피톤치드 추출물의 항균 및 항산화 활성에 대한 연구
A Study on the Antimicrobial and Antioxidant Activity of Phytoncide Extract
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목적: 본 연구에서는 피톤치드 추출물 9종의 세포독성, 항산화력, 항균력의 생리활성을 규명하여 화장품 소재로서의 활용 가능성을연구하였다. 방법: 피톤치드 추출물과 관련된 천연물 9종을 선정한 후 추출하여 시료로 사용하였다. MTT assay를 통하여 안전성을확인하였으며, DPPH radical 소거 활성 평가를 통한 항산화 효능과 disc diffusion assay, minimum inhibitory concentration (MIC) 및 minimum bactericidal concentration (MBC)를 통한 항균 효과를 평가하였다. 결과: 피톤치드 추출물 9종 모두 3.00% 이하의 농도에서 fibroblast의 세포 독성에 대한 안전성을 확인하였다. 모든 피톤치드 추출물에서 DPPH radical 소거능력이 유사하였으며, 항산화 효능이 있음을 확인하였다. 항균 효능은 전체적으로 그람 음성균과 진균에 대해서는 강한 항균활성을 나타내지 못했으나, 그람 양성균에서 우수한 항균활성을 보였다. 산구절초추출물과 솔잣나무추출물은 그람 양성균에서만 항균 활성을 나타냈고, 향나무목부추출물은 그람 양성균과 효모에 대하여 우수한 항균 활성을 보였으며, 고본뿌리추출물은 세균보다는 진균에서 높은 항균 활성을 나타냈다. 결론: 이상의 연구 결과를 바탕으로 3.00% 이하의 농도에서 산구절초추출물, 솔잣나무추출물, 향나무목부추출물, 고본뿌리추출물에서 우수한 항균 및 항산화 효능을 확인하였으며, 합성 방부제나 항산화제를 대체하는 천연 성분의 화장품 소재로써활용 가능성이 높을 것으로 판단된다.
Abstract
Purpose: This study aims to confirm the physiological activity of cytotoxicity, antioxidant effects, and antibacterial effects of 9 kinds phytoncide extracts were investigated and the possibility of their application as cosmetic materials was studied. Methods: The 9 kinds natural products related to phytoncide extract were selected, extracted, and used as samples. Safety was confirmed through MTT assay, and antioxidant effect through DPPH radical scavenging activity evaluation and antibacterial effect through disc diffusion assay, minimum inhibitory concentration (MIC), and minimum bacteriological concentration (MBC) were evaluated. Results: All 9 kinds phytoncide extracts were confirmed to be safe against Fibroblast cytotoxicity at a concentration of 3.00% or less. The DPPH radical scavenging ability was similar in all phytoncide extracts, and it was confirmed that there was an antioxidant effect. The antibacterial effect did not show strong antibacterial activity against gram-negative bacteria, yeast and fungi as a whole, but it showed excellent antibacterial activity against gram-positive bacteria. Dendranthema zawadskii (Herb.) Tzvelev, Cupressus sempervirens showed antibacterial activity only on gram-positive bacteria. Juniperus chinensis showed excellent antibacterial activity against gram-positive bacteria, yeast. Angelica tenuissima Nakai showed high antibacterial activity against fungi rather than bacteria. Conclusion: These results show that excellent antibacterial and antioxidant effects were confirmed in Dendranthema zawadskii (Herb.) Tzvelev, Cupressus sempervirens, Juniperus chinensis and Angelica tenuissima Nakai at a concentration of 3.00% or less. It is judged that highly likely to be use as a cosmetic material with natural ingredients that replace synthetic preservatives or antioxidants.
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