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조피볼락(Sebastes schlegelii)의 혈액성상, 혈장성분 및 항산화 반응에 미치는 미세플라스틱의 독성 영향

Toxic Effects of Microplastic on Hematological Parameters, Plasma Components, and Antioxidant Responses in the Korean Rockfish Sebastes schlegelii

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초록

This study aimed to evaluate the effects of polyethylene microplastic (PE-MPs) via measuring the growth performance, hematological parameters, and antioxidant responses in Korean rockfish Sebastes schlegelii exposed to waterborne polyethylene microplastic with dimensions of 22–71 μm. S. schlegelii (mean weight, 34.55±5.82 g; mean length, 12.59±0.79 cm) were exposed to PE-MPs at concentrations of 0, 400, 800, 1,600 and 3,200 μg/L for 10 and 20 days. PE-MPs significantly affected growth performance, hematological parameters, plasma components, and antioxidant responses in a concentration-dependent manner. At a concentration ≥1,600 μg/L, PE-MPs significantly decreased body weight gain and specific growth rate, and significantly increased the hepatosomatic index. Hematological parameters showed a significant decrease in total red blood cell count and hemoglobin levels. Plasma components showed a significant increase in glucose, aspartate aminotransferase, and alanine transaminase levels, whereas total protein, calcium, and magnesium levels significantly decreased. Exposure to ≥1,600 μg/L PE-MPs also induced reactive oxygen species generation in the gill and liver, significantly increasing superoxide dismutase and catalase activity. These findings suggest that exposure to ≥1,600 μg/L PE-MPs could significantly change growth performance, hematological parameters, plasma components, and antioxidant responses, resulting in physiological toxicity.

Abstract

This study aimed to evaluate the effects of polyethylene microplastic (PE-MPs) via measuring the growth performance, hematological parameters, and antioxidant responses in Korean rockfish Sebastes schlegelii exposed to waterborne polyethylene microplastic with dimensions of 22–71 μm. S. schlegelii (mean weight, 34.55±5.82 g; mean length, 12.59±0.79 cm) were exposed to PE-MPs at concentrations of 0, 400, 800, 1,600 and 3,200 μg/L for 10 and 20 days. PE-MPs significantly affected growth performance, hematological parameters, plasma components, and antioxidant responses in a concentration-dependent manner. At a concentration ≥1,600 μg/L, PE-MPs significantly decreased body weight gain and specific growth rate, and significantly increased the hepatosomatic index. Hematological parameters showed a significant decrease in total red blood cell count and hemoglobin levels. Plasma components showed a significant increase in glucose, aspartate aminotransferase, and alanine transaminase levels, whereas total protein, calcium, and magnesium levels significantly decreased. Exposure to ≥1,600 μg/L PE-MPs also induced reactive oxygen species generation in the gill and liver, significantly increasing superoxide dismutase and catalase activity. These findings suggest that exposure to ≥1,600 μg/L PE-MPs could significantly change growth performance, hematological parameters, plasma components, and antioxidant responses, resulting in physiological toxicity.

참고문헌 (63)

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