Rhodococcus sp. EH831에 의한 벤젠, 톨루엔 및 에틸벤젠의 호기성 생분해에 미치는 에탄올의 영향
Effect of Ethanol on Aerobic Biodegradation of Benzene, Toluene, and Ethylbenzene by Rhodococcus sp. EH831
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The usage of ethanol (EtOH)-blended gasoline (gasohol), has been increasing in recent years. EtOH has influence on the distribution and biodegradation of aromatic compounds such as BTEX (benzene (B), toluene (T), ethylbenzene (B), and xylene (X)) that are gasoline compositions. In this study, the effect of EtOH on the aerobic biodegradation of B, T and E was investigated using a BTEX and EtOH-degrading bacterium, Rhodococcus sp. EH831. The degradation rates of B in the conditions of 1:1, 1:4, and 1:0.25 mixtures with EtOH (B:EtOH, mol:mol) were ranged from 3.82±0.20 to 5.00±0.37 μmol · g-dry cell wight (DCW)-1 · h-1. The degradation rate of T was the fastest in the 1:0.25 mixture (6.63±0.06 μmol · g-DCW-1 · h-1), and it was the lowest in the 1:4 mixture (4.41±0.04 μmol ·DCW-1 · h-1). The degradation rates of E were increased with increasing the addition amount of EtOH: The degradation rate of E was the highest in the 1:4 mixture (1.60±0.03 μmol · g-DCW-1 · h-1), and the rates were 1.42±0.06, 1.30±0.01, and 1.01±0.30 μmol · g-DCW-1 · h-1 in the 1:1, 1:0.25, 1.0 mixtures, respectively. In conclusion, the biodegradation of B, T, E by Rhodococcus sp. EH831 was not significantly inhibited by the co-existence of EtOH.
Abstract
The usage of ethanol (EtOH)-blended gasoline (gasohol), has been increasing in recent years. EtOH has influence on the distribution and biodegradation of aromatic compounds such as BTEX (benzene (B), toluene (T), ethylbenzene (B), and xylene (X)) that are gasoline compositions. In this study, the effect of EtOH on the aerobic biodegradation of B, T and E was investigated using a BTEX and EtOH-degrading bacterium, Rhodococcus sp. EH831. The degradation rates of B in the conditions of 1:1, 1:4, and 1:0.25 mixtures with EtOH (B:EtOH, mol:mol) were ranged from 3.82±0.20 to 5.00±0.37 μmol · g-dry cell wight (DCW)-1 · h-1. The degradation rate of T was the fastest in the 1:0.25 mixture (6.63±0.06 μmol · g-DCW-1 · h-1), and it was the lowest in the 1:4 mixture (4.41±0.04 μmol ·DCW-1 · h-1). The degradation rates of E were increased with increasing the addition amount of EtOH: The degradation rate of E was the highest in the 1:4 mixture (1.60±0.03 μmol · g-DCW-1 · h-1), and the rates were 1.42±0.06, 1.30±0.01, and 1.01±0.30 μmol · g-DCW-1 · h-1 in the 1:1, 1:0.25, 1.0 mixtures, respectively. In conclusion, the biodegradation of B, T, E by Rhodococcus sp. EH831 was not significantly inhibited by the co-existence of EtOH.
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