본문 바로가기
KERD
논문KCI 등재

장기간 운동이 마우스 조직별 heme oxygenase-1 발현에 미치는 영향

Effects of long-term treadmill exercise on heme oxygenase-1 expression in mouse tissues

김시영
한국운동생리학회|2010.02|운동과학 (Exercise Science)|제19권 제1호|pp. 15-26|DOI 10.15857/ksep.2010.19.1.15

0

조회

0

다운로드

5

인용

0

참고문헌

키워드

Heme oxygenase-1장기간 운동마우스 조직Long-term ExerciseMouse Tissues

초록

Heme oxygenase-1 (HO-1)은 산화적인 자극을 포함한 다양한 외부 스트레스에 대한 조직세포의 적응 및 방어에 중요한 역할을 담당하는 항산화효소이다. 본 연구의 목적은 마우스의 다양한 조직에서 HO-1의 발현 차이는 물론 장기간의 운동에 의한 조절 가능성을 알아보는데 있다. 연구에 사용된 24마리의 ICR계 마우스는 각각 8마리씩 세 그룹 [control group (CONT, n=8), low-intensity exercise group (LIE, n=8), high-intensity exercise group (HIE, n=8)]으로 나누어 졌으며, 16주간의 트레드밀 운동프로그램 후 골격근, 대장, 심장, 폐, 간 그리고 뇌를 분리하여 분석하였다. CONT에서 안정시 마우스 조직별 HO-1 발현량을 분석한 결과, 간 조직에서의 발현량이 가장 높았으며, 이어서 대장, 폐, 심장, 골격근 그리고 뇌 순으로 발현량의 유의한 차이가 나타났다. 16주간 트레이닝 후 비교그룹 (CONT)에 비해 트레이닝 그룹 (HIE, LIE)의 골격근, 대장 및 간에서 HO-1의 발현량이 유의하게 증가된 (p<.01, p<.001) 반면, 심장, 폐 그리고 뇌 조직에서는 그룹 간 발현차이가 나타나지 않았다. 이상의 결과를 바탕으로 결론을 내리면, 안정된 상태에서 마우스 조직에 따라 발현되는 HO-1 단백질량은 조직 사이에 유의한 차이가 있으며, 골격근, 대장 그리고 간 조직에서의 HO-1 발현량은 반복된 운동으로 증가시킬 수 있었다.

Abstract

Heme oxygenase (HO)-1, involved in the heme degradation process, is upregulated by a wide array of stimuli and has antioxidant, anti-inflammatory and other cytoprotective functions. The aim of this study was to investigate the difference and effect of long-term exercise on HO-1 expression among mouse tissues. The study carried out on twenty-four ICR mice. All mice participated in this study which consisted of three groups [control group (CONT, n=8), low-intensity exercise group (LIE, n=8), high-intensity exercise group (HIE, n=8)]. Mice were euthanized after 16 weeks of training periods and tissues (skeletal muscle, colon, heart, lung, liver, and brain) were removed. As a result of analyzed difference of HO-1 expression among tissues, liver topped the list, followed by colon, lung, heart, skeletal muscle, and brain (p<.001). In case of skeletal muscle, colon, and liver, after training for 16 weeks, HO-1 expression in HIE and/or LIE dramatically increased in comparison with CONT (p<.01, p<.001). On the other hand, no significant differences in the heart, lung, and brain were found between all groups. Taken together, we conclude that level of HO-1 expression is different between mouse tissues and long-term regular exercise can enhance the levels of HO-1 expression in skeletal muscle, colon, and liver.

참고문헌 (74)

