본문 바로가기
KERD
논문KCI 등재

백서 태자 두개관세포에서 인삼 사포닌에 의한 MMP-13 mRNA 발현 억제

Inhibition of MMP-13 mRNA expression by ginseng saponin in fetal rat calvarial cells

1

조회

0

다운로드

0

인용

0

참고문헌

주제분류치의학

초록

Abstract

There is a potential role of collagenase-3 in alveolar bone loss and periodontal disease progression, we need to develope or find chemotherapeutic drugs or herbal agents which may regulate the expression of MMP-13. Ginseng saponin, one of the major components of Korea ginseng(panax ginseng) root, has many various biologic effects, such as cytotoxic effect, tumoricidal effects, cytokine regulations, and protein biosynthesis effect. The purpose of this study was to determine the effects of Korea red ginseng saponin on MMP-13 gene expression in osteoblasts. The experimental groups were cultured with ginseng saponin in concentration of 1.0, 10, 25, 50, 100, 250 and 500㎍/㎖ for MTT assay. Primary rat calvarial cells were pre-treated for 1 hour with ginseng saponin(100 ㎍/㎖) and then stimulated with IL-1β(1.0ng/㎖) and PTH (10 nM). MMP-13 gene expression was evaluated by RT-PCR. The results were as follows: Ginseng saponin was cytotoxic to osteoblast at concentration exceeding 250㎍/㎖ for longer than 24 hours in tissue culture(p<0.01). In RT-PCR analysis, steady state MMP-13 mRNA levels were increased approximately 350% by IL-1β, and 400% by PTH when normalized to untreated control. IL-1β-indued MMP-13 mRNA expression was reduced 50% by pre- treatment with ginseng saponin. But ginseng saponin didn't inhibit MMP-13 expression from PTH stimulated cells. This results suggest that ginseng saponin inhibit IL-1β-indued MMP-13 mRNA expres- sion.

참고문헌 (41)

