폴리에틸렌 미세플라스틱의 임신 마우스 위내 투여 및 기도 점적에 따른 신생자 간독성 평가
Evaluation of Liver Toxicity of Neonates Following Intragastric Administration or Intratracheal Instillation of Polyethylene Microplatics to Pregnant Mice
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Background: Current research suggests that humans are exposed to microplastics through consumption of foods and beverages, the airway route, and a variety of other means. Objectives: We evaluated oxidative stress and inflammation from polyethylene microplastics (PE-MPs) in the neonatal liver through intragastric administration or intratracheal instillation in pregnant mice. Methods: PE-MPs were administered from gestational day 9 to postnatal day 7. The intragastric administration group (0.01 mg/mouse/day or 0.1 mg/mouse/day) and intratracheal instillation group (6 μg/mouse/day or 60 μg/mouse/day) of PE-MPs were administered. After sacrifice, the oxidative stress and inflammation of the neonatal livers were measured. Results: As a result of the oxidative stress caused by PE-MPs in the neonatal livers, glutathione peroxidase decreased in a concentration-dependent manner in the intragastric administration group compared to the control group and intratracheal instillation decreased in high concentration PE-MPs. The catalase level increased at high concentrations of intragastric administration and intratracheal instillation. To confirm the level of inflammation caused by PE-MPs, monocyte chemoattractant protein-1 and tumor necrosis factor- alpha were increased compared to the control group except for intratracheal intilation-high concentration PE- MPs. The C-reactive protein level was decreased by intragastric administration compared to the control group and intratracheal instillation was increased compared to the control group. Conclusions: Despite the difficulty in comparing the toxic intensity between intragastric administration and intratracheal instillation of PE-MPs, our study revealed that oxidative stress and inflammation were induced in the neonatal liver. However, it is necessary to evaluate the toxic effects of microplastics on various organs as well. Overall, the present study indicates that the evaluation of toxic effects of long-term microplastic exposure, potential of microplastic toxicity on next-generation offspring and toxicity mechanism in human should be considered for further investigations.
Abstract
Background: Current research suggests that humans are exposed to microplastics through consumption of foods and beverages, the airway route, and a variety of other means. Objectives: We evaluated oxidative stress and inflammation from polyethylene microplastics (PE-MPs) in the neonatal liver through intragastric administration or intratracheal instillation in pregnant mice. Methods: PE-MPs were administered from gestational day 9 to postnatal day 7. The intragastric administration group (0.01 mg/mouse/day or 0.1 mg/mouse/day) and intratracheal instillation group (6 μg/mouse/day or 60 μg/mouse/day) of PE-MPs were administered. After sacrifice, the oxidative stress and inflammation of the neonatal livers were measured. Results: As a result of the oxidative stress caused by PE-MPs in the neonatal livers, glutathione peroxidase decreased in a concentration-dependent manner in the intragastric administration group compared to the control group and intratracheal instillation decreased in high concentration PE-MPs. The catalase level increased at high concentrations of intragastric administration and intratracheal instillation. To confirm the level of inflammation caused by PE-MPs, monocyte chemoattractant protein-1 and tumor necrosis factor- alpha were increased compared to the control group except for intratracheal intilation-high concentration PE- MPs. The C-reactive protein level was decreased by intragastric administration compared to the control group and intratracheal instillation was increased compared to the control group. Conclusions: Despite the difficulty in comparing the toxic intensity between intragastric administration and intratracheal instillation of PE-MPs, our study revealed that oxidative stress and inflammation were induced in the neonatal liver. However, it is necessary to evaluate the toxic effects of microplastics on various organs as well. Overall, the present study indicates that the evaluation of toxic effects of long-term microplastic exposure, potential of microplastic toxicity on next-generation offspring and toxicity mechanism in human should be considered for further investigations.
참고문헌 (45)
- Doyle MJ, "lastic particles in coastal pelagic ecosystems of the Northeast Pacific ocean", Mar Environ Res, 71(1), pp.41~52 (2011)
- Rochman CM, "Policy: classify plastic waste as hazardous", Nature, 494(7436), pp.169~171 (2013)
- Ebere EC, "Uptake of microplastics by plant: a reason to worry or to be happy?", World Sci News, 131, pp.256~267 (2019)
- Hirt N, "Immunotoxicity and intestinal effects of nano- and microplastics: a review of the literature", Part Fibre Toxicol, 17(1), pp.57~ (2020)
- da Costa JP, "(Nano)plastics in the environment - sources, fates and effects", Sci Total Environ, 566-567, pp.15~26 (2016)
- Andrady AL, "Microplastics in the marine environment", Mar Pollut Bull, 62(8), pp.1596~1605 (2011)
- Enyoh CE, "Microplastics exposure routes and toxicity studies to ecosystems: an overview", Environ Anal Health Toxicol, 35(1), pp.e2020004~ (2020)
- Carbery M, "Trophic transfer of microplastics and mixed contaminants in the marine food web and implications for human health", Environ Int, 115, pp.400~409 (2018)