  1. 김종오, "운동강도가 F344 흰쥐 심장과 폐 조직의 항산화 효소 활성도 및 조직 형태학적 발육 변화에 미치는 영향", 한국발육발달학회지, 11(2), pp.22~37 (2003), 한국발육발달학회
  2. 이복환, "운동강도에 따른 지질 과산화물과 체내 항산화효소의 활성정도와 항산화제의 방어효과", 한국체육학회지, 40(3), pp.661~674 (2001)
  3. 통계청, "암등록통계: 10대 암 발생률 및 5년 상대 생존율 (2002-2005)" (2009), 통계청
  4. Abraham, N. G, "Heme oxygenase-1 attenuates glucose-mediated cell growth arrest and apoptosis in human microvessel endothelial cells", Circ. Res.,, 93(6), pp.507~514 (2003)
  5. Alcaraz, M. J, "Anti-inflammatory actions of the heme oxygenase-1 pathway", Curr. Pharm. Des, 9(30), pp.2541~2551 (2003)
  6. Alessio, H. M, "Lipid peroxidation and scavenger enzymes during exercise: adaptive response to training", J. Appl. Physiol, 64(4), pp.1333~1336 (1988)
  7. Attuwaybi, B. O, "Heme oxygenase-1 induction by hemin protects against gut ischemia", reperfusion injury, 2004, pp.118(1), 53~57
  8. Balkwill, F, "Inflammation and cancer: back to Virchow?", Lancet, 357(9255), pp.539~545 (2001)
  9. Barel, M, "Exercise training prevents hyperinsulinemia, muscular glycogen loss and muscle atrophy induced by dexamethasone treatment" (2009), Eur. J. Appl. Physiol. in press.
  10. Berk, L. S, "The effect of long endurance running on natural killer cells in marathoners", Med. Sci. Sports. Exerc, 22(2), pp.207~212 (1990)
  11. Boyum, A, "The effect of strenuous exercise, calorie deficiency and sleep deprivation on white blood cells, plasma immunoglobulins and cytokines", Scand. J. Immunol, 43(2), pp.228~235 (1996)
  12. Calton, B. A, "Physical activity and the risk of colon cancer among women: a prospective cohort study (United States)", Int. J. Cancer, 119(2), pp.385~391 (2006)
  13. Cannon, J. G, "Exercise enhances survival rate in mice infected with Salmonella typhimurium", Proc. Soc. Exp. Biol. Med, 175(4), pp.518~521 (1984)
  14. Chao, A, "Amount, type, and timing of recreational physical activity in relation to colon and rectal cancer in older adults: the Cancer Prevention Study II Nutrition Cohort. Cancer Epidemiol", Biomarkers Prev, 13(12), pp.2187~2195 (2004)
  15. Cho, H. Y, "Role of NRF2 in protection against hyperoxic lung injury in mice", Am. J. Respir. Cell Mol. Biol, 26(2), pp.175~182 (2002)
  16. Choi, B. M, "1,2,3,4,6-Penta-O-galloyl-beta-D- glucose protects rat neuronal cells (Neuro 2A) from hydrogen peroxide-mediated cell death via the induction of heme oxygenase-1", Neurosci. Lett, 328(2), pp.185~189 (2002)
  17. Clevers, H, "At the crossroads of inflammation and cancer", Cell, 118(6), pp.671~674 (2004)
  18. Colbert, L. H, "Negative energy balance induced by voluntary wheel running inhibits polyp development in APCMin mice", Carcinogenesis, 27(10), pp.2103~2107 (2006)
  19. Damle, S. S, "Hemoglobin-based oxygen carrier induces heme oxygenase-1 in the heart and lung but not brain", J. Am. Coll. Surg.,, 208(4), pp.592~598 (2009)
  20. Fang, J, "Antiapoptotic role of heme oxygenase (HO) and the potential of HO as a target in anticancer treatment", Apoptosis, 9(1), pp.27~35 (2004)
  21. Fang, J, "Enhancement of chemotherapeutic response of tumor cells by a heme oxygenase inhibitor, pegylated zinc protoporphyrin", Int. J. Cancer, 109(1), pp.1~8 (2004)
  22. Fujii, H, "Protective role of heme oxygenase-1 in the intestinal tissue injury in an experimental model of sepsis", Crit. Care Med, 31(3), pp.893~902 (2003)
  23. Gong, P, "Heme oxygenase-1 protects HepG2 cells against cytochrome P450 2E1-dependent toxicity", Free Radic. Biol. Med, 36(3), pp.307~318 (2004)
  24. Hasegawa, Y, "Decreased expression of glutathione peroxidase mRNA in thyroid anaplastic carcinoma", Cancer Lett, 182(1), pp.69~74 (2002)
  25. Hollander, J, "Superoxide dismutase gene expression in skeletal muscle: fiber-specific adaptation to endurance training", Am. J. Physiol, 277(3Pt2), pp.R856~R862 (1999)
  26. Huang, E, "Heme oxygenase-1 activity after excitotoxic injury: immunohistochemical localization of bilirubin in neurons and astrocytes and deleterious effects of heme oxygenase inhibition on neuronal survival after kainate treatment", J. Neurosci. Res, 80(2), pp.268~278 (2005)
