting against high-fat diet-induced ER stress.
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