Hairless 마우스에서 LED 광 조사의 피부주름 개선효과
Inhibitory Effect of LED Light Irradiation on the Wrinkle Formation in Hairless Mouse
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초록
To induce the skin aging in hairless mouse, UVB irradiation and squalene monohydroperoxide (Sq-OOH) were applied to the back region. Then, light emitting diode (LED) irradiation was applied to the same region 6 days a week for 4 weeks 2(wavelength: 635 nm, quantity of light=3.5 mW/cm , group E1: 30 minutes a day, group E2: 60 minutes a day). According to gross observation of wrinkle formation on skin tissues, experimental groups (E1 and E2) showed thinner ridge line, narrower ridge spacing, and shallower wrinkle formation than control (C group). In quantitative analysis, E1 and E2 groups showed a significant improvement in wrinkle parameters than C group. Positive control (PC group), E1, and E2 groups showed higher density of intradermal collagen fiber and higher regularity of its arrangement than C group. E1 and E2 groups showed lower degenerated elastic fiber than C group. On the other hand, PC, E1, and E2 groups showed significantly lower dermal xanthine oxidase (XO) activity than C group. And E1 and E2 groups showed higher dermal superoxide dismutase (SOD) activity than C and PC groups. In comparison with N group, E1 and E2 groups showed similar level of dermal catalase (CAT) activity and matrix metalloproteinase-3 (MMP-3) expression. Summing up, it is concluded that LED irradiation is effective in control or improving skin wrinkle formation by inhibiting generation of reactive oxygen species (ROS) and facilitating elimination of generated ROS. And E2 group is more effective than E1 group, which indicates that the effects of LED irradiation depend on irradiation time.
Abstract
To induce the skin aging in hairless mouse, UVB irradiation and squalene monohydroperoxide (Sq-OOH) were applied to the back region. Then, light emitting diode (LED) irradiation was applied to the same region 6 days a week for 4 weeks 2(wavelength: 635 nm, quantity of light=3.5 mW/cm , group E1: 30 minutes a day, group E2: 60 minutes a day). According to gross observation of wrinkle formation on skin tissues, experimental groups (E1 and E2) showed thinner ridge line, narrower ridge spacing, and shallower wrinkle formation than control (C group). In quantitative analysis, E1 and E2 groups showed a significant improvement in wrinkle parameters than C group. Positive control (PC group), E1, and E2 groups showed higher density of intradermal collagen fiber and higher regularity of its arrangement than C group. E1 and E2 groups showed lower degenerated elastic fiber than C group. On the other hand, PC, E1, and E2 groups showed significantly lower dermal xanthine oxidase (XO) activity than C group. And E1 and E2 groups showed higher dermal superoxide dismutase (SOD) activity than C and PC groups. In comparison with N group, E1 and E2 groups showed similar level of dermal catalase (CAT) activity and matrix metalloproteinase-3 (MMP-3) expression. Summing up, it is concluded that LED irradiation is effective in control or improving skin wrinkle formation by inhibiting generation of reactive oxygen species (ROS) and facilitating elimination of generated ROS. And E2 group is more effective than E1 group, which indicates that the effects of LED irradiation depend on irradiation time.
참고문헌 (29)
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