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홍화인 추출물이 골 형성에 미치는 영향에 관한 실험실적 연구

A study of safflower seed extracts on bone formation in vitro

최호철|이성진|피성희|유형근|신형식|선기종|송제봉
대한치주과학회 (The Korean Academy Of Periodontology)|2005.06|Journal of Periodontal & Implant Science|제35권 제2호|pp. 461-474

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참고문헌

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Abstract

The ultimate goal of periodontal therapy is the regeneration of periodontal tissue and the repair of function. For more than a decade there have been many efforts to develop ma- terials and methods of treatment to promote periodontal tissue regeneration. Recently many efforts are concentrated on the regeneration potential of material used in traditional medicine. Safflower(Carthamus tinctorius L.) seed extract(SSE) have long clinically used in Korea to promote bone formation and prevent osteoporosis. The purpose of this study was to examine the effects of SSE on bone formation in human osteoblastic cell line. Human fetal osteoblastic cell line(hFOB 1.19) was cultured with DMEM and SSE(1㎍/㎖, 10㎍/㎖, 100㎍/㎖, 1mg/㎖) at 34℃ with 5% CO2 in 100% humidity. The proliferation, differentiation of the cell was evaluated by several experiments. Cell proliferation was significantly increased at 10㎍/㎖, 100㎍/㎖, 1mg/㎖ of SSE after 3 and 7 days incubation(p＜0.05). Cell spreading assay was significantly increased at 100㎍/㎖ of SSE after 3 days and 1㎍/㎖, 10㎍/㎖, 100㎍/㎖, 1mg/㎖ of SSE after 7 days(p＜0.05). Alkaline Phosphatase(ALP) level was significantly increased in 10㎍/㎖, 100㎍/㎖ of SSE(p＜0.05). Collagen synthesis was significantly increased at 10㎍/㎖, 100㎍/㎖, 1mg/㎖ of SSE(p＜0.05). A quantified calcium accumulation was significantly increased at 10㎍/㎖, 100㎍/㎖ of SSE(p＜0.05). ALP and osteocalcin mRNA was expressed in 100㎍/㎖ of SSE by RT-PCR. These results indicate that SSE are capable of increasing osteoblasts mineralization and may play an important role in bone formation.

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랫드의 두개골세포 활성 및 mRNA 발현에 미치는 홍화자의 영향김태웅, 황귀서대한예방한의학회 · 대한예방한의학회지2007 · 제11권 제1호홍화, 홍화씨 추출물이 MC3T3E1 세포의 골분화 과정에 미치는 영향신선미, 유성률대한한방내과학회 · 대한한방내과학회지2015 · 제36권 제4호홍화씨 추출물이 MC3T3-E1 조골세포의 증식과 분화에 미치는 영향김도경, 김수관, 김진수, 김춘성, 모신엽, 문소라, 박민경, 박선영, 오지수, 이동설, 이슬아, 이승용, 조광희, 조선호, 최미숙치의학연구원 · Oral Biology Research2013 · 제37권 제2호속단의 dichloromethane 분획물이 태아골모세포의 골형성 유도에 미치는 효과김윤철, 신형식, 유형근, 이영준, 최희인대한치주과학회 · Journal of Periodontal & Implant Science2003 · 제33권 제2호Effects of Achyranthes Radix Extracts on Osteoblasts and Osteocalsts고선일, 김강용, 김세원, 김정근대한구강생물학회 · International Journal of Oral Biology2005 · 제30권 제2호수종의 생약제제가 human fetal osteoblasts의 염기성 인산분해 효소 활성에 미치는 영향신형식, 유형근, 이명구, 최희인대한치주과학회 · Journal of Periodontal & Implant Science2003 · 제33권 제1호

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