SP-ICP-MS 및 ICP-MS를 이용한 HepG2 세포 내금 나노물질 축적 평가
Assessment of Gold Nanomaterial Uptake in HepG2 Cells by Single Particle ICP-MS and ICP-MS
1
조회
0
다운로드
1
인용
0
참고문헌
키워드
초록
The cellular uptake of gold nanomaterials (AuNPs) was investigated in human liver HepG2 cells exposed to 0.5 or 1 µg/mL of AuNPs for 24 hours. After the exposure period, the total gold concentration in the medium and cells was observed to have increased significantly in a dose-dependent manner; gold nanomaterials were mainly present in the medium, while some were present in the cells. SP-ICP-MS showed that the AuNPs concentration increased in the medium and cells, but the results were lower in comparison to the total gold concentration measured by ICP-MS. This difference was presumably due to the fact that AuNPs were ionized and aggregated, and only a part of them retained a shape that is detectable by SP-ICP-MS. The median size of AuNPs remained similar after 24 hours to that at initial exposure. In conclusion, this study confirmed the applicability of SP-ICP-MS for the analysis of the concentration and size of AuNPs and the distribution pattern of AuNPs in HepG2 cells
Abstract
The cellular uptake of gold nanomaterials (AuNPs) was investigated in human liver HepG2 cells exposed to 0.5 or 1 µg/mL of AuNPs for 24 hours. After the exposure period, the total gold concentration in the medium and cells was observed to have increased significantly in a dose-dependent manner; gold nanomaterials were mainly present in the medium, while some were present in the cells. SP-ICP-MS showed that the AuNPs concentration increased in the medium and cells, but the results were lower in comparison to the total gold concentration measured by ICP-MS. This difference was presumably due to the fact that AuNPs were ionized and aggregated, and only a part of them retained a shape that is detectable by SP-ICP-MS. The median size of AuNPs remained similar after 24 hours to that at initial exposure. In conclusion, this study confirmed the applicability of SP-ICP-MS for the analysis of the concentration and size of AuNPs and the distribution pattern of AuNPs in HepG2 cells.
참고문헌 (23)
- 나노물질 유통량 조사(IV) 및 산업계 안전관리지원 시범사업, "환경부", 29~50 (2015)
- M. E. Vance, "Nanotechnology in the real world: Redeveloping the nanomaterial consumer products inventory", Beilstein Journal of Nanotechnology, 21, pp.1769~1780 (2015)
- K. Kettler, "Cellular uptake of nanoparticles as determined by particle properties, experimental conditions, and cell type", Environmental Toxicology and Chemistry, 33, pp.481~492 (2014)[DOI]
- J.K. Fard, "A review of molecular mechanisms involved in toxicity of nanoparticles", Advanced Pharmaceutical Bulletin, 5, pp.447~454 (2015)[DOI]
- Y. Wang, "Cytotoxicity, DNA damage, and apoptosis induced by titanium dioxide nanoparticles in human nonsmall cell lung cancer A549 cells", Environmental Science and Pollution Research, 22, pp.5519~5530 (2015)[DOI]
- Y. Xue, "Cytotoxicity and apoptosis induced by silver nanoparticles in human liver HepG2 cells in different dispersion media", Journal of Applied Toxicology, 36, pp.352~360 (2016)[DOI]
- M. Milic, "Cellular uptake and toxicity effects of silver nanoparticles in mammalian kidney cells", Journal of Applied Toxicology, 35, pp.581~592 (2015)[DOI]
- A. R. Gliga, "Size-dependent cytotoxicity of silver nanoparticles in human lung cells: the role of cellular uptake, agglomeration and Ag release", Particle and Fibre Toxicology (2014)
- H. J. Yen, "Cytotoxicity and immunological response of gold and silver nanoparticles of different sizes", Small, 5, pp.1553~1561 (2009)[DOI]
- D. Bao, "Characterization of silver nanoparticles internalized by Arabidopsis plants using single particle ICP-MS analysis", Frontiers in Plant Science (2016)
- J. Jimenez-Lamana, "Single particle ICP-MS characterization of platinum nanoparticles uptake and bioaccumulation by Lepidium sativum and Sinapis alba plants", Journal of Analytical Atomic Spectrometry, 31, pp.2321~2329 (2016)[DOI]
- K. Loeschner, "Use of alkaline or enzymatic sample pretreatment prior to characterization of gold nanoparticles in animal tissue by single-particle ICPMS", Analytical and Bioanalytical Chemistry, 406, pp.3845~3851 (2014)[DOI]
- K. Loeschner, "Detection and characterization of silver nanoparticles in chicken meat by asymmetric flow field flow fractionation with detection by conventional or single particle ICP-MS", Analytical and Bioanalytical Chemistry, 405, pp.8185~8195 (2013)[DOI]
- I. L. Hsiao, "Quantification and visualization of cellular uptake of <TEX>$TiO_2$<", TEX> and Ag nanoparticles: comparison of different ICP-MS techniques, 2016, pp.14, 1~13
- H. Klingberg, "Uptake of gold nanoparticles in primary human endothelial cells", Toxicology Research, 4, pp.655~666 (2015)[DOI]
- L. Yang, "Comparisons of the biodistribution and toxicological examinations after repeated intravenous administration of silver and gold nanoparticles in mice", Scientific Reports, 7, pp.3303~ (2017)[DOI]
- E. E. Connor, "Gold nanoparticles are taken up by human cells but do not cause acute cytotoxicity", Small, 1, pp.325~327 (2005)[DOI]
- A. Barreto, "Behavior or colloidal gold nanoparticles in different ionic strength media", Journal of Nanoparticle Research, 17, pp.1~13 (2015)[DOI]
- W. Cui, "Effect of aggregation and the surface properties of gold nanoparticles on cytotoxicity and cell growth", Nanomedicine: Nanotechnology, Biology, and Medicine, 8, pp.46~53 (2012)[DOI]
- I. Canton, "Endocytosis at the nanoscale", Chemical Society Reviews, 41, pp.2718~2739 (2012)[DOI]
- S. Sabella, "A general mechanism for intracellular toxicity of metal-containing nanoparticles", Nanoscale, 6, pp.7052~7061 (2014)[DOI]
- V. A. VanWinkle, "Nanoparticle (NP) uptake by type-alveolar epithelial cells and their oxidant stress response", Nanotoxicology, 3, pp.307~318 (2009)[DOI]
- C. Freese, "Uptake and cytotoxicity of citrate-coated gold nanospheres: Comparative studies on human endothelial and epithelial cells", Particle and Fibre Toxicology, 9, pp.23~ (2012)[DOI]