- Rahman A, "Potential human health risks due to environmental exposure to nano- and microplastics and knowledge gaps: a scoping review", Sci Total Environ, 757, pp.143872~ (2021)
- Abbasi S, "Microplastics in different tissues of fish and prawn from the Musa Estuary, Persian Gulf", Chemosphere, 205, pp.80~87 (2018)
- Prata J, "Airborne microplastics: consequences to human health?", Environ Pollut, 234, pp.115~126 (2018)
- Yang W, "The combined toxicity influence of microplastics and nonylphenol on microalgae Chlorella pyrenoidosa", Ecotoxicol Environ Saf, 195, pp.110484~ (2020)
- Gao H, "Bidesmoside triterpenoid glycosides from Stauntonia chinensis and relationship to anti-inflammation", Phytochemistry, 70(6), pp.795~806 (2009)
- Deng Y, "Tissue accumulation of microplastics in mice and biomarker responses suggest widespread health risks of exposure", Sci Rep, 7, pp.46687~ (2017)
- Lu Y, "Uptake and accumulation of polystyrene microplastics in zebrafish (Danio rerio) and toxic effects in liver", Environ Sci Technol, 50(7), pp.4054~4060 (2016)
- Lee S, "Toxicity study and quantitative evaluation of polyethylene microplastics in ICR mice", Polymers (Basel), 14(3), pp.402~ (2022)
- Li B, "Polyethylene microplastics affect the distribution of gut microbiota and inflammation development in mice", Chemosphere, 244, pp.125492~ (2020)
- 송영민, "폴리에틸렌 미세플라스틱의 임신 마우스 위내투여에 따른 모체 및 신생자 독성평가", 한국환경보건학회지, 47(5), pp.446~453 (2021), 한국환경보건학회[DOI]
- Wright SL, "Plastic and human health: a micro issue?", Environ Sci Technol, 51(12), pp.6634~6647 (2017)
- Han YoungHoon, "No prominent toxicity of polyethylene microplastics observed in neonatal mice following intratracheal instillation to dams during gestational and neonatal period", 한국독성학회지, 37(4), pp.443~450 (2021), 한국독성학회[DOI]
- Han YoungHoon, "Erratum : No prominent toxicity of polyethylene microplastics observed in neonatal mice following intratracheal instillation to dams during gestational and neonatal period", 한국독성학회지, 38(1), pp.111~111 (2022), 한국독성학회[DOI]
- Park TJ, "Occurrence of microplastics in the Han River and riverine fish in South Korea", Sci Total Environ, 708, pp.134535~ (2020)
- Bendtsen KM, "Airport emission particles: exposure characterization and toxicity following intratracheal instillation in mice", Part Fibre Toxicol, 16(1), pp.23~ (2019)
- Yin K, "A comparative review of microplastics and nanoplastics: toxicity hazards on digestive, reproductive and nervous system", Sci Total Environ, 774(20), pp.145758~ (2021)
- Dris R, "A first overview of textile fibers, including microplastics, in indoor and outdoor environments", Environ Pollut, 221, pp.453~458 (2017)
- Vianello A, "Simulating human exposure to indoor airborne microplastics using a Breathing Thermal Manikin", Sci Rep, 9(1), pp.8670~ (2019)
- aggio C, "Cytotoxicity, haemolymphatic parameters, and oxidative stress following exposure to sub-lethal concentrations of quaternium-15 in Mytilus galloprovincialis", Aquat Toxicol, 180, pp.258~265 (2016)
- Prokić MD, "Ecotoxicological effects of microplastics: examination of biomarkers, current state and future perspectives", Trends Analyt Chem, 111, pp.37~46 (2019)
- 김한빛, "Propolis Inhibits UVA-Induced Apoptosis of Human Keratinocyte HaCaT Cells by Scavenging ROS", 한국독성학회지, 32(4), pp.345~351 (2016), 한국독성학회
- Oliveira P, "Effects of microplastics and mercury in the freshwater bivalve Corbicula fluminea (Müller, 1774): filtration rate, biochemical biomarkers and mercury bioconcentration", Ecotoxicol Environ Saf, 164, pp.155~163 (2018)
- Yu P, "Accumulation of polystyrene microplastics in juvenile Eriocheir sinensis and oxidative stress effects in the liver", Aquat Toxicol, 200, pp.28~36 (2018)
- Davies KJ, "Adaptive homeostasis", Mol Aspects Med, 49, pp.1~7 (2016)
- Jin H, "Polystyrene microplastics induced male reproductive toxicity in mice", J Hazard Mater, 401, pp.123430~ (2021)
- Marra F, "Chemokines in liver inflammation and fibrosis", Front Biosci, 7, pp.d1899~1914 (2002)
- Zamara E, "Prevention of severe toxic liver injury and oxidative stress in MCP-1-deficient mice", J Hepatol, 46(2), pp.230~238 (2007)
- Li S, "Keap1-Nrf2 pathway upregulation via hydrogen sulfide mitigates polystyrene microplastics induced-hepatotoxic effects", J Hazard Mater, 402, pp.123933~ (2021)
- Choi D, "In vitro toxicity from a physical perspective of polyethylene microplastics based on statistical curvature change analysis", Sci Total Environ, 752, pp.142242~ (2021)
- Yang G, "Elevated serum IL-11, TNF α, and VEGF expressions contribute to the pathophysiology of hypertensive intracerebral hemorrhage (HICH)", Neurol Sci, 37(8), pp.1253~1259 (2016)
- Sproston NR, "Role of C-reactive protein at sites of inflammation and infection", Front Immunol, 9, pp.754~ (2018)
- Salehifar E, "Role of C-reactive protein and tumor necrosis factor-alpha in differentiating between ventilator-associated pneumonia and systemic inflammatory response syndrome without infectious etiology", Tanaffos, 15(4), pp.205~212 (2016)
- Pepys MB, "C-reactive protein: a critical update", J Clin Invest, 111(12), pp.1805~1812 (2003)
- Pepys MB, "C-reactive protein: a critical update", Erratum in: J Clin Invest, 112(2), pp.299~ (2003)
- Dröge W, "Free radicals in the physiological control of cell function", Physiol Rev, 82(1), pp.47~95 (2002)
- Hussain SP, "Radical causes of cancer", Nat Rev Cancer, 3(4), pp.276~285 (2003)
- Park EJ, "Repeated- oral dose toxicity of polyethylene microplastics and the possible implications on reproduction and development of the next generation", Toxicol Lett, 324, pp.75~85 (2020)