  1. "Funder- mentals of periodontics", pp.61~107 (1996), Quintessence publishing Co
  2. "Gingival fibroblasts degrade type I collagen films when stimulated with tumor necrosis factor and interleukin-1 Evidence that breakdown is mediated by metallopro- teinases", pp.207~213 (1989)
  3. "Ad- vances in understanding cell interactions in tissue resorption Relevance to the pa- thogenesis of periodontal diseases and a new hypothesis", pp.239~250 (1991)
  4. "Cy- tokine-induced endogenous procollagenase stored in the extracellular matrix of soft connective tissue results in a burst of collagen breakdown following its activation", 31, pp.483~488 (1996)
  5. "Role of matrix metalloproteinases in human periodon- tal diseases", 64, pp.474~484 (1993)
  6. "Mechanism of connective tissue matrix destruction in periodontitis", 14, pp.144~157 (1997)
  7. "Cytokine regulation of metalloproteinase expression", 53, pp.288~295 (1993)
  8. "Effects of TNF alpha and prostaglandin E2 on the expression of MMPs in human periodontal ligament fibroblasts", pp.167~76 (2002)
  9. "Compara- tive immunolocalization studies of collagenase 1 and collagenase 3 production in the rheumatoid lesion and by human chondrocytes and synoviocytes in vitro", pp.64~70 (1998)
  10. "Transforming growth factor β1 inhibits collagenase 3 expression by transcriptional and post-transcriptional mechanisms in osteoblast cultures", 170, pp.145~152 (1997)
  11. "Regulation of matrix metallopro- teinases by interleukin-1 and -6 in mouse calvaria Association of MMP induction with bone resorption", kusa(k), pp.1338~2 13 1345
  12. "A matrix metalloproteinase inhibitor reduces bone-type collagen degradation fragments and specific collagenases in gingival crevicular fluid during adult periodontitis", 46, pp.310~319 (1997)
  13. "molecular forms and levels in gingival crevicular fluid and immunolocalisation in gingival tissue", pp.224~2 8 3 13 232 (2002)
  14. "Collagena- ses in different categories of periimplant vertical bone loss", 79, pp.1870~1873 (2000)
  15. "-type matrix me- talloproteinases in human adult periodontitis gingiva", pp.100~109 (1995)
  16. "Regulation of cartilage collagenase by doxycycline", 28, pp.835~842 (2001)
  17. ":A combination of a chemically modified doxycycline and a bisphosphonate synergistically inhibits endotoxin-induced periodontal breakdown in rats", 72, pp.1069~77 (2001)
  18. ":Antibiotic resistance profile of the subgingival microbiota following systemic or local tetracycline therapy", 31, pp.420~7 (2004)
  19. "The inhibitory effect of palnt extracts on the collagenolytic activity and cytotoxicity of human gingival fibroblasts by Porphyromonas gingivalis crude enzyme", pp.1~7 (1991)
  20. "Magnolol과 Honokiol이 항균,교원질분해효소,세포독성 및 cytokine 생산에 미치는 영향", 23, pp.145~158 (1993)
  21. "황련이 lipopolysaccharide를 처리한 치주인대 세포의 세포활성 및 IL-6 생산에 미치는 영향", 26, pp.641~654 (1996)
  22. "고려인삼의 효능연구 최종 결과 보고서", 한국담배인삼공사 1996
  23. "고려인삼의 효능연구 최종 결과 보고서", 한국담배인삼공사 1997
  24. "홍삼의 약리작용", 25, pp.2~10 (2001)
  25. "Immunomodulatory response induced by ginseng", 27, pp.115~119 (2003)
  26. "인삼성분의 임상적 효능과 생화학적 작용기전", 25, pp.19~25 (2001)
  27. "Further biochemical and molecular cha- racterization of primary rat parietal bone cell culture", pp.401~408
  28. "In- sulinlike growth factor I inhibits the transcription of collagenase 3 in osteoblast cultures", 67, pp.176~183 (1997)
  29. "Transforming growth factor beta 1 inhibits collagenase 3 expression by transcriptional and post-transcriptional me- chanisms in osteoblast cultures", 170, pp.145~152 (1997)
  30. "Regulation of collagenase-3 by bone morphogenetic protein-2 in bone cell cultures", 138, pp.1035~1040 (1997)
  31. "Parathyroid hormone increases the expression level of matrix metaloprotei- nase-13 in vivo", 19, pp.207~212 (2001)
  32. "Inter- leukin-1 induction of collagenase 3 gene expression in chondrocytes requires p38,JNK,and nuclear factor κB", 43, pp.801~811 (2000)
  33. "Ma- trix metalloproteinase and proinflamma- tory cytokine production by chondrocy- tes of human osteoarthritic cartilage associations with degenerative changes", pp.585~594
  34. "Regulat- ed expression of collagenases MMP-1 and-11 by human corneal epithelial cells", pp.2928~2936 (2003)
  35. "Glucocorticoid receptor-induced down- regulation of MMP-9 by ginseng components PD and PT contributes to inhibition of the invasive capacity of HT- 1080 human fibrosarcoma cells", pp.476~83 (1999)
  36. ":Inhibitory effect of ginsenoside-Rb2 on invasiveness of uterine endometrial cancer cells to the basement membrane", 22, pp.339~41 (2001)
  37. "Glucocor- ticoid-mediated suppression of cytokine- induced matrix metalloproteinase-9 expression in rat mesangial cells involvement of nuclear factor-kappaB and Ets transcription factors", pp.1752~1766 (2002)
  38. "The effect of Korean red ginseng saponin on the growth and differentiation of human periodontal ligament cell in culture", 25, pp.45~55 (1995)
  39. "Role of transforming growth factor-β pathway in the mechanism of wound healing by saponin from Ginseng Radix rubra", 125, pp.255~262 (1998)
  40. "proliferation rates and vinculin assembly of bovine osteoblasts cultured on different pre-coated artificial substrates", pp.301~307 (1998)
  41. "proliferation rates and vinculin assembly of bovine osteoblasts cultured on different pre-coated artificial substrates", pp.301~307 (1998)

관련 논문AI추천

이 간행물의 다른 논문 (20건)