일회성 운동이 골감소증 고령여성의 골대사성 사이토카인 생성에미치는 영향
The Effects of a Bout Exercise on Production of Bone Metabolic Cytokines in Osteopenia Elederly Women
1
조회
0
다운로드
4
인용
0
참고문헌
키워드
초록
PURPOSE: Although overproduction of cytokines from peripheral blood mononuclear cell (PBMC) was associated with the reduction of bone mass, bone metabolic cytokines production according to exercise is still not clear. We aimed to test whether changes in production of bone metabolic cytokines after a bout exercise in osteopenia elderly women. METHOD: Nine osteopenia elderly women (T-score -2.61±0.46; aged 67.67±3.24 years) underwent a bout exercise of 60 %VO2max (EX group) with a cycle ergometer. To clarify the changes during exercise, we designed repeated measure ANOVA as the sedentary control (CON group) to perform blood sampling without exercise. The concentrations and the mRNA expressions of cytokines were measured before, immediately after and then recovery 60 minutes after exercise. Serum Ca, Pi, Mg, and ALP were also measured at same times. RESULTS: There were no significant changes in the concentrations of serum TNF-alpha, IL-6, IL-1beta, and INF-gamma (NS). Although the mRNA expression of most cytokines also show no significant changes (NS), only TNF-alpha mRNA expression showed significant difference between groups (p=.008). On the other hand, serum Ca showed significant difference between times (p=.002), that of EX group was significantly increased at immediately after exercise compared to baseline (p<.05), and it was decreased at recovery period (p<.01). CONCLUSIONS: These results suggest that a bout exercise of moderate intensity might not be effect on production of bone metabolic cytokines both level of serum and mRNA from PBMC, and induced temporal hypercalcemia.
Abstract
PURPOSE: Although overproduction of cytokines from peripheral blood mononuclear cell (PBMC) was associated with the reduction of bone mass, bone metabolic cytokines production according to exercise is still not clear. We aimed to test whether changes in production of bone metabolic cytokines after a bout exercise in osteopenia elderly women. METHOD: Nine osteopenia elderly women (T-score -2.61±0.46; aged 67.67±3.24 years) underwent a bout exercise of 60 %VO2max (EX group) with a cycle ergometer. To clarify the changes during exercise, we designed repeated measure ANOVA as the sedentary control (CON group) to perform blood sampling without exercise. The concentrations and the mRNA expressions of cytokines were measured before, immediately after and then recovery 60 minutes after exercise. Serum Ca, Pi, Mg, and ALP were also measured at same times. RESULTS: There were no significant changes in the concentrations of serum TNF-alpha, IL-6, IL-1beta, and INF-gamma (NS). Although the mRNA expression of most cytokines also show no significant changes (NS), only TNF-alpha mRNA expression showed significant difference between groups (p=.008). On the other hand, serum Ca showed significant difference between times (p=.002), that of EX group was significantly increased at immediately after exercise compared to baseline (p<.05), and it was decreased at recovery period (p<.01). CONCLUSIONS: These results suggest that a bout exercise of moderate intensity might not be effect on production of bone metabolic cytokines both level of serum and mRNA from PBMC, and induced temporal hypercalcemia.
참고문헌 (35)
- Lewiecki EM, "Clinical applications of bone density testing for osteoporosis", Minerva Medica, 96(5), pp.317~330 (2005)
- Zmuda JM, "Genetic epidemiology of osteoporosis:past, present, and future", Current Osteoporosis Reports, 3(3), pp.111~115 (2005)
- Mundy GR, "Nutritional modulators of bone remodeling during aging", The American Journal of Clinical Nutrition, 83(2), pp.427S~430S (2006)
- Ferrari SL, "Osteoporosis: a complex disorder of aging with multiple genetic and environmental determinants", World Review of Nutrition and Dietetics, 95, pp.35~51 (2005)
- Ohshima H, "Bone loss and bone metabolism in astronauts during long-duration space flight", Clinical Calcium, 16(1), pp.81~85 (2006)
- Iwamoto J, "Interventions to prevent bone loss in astronauts during space flight", The Keio Journal of Medicine, 54(2), pp.55~59 (2005)
- Bonaiuti D, "Exercise for preventing and treating osteoporosis in postmenopausal women", The Cochrane Database of Systematic Reviews, (3), pp.CD000333~ (2002)
