ICR mouse의 피부와 간에서 자외선 B파에 의한 Heme Oxygenase-1의 유도
Heme Oxygenase-1 (HO-1) Induction by UVB (290-320nm) Radiation in ICR Mice
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The induction of heme oxygenase-1(HO-1) by UV radiation provides a protective defense against oxidative stress, and has been well demonstrated in UVA-irradiated skin, but not UVB. In this study, we show that the UVB radiation can be attributed to the induction of cutaneous HO-1. The expression of HO-1 mRNA was assessed in vivo by reverse transcription-polymerase chain reaction(RT-PCR) analysis, and HO enzyme activity was measured in microsomal preparation from irradiated mice. The mRNA level of HO-1 increases in liver and skin from 1d to 3d after UVB(3KJ/m²) exposure. The results of gene expression were same pattern of HO enzyme activity in skin, but not in liver. HO-1 mRNA in liver resulted in a progressive increase to 4d after UVB exposure, but HO activity in liver increased to 2d. This finding indicates that UVB radiation is an important inducer of HO-1 and increases in HO activity may protect tissue directly or indirectly from oxidative stress.
Abstract
The induction of heme oxygenase-1(HO-1) by UV radiation provides a protective defense against oxidative stress, and has been well demonstrated in UVA-irradiated skin, but not UVB. In this study, we show that the UVB radiation can be attributed to the induction of cutaneous HO-1. The expression of HO-1 mRNA was assessed in vivo by reverse transcription-polymerase chain reaction(RT-PCR) analysis, and HO enzyme activity was measured in microsomal preparation from irradiated mice. The mRNA level of HO-1 increases in liver and skin from 1d to 3d after UVB(3KJ/m²) exposure. The results of gene expression were same pattern of HO enzyme activity in skin, but not in liver. HO-1 mRNA in liver resulted in a progressive increase to 4d after UVB exposure, but HO activity in liver increased to 2d. This finding indicates that UVB radiation is an important inducer of HO-1 and increases in HO activity may protect tissue directly or indirectly from oxidative stress.
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