  27. Jenkins, R. R, "Free radical chemistry. Relationship to exercise", Sports Med, 5(3), pp.156~170 (1988)
  28. Jensen, G. L, "Inflammation: roles in aging and sarcopenia", JPENJ Parenter Enteral Nutr, 32(60), pp.656~659 (2008)
  29. Ji, L. L, "Exercise and oxidative stress: role of the cellular antioxidant systems", Exerc. Sport. Sci. Rev, (23), pp.135~166 (1995)
  30. Ji, L. L, "Glutathione and antioxidant enzymes in skeletal muscle: effects of fiber type and exercise intensity", J. Appl. Physiol, 73(5), pp.1854~1859 (1992)
  31. Kamimoto, M, "Reciprocal regulation of IL-6 and IL-10 balance by HGF via recruitment of heme oxygenase-1 in macrophages for attenuation of liver injury in a mouse model of endotoxemia", Int. J. Mol. Med, 24(2), pp.161~170 (2009)
  32. Kumar, C. T, "Dietary supplementation of vitamin E protects heart tissue from exercise-induced oxidant stress", Mol. Cell Biochem, 111(1), pp.109~115 (1992)
  33. Langouet, S, "Inhibition of human cytochrome P450 enzymes by 1,2-dithiole-3-thione, oltipraz and its derivatives, and sulforaphane", Chem. Res. Toxicol, 13(4), pp.245~252 (2000)
  34. Laughlin, M. H, "Skeletal muscle oxidative capacity, antioxidant enzymes, and exercise training", J. Appl. Physiol, 68(6), pp.2337~2343 (1990)
  35. Leeuwenburgh, C, "Adaptations of glutathione antioxidant system to endurance training are tissue and muscle fiber specific", Am. J. Physiol, 272(1Pt2), pp.R363~R369 (1997)
  36. Lund Nilsen, T. I, "Colorectal cancer associated with BMI, physical activity, diabetes, and blood glucose", IARC Sci. Publ, (156), pp.257~258 (2002)
  37. Maines, M. D, "Corticosterone promotes increased heme oxygenase-2 protein and transcript expression in the newborn rat brain", Brain Res, 722(1), pp.83~94 (1996)
  38. Marini, M, "Mild exercise training, cardioprotection and stress genes profile", Eur. J. Appl. Physiol, 99(5), pp.503~510 (2007)
  39. Marini, M, "Role of adapted physical activity to prevent the adverse effects of the sarcopenia. A pilot study", Ital. J. Anat. Embryol, 113(4), pp.217~225 (2008)
  40. Marx, J, "Cancer research. Inflammation and cancer: the link grows stronger", Science, 306(5698), pp.966~968 (2004)
  41. McCoubrey, W. K, "Isolation and characterization of a cDNA from the rat brain that encodes hemoprotein heme oxygenase-3", Eur. J. Biochem, 247(2), pp.725~732 (1997)
  42. Morimoto, K, "Cytoprotective role of heme oxygenase (HO)-1 in human kidney with various renal diseases", Kidney Int, 60(5), pp.1858~1866 (2001)
  43. Munday, R, "Induction of phase II detoxification enzymes in rats by plant-derived isothiocyanates: comparison of allyl isothiocyanate with sulforaphane and related compounds", J. Agric. Food Chem, 52(7), pp.1867~1871 (2004)
  44. Nakao, A, "Biliverdin protects the functional integrity of a transplanted syngeneic small bowel", Gastroenterology, 127(2), pp.595~606 (2004)
  45. Nieman, D. C, "Physical activity and immune function in elderly women", Med. Sci. Sports Exerc, 25(7), pp.823~831 (1993)
  46. Nieman, D. C, "The immune response to exercise", Semin. Hematol, 31(2), pp.166~179 (1994)
  47. Otterbein, L, "Hemoglobin provides protection against lethal endotoxemia in rats: the role of heme oxygenase-1", Am. J. Respir. Cell Mol. Biol., 13(5), pp.595~601 (1995)
  48. Otterbein, L. E, "Heme oxygenase: colors of defense against cellular stress", Am. J. Physiol. Lung Cell Mol. Physiol, 279(6), pp.L1029~L1037 (2000)
  49. Perrella, M. A, "Role of heme oxygenase-1 in cardiovascular function", Curr. Pharm. Des, 9(30), pp.2479~2487 (2003)
  50. Peters, C, "Exercise, cancer and the immune response of monocytes", Anticancer Res, 15(1), pp.175~179 (1995)
  51. Pomeraniec, Y, "Adrenocorticotropin induces heme oxygenase-1 expression in adrenal cells", J. Endocrinol, 180(1), pp.113~124 (2004)