- Nuti R, "Professional sport activity and micronutrients: effects on bone mass", Journal of Endocrinological Investigation, 28(10), pp.52~60 (2002)
- Yamamoto T, "Mechanical stress induces expression of cytokines in human periodontal ligament cells", Oral Diseases, 12(2), pp.171~175 (2006)
- Pacifici R, "Bone matrix constituents stimulate interleukin-1 release from human blood mononuclear cells", Journal of Clinical Investigation, 87(1), pp.221~228 (1991)
- Bernard-Poenaru O, "Bone-resorbing cytokines from peripheral blood mononuclear cells after hormone replacement therapy: a longitudinal study", Osteoporosis International, 12(9), pp.769~776 (2001)
- Wang LC, "Osteoclastogenic activity during mandibular distraction osteogenesis", Journal of Dental Research, 84(11), pp.1010~1015 (2005)
- Xiong Y, "Identification of two groups of smoldering multiple myeloma patients who are either high or low producers of interleukin-1", Journal of Interferon & Cytokine Research, 26(2), pp.83~95 (2006)
- Giannini S, "Bone disease in primary hypercalciuria", Critical Reviews in Clinical Laboratory Sciences, 42(3), pp.229~248 (2005)
- Inanir A, "The effects of calcitriol therapy on serum interleukin-1, interleukin-6 and tumour necrosis factor-alpha concentrations in post-menopausal patients with osteoporosis", Journal of International Medical Research, 32(6), pp.570~582 (2004)
- De Maeyer-Guignard J, "Identification by in situ hybridization of IFN-beta-producing murine macrophages obtained from high and low interferon producers", Annales de l’Institut Pasteur, 1988, pp.139(1), 51~57
- Kotake S, "IFN-gamma-producing human T cells directly induce osteoclastogenesis from human monocytes via the expression of RANKL", European Journal of Immunology, 35(11), pp.3353~3363 (2005)
- Cheng J, "Molecular mechanisms of the biphasic effects of interferon-γ on osteoclastogenesis", Journal of Interferon & Cytokine Research, 32(1), pp.34~45 (2012)
- Kanis JA, "The diagnosis of osteoporosis", Journal of Bone and Mineral Research, 9(8), pp.1137~1141 (1994)
- Miyashita M, "Effects of physical training", Medicine and Science in Sports and Exercise, 1, pp.3~5 (1985)
- Chomczynski P, "Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction", Analytical Biochemistry, 162(1), pp.156~159
- Nowak A, "Biochemical markers of bone metabolism in healthy elderly men. The relationship to physical activity", The Aging Male, 8(2), pp.75~80 (2005)
- Suda T, "Modulation of osteoclast differentiation", Endocrine Reviews, 13(1), pp.66~80 (1992)
- Ishimi Y, "IL-6 is produced by osteoblasts and induces bone resorption", The Journal of Immunology, 145(10), pp.3297~3303 (1990)
- Kim CS, "Compulsive running exercise induce osteopenia in stroke-prone spontaneously hypertensive rats", Journal of the human-environment system, 2(1), pp.47~56 (1999)
- Kim CS, "Effects of chronic NH4Cl dosage and swimming exercise on bone metabolic turnover in rats", Journal of Physiological Anthropology and Applied Human Science, 24(6), pp.595~600 (2005)
- DeRijk RH, "Induction of plasma interleukin-6 by circulating adrenaline in the rat", Psychoneuroendocrinology, 19(2), pp.155~163 (1994)
- Ostrowski K, "A trauma-like elevation of plasma cytokines in humans in response to treadmill running", The Journal of Physiology, 513(Pt3), pp.889~894 (1998)
- Kim CS, "The effects of all-out anaerobic exercise on bone metabolic turnover", 2007 international sport science congress, pp.196~ (2007), KAHPERD
- 김창선, "Expression of bone-resorbing cytokines according to aerobic exercise intensities", Journal of Exercise Nutrition & Biochemistry, 15(3), pp.131~139 (2011), 한국운동영양학회
- 박동호, "성인을 위한 신체활동 가이드라인 및 운동 강도에 대한 고찰", 운동과학, 24(2), pp.99~107 (2015), 한국운동생리학회[DOI]
- Marques EA, "Response of bone mineral density, inflammatory cytokines, and biochemical bone markers to a 32-week combined loading exercise programme in older men and women", Archives of Gerontology and Geriatrics, 57(2), pp.226~233 (2013)
- Golzari Z, "Combined exercise training reduces IFN-gamma and IL-17 levels in the plasma and the supernatant of peripheral blood mononuclear cells in women with multiple sclerosis", International Immunopharmacology, 10(11), pp.1415~1419 (2010)
- Touvra AM, "Combined strength and aerobic training increases transforming growth factor beta 1 in patients with type 2 diabetes", Hormones (Athens), 10(2), pp.125~130 (2011)
- Mundy GR, "The hypercalcemia of malignancy", Kidney International, 31(1), pp.142~155 (1987)