나노물질의 세포독성 평가법으로 Colony Forming Efficiency Assay에 대한 검증연구
Pre-validation of Colony Forming Efficiency Assay for Assessing the Cytotoxicity of Nanomaterials
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Objectives: The cytotoxcities of Au, Ag, SWCNT, SiO2, and ZnO nanomaterials were evaluated in order toassess their potential toxicological effects in in vitro cell models using colony forming efficiency (CFE) assay. Methods: The CFE assay of the test materials was carried out on Hep G2 cells. The size distribution ofnanomaterials was studied by transmission electron microscopy (TEM). Changes in cell viability after treatmentwith a toxicant will result in a decreased number of colonies formed in comparison to solvent. Results: The TEM images show that all the particles except SWCNT and ZnO can be considered approximatelyspherical. The gold and SiO2 nanoparticles show no response (no toxicity) in concentration responseexperiments. A statistically significant toxic effect was found in Hep G2 cells treated with Ag, SWCNT and ZnOnanomaterials. Conclusion: In this study, we considered CFE assay to be a promising test for screening studies for cytotoxicitywith physicochemical analysis.
Abstract
Objectives: The cytotoxcities of Au, Ag, SWCNT, SiO2, and ZnO nanomaterials were evaluated in order toassess their potential toxicological effects in in vitro cell models using colony forming efficiency (CFE) assay. Methods: The CFE assay of the test materials was carried out on Hep G2 cells. The size distribution ofnanomaterials was studied by transmission electron microscopy (TEM). Changes in cell viability after treatmentwith a toxicant will result in a decreased number of colonies formed in comparison to solvent. Results: The TEM images show that all the particles except SWCNT and ZnO can be considered approximatelyspherical. The gold and SiO2 nanoparticles show no response (no toxicity) in concentration responseexperiments. A statistically significant toxic effect was found in Hep G2 cells treated with Ag, SWCNT and ZnOnanomaterials. Conclusion: In this study, we considered CFE assay to be a promising test for screening studies for cytotoxicitywith physicochemical analysis.
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