암세포주에 대한 Gentisic Acid의 선택적 세포독성

Abstract

The purpose of this study was to investigate selective cytotoxicities of gentisic acid in cancer celllines, Gentisic acid is aromatic phenolic compounds which had two hydroxyl and a carboxyl radical. The cytotoxiciψ of gentisic acid against N퍼3T3 fibroblasts (NIH3T3), human gingiv외 fibroblasts (HGF), human skin melanoma cells (SK-MEL-3) and human oral epithelioid carcinoma cells (KB) were evaluated by the MTT assay for cell viability and XTT assay for cell adbesion activity, The microscopic study was canied out to observe morphological changes in four kinds of cells, The results were as follows : 1. The cytotoxicity of gentisic acid against NIH3T3 was statistically significant in 50 메-1 or 100 μ1M gentisic acid containing m뼈1a， IC때 of gentisic acid itself by the MTT and XTT assay were 1,4æ.8 μ1M and 1,245.5 마Æ， respectively. 2. The cytotoxicity of gentisic acid against HGF was statistically not significant. lCÆIJ of gentisic acid itself by the MTT and XTI assay were 1,090‘0 마Æ and 1,130,6 pM, respectively. 3. The cytotoxicity of gentisic acid ag띠nst SK-MEL-3 was statistically significant in 100 μ1M gentisic acid containing m때a. IC~ of gentisic acid i잉elf by the MTT and XTT assay were 779.9 pM and 831.9 μM. respectively. 4‘ ’fhe cytoto싱city of gentisic acid against KB was statistically significant in 엉 μ1M gentisic acid containing media‘ ICf/l of gentisic acid itself by the MTT and XTT assay were 182.9 μ1M and 146.2 pM, res않ctively. 5. In morphological changes, NIH3T3 and HGF treated with gentisic acid itself were slightly decreased and cell number of SK-MEL-3 and KB were decreased by dose dependent manner and cell type was transformed into round shape. The morphological changes in KB were the most severe These results suggests that gentisic acid have selective cytotoxicity in cancer cell lines.