  52. Powers, E. L, "Comments on "Quantification of radiation-induced hydroxyl radicals within nucleohistones using a molecular fluorescent probe"", Radiat. Res, 141(2), pp.227~228 (1995)
  53. Quan, S, "Functional expression of human heme oxygenase-1 (HO-1) driven by HO-1 promoter in vitro and in vivo", J. Cell Biochem, 85(2), pp.410~421 (2002)
  54. Rensing, H, "Differential activation pattern of redox-sensitive transcription factors and stress-inducible dilator systems heme oxygenase-1 and inducible nitric oxide synthase in hemorrhagic and endotoxic shock", Crit. Care Med, 29(10), pp.1962~1971 (2001)
  55. Rittweger, J, "Recovery of muscle atrophy and bone loss from 90 days bed rest: results from a one-year follow-up", Bone, 44(2), pp.214~224 (2009)
  56. Ryter, S. W, "Heme oxygenase-1: redox regulation of a stress protein in lung and cell culture models", Antioxid. Redox Signal, 7(1), pp.80~91 (2005)
  57. Sato, K, "Negative regulation of catalase gene expression in hepatoma cells", Mol. Cell Biol, 12(6), pp.2525~2533 (1992)
  58. Schipper, H. M, "Heme oxygenase-1: role in brain aging and neurodegeneration", Exp. Gerontol, 35(6), pp.821~830 (2000)
  59. Segal, R. J, "Randomized controlled trial of resistance or aerobic exercise in men receiving radiation therapy for prostate cancer", J. Clin. Oncol, 27(3), pp.344~351 (2009)
  60. Shephard, R. J, "Effects of exercise and training on natural killer cell counts and cytolytic activity: a meta-analysis", Sports Med, 28(3), pp.177~195 (1999)
  61. Slattery, M. L, "Physical activity and colon cancer: confounding or interaction?", Med. Sci. Sports Exerc, 34(6), pp.913~919 (2002)
  62. Tamion, F, "Induction of heme-oxygenase-1 prevents the systemic responses to hemorrhagic shock", Am. J. Respir. Crit. Care Med, 164(10Pt1), pp.1933~1938 (2001)
  63. Tiidus, P. M, "Antioxidant and oxidative enzyme adaptations to vitamin E deprivation and training", Med. Sci. Sports Exerc, 26(3), pp.354~359 (1994)
  64. Uehara, K, "The lower intestinal tract-specific induction of heme oxygenase-1 by glutamine protects against endotoxemic intestinal injury", Crit. Care Med, 33(2), pp.381~390 (2005)
  65. Vicente, A. M, "Beneficial effects of heme oxygenase-1 up-regulation in the development of experimental inflammation induced by zymosan", J. Pharmacol. Exp. Ther, 307(3), pp.1030~1037 (2003)
  66. Vile, G. F, "Heme oxygenase 1 mediates an adaptive response to oxidative stress in human skin fibroblasts", Proc. Natl. Acad. Sci. USA, 91(7), pp.2607~2610 (1994)
  67. Wang, X. H, "Heme oxygenase-1 alleviates ischemia", reperfusion injury in aged liver, 2005, pp.11(5), 690~694
  68. Willis, D, "Expression and modulatory effects of heme oxygenase in acute inflammation in the rat", Inflamm. Res, 44(Suppl 2), pp.S218~S220 (1995)
  69. Willis, D, "Heme oxygenase: a novel target for the modulation of the inflammatory response", Nat. Med, 2(1), pp.87~90 (1996)
  70. Woods, J. A, "Exercise, monocyte", macrophage function, and cancer, 1994, pp.26(2), 147~156
  71. Xia, Z. Y, "Ischaemic post-conditioning protects lung from ischaemia-reperfusion injury by up-regulation of haeme oxygenase-1" (2009), Injury. in press.
  72. Yachie, A, "Heme oxygenase and its role in defense system; paradigm shift of anti-inflammatory therapy", Nihon Rinsho Meneki Gakkai Kaishi, 30(1), pp.11~21 (2007)
  73. Yamanaka, N, "Superoxide dismutase activity in WI-38 cell cultures: effects of age, trypsinization and SV-40 transformation", Physiol. Chem. Phys, 6(2), pp.95~106 (1974)
  74. Zhang, M, "Overexpression of heme oxygenase-1 protects smooth muscle cells against oxidative injury and inhibits cell proliferation", Cell Res, 12(2), pp.123~132 (2002)

이 간행물의 다른 논문 (8건)

여자 고령자의 인플루엔자 백신접종 후 레크리에이션 댄스 및 요가가 면역반응 및 체력에 미치는 영향김기진, 공경민pp. 1-14복부비만으로 인한 인슐린 저항성 유발에 관한 기전 연구김동현, 이신호, 강현식, 조진경pp. 27-36기초군사훈련에 따른 사관생도의 체력향상과 체력검정제도 개선방안신승환, 박재영, 우재홍, 전태원, 박익렬pp. 37-48노인들의 운동습관이 감각조절 및 운동조절기능에 미치는 영향옥정석, 박우영pp. 49-58규칙적인 달리기 참여자의 심혈관 기능 특징남상남, 김상엽, 안정훈pp. 59-66
원문 보기