본문 바로가기
KERD
논문KCI 등재

加味逍遙散의 抗酸化 效果 및 神經細胞 保護效果

Antioxidant and Neuroprotective Effects of Gamisoyo-san

이진무|장준복|이창훈|조정훈|이경섭|이성한
대한한방부인과학회 (The Society Of Oriental Obstetrics & Gynecology)|2010.08|대한한방부인과학회지 (The Journal of Oriental Obstetrics ＆ Gynecology)|제23권 제3호|pp. 1-13

2

조회

0

다운로드

4

인용

0

참고문헌

키워드

MenopauseGamisoyo-sanAntioxidantsHerbmedNeuroprotective agents
주제분류한의학

초록

Purpose: These studies were undertaken to evaluate the anti-oxidative and neuroprotective effects of Gamisoyo-san(GMSYS). Materials and Methods: We studied the antioxidant effects of GMSYS by assessing the DPPH free radical and the ABTS radical cation inhibition activities, the total polyphenolic contents(TPC). To evaluate the effects of GMSYS in the human neuroblastoma cells, we measured the cell viabilities in SH-SY5Y cells treated with GMSYS. Then we observed the protective effects of GMSYS against 6-OHDA induced neurotoxicity in SH-SY5Y cells. To confirm the neuroprotective effects of GMSYS in the primary culture of mesencephalic dopaminergic cells, we counted the TH-immunopositive cells and measured the NO and TNF-α after the treatment of GMSYS and 6-OHDA. Results: The DPPH free radical and the ABTS radical cation inhibition activities were increased in a dose dependent manner and the IC50 were 133.60㎍/㎖ and 106.20㎍/㎖, respectively. The TPC was 0.78%. There were no differences between the various concentrations of GMSYS and the control in the cell viability of SH-SY5Y cells. The neuroprotective effects of GMSYS were shown in the co-treatment group at the low concentrations of 25㎍/㎖ and the post-treatment group at all concentrations. After the treatment of GMSYS and 6-OHDA in the primary culture of dopaminergic cells, the TH-immunopositive cells were significantly increased in 0.2㎍/㎖ of GMSYS than the 6-OHDA group. The NO and TNF-α were significantly decreased in 0.2㎍/㎖ of GMSYS than the 6-OHDA group. Conclusions: This study shows that GMSYS has the antioxidant and neuroprotective effects, especially in the mesencephalic dopaminergic cells. We suggest that GMSYS could be useful for the treatment of postmenopausal depression related with the degeneration of dopamine neuron.

Abstract

Purpose: These studies were undertaken to evaluate the anti-oxidative and neuroprotective effects of Gamisoyo-san(GMSYS). Materials and Methods: We studied the antioxidant effects of GMSYS by assessing the DPPH free radical and the ABTS radical cation inhibition activities, the total polyphenolic contents(TPC). To evaluate the effects of GMSYS in the human neuroblastoma cells, we measured the cell viabilities in SH-SY5Y cells treated with GMSYS. Then we observed the protective effects of GMSYS against 6-OHDA induced neurotoxicity in SH-SY5Y cells. To confirm the neuroprotective effects of GMSYS in the primary culture of mesencephalic dopaminergic cells, we counted the TH-immunopositive cells and measured the NO and TNF-α after the treatment of GMSYS and 6-OHDA. Results: The DPPH free radical and the ABTS radical cation inhibition activities were increased in a dose dependent manner and the IC50 were 133.60㎍/㎖ and 106.20㎍/㎖, respectively. The TPC was 0.78%. There were no differences between the various concentrations of GMSYS and the control in the cell viability of SH-SY5Y cells. The neuroprotective effects of GMSYS were shown in the co-treatment group at the low concentrations of 25㎍/㎖ and the post-treatment group at all concentrations. After the treatment of GMSYS and 6-OHDA in the primary culture of dopaminergic cells, the TH-immunopositive cells were significantly increased in 0.2㎍/㎖ of GMSYS than the 6-OHDA group. The NO and TNF-α were significantly decreased in 0.2㎍/㎖ of GMSYS than the 6-OHDA group. Conclusions: This study shows that GMSYS has the antioxidant and neuroprotective effects, especially in the mesencephalic dopaminergic cells. We suggest that GMSYS could be useful for the treatment of postmenopausal depression related with the degeneration of dopamine neuron.

참고문헌 (47)

  1. Farage MA, "Cognitive, sensory and emotional changes associated with the menstrual cycle: a review", Arch Gynecol Obstet, 278(4), pp.299~307 (2008)
  2. Nelson HD, "Menopause", Lancet, 371(9614), pp.760~770 (2008)
  3. Steinberg EM, "A cross-sectional evaluation of perimenopausal depression", J Clin Psychiatry, 69(6), pp.973~980 (2008)
  4. Craig MC, "Estrogen: effects on normal brain function and neuropsychiatric disorders", Climacteric, 10, pp.97~104 (2007)
  5. Watson CS, "Estradiol effects on the dopamine transporter-protein levels, subcellular location and function", J Mol Signal, 1, pp.1~14 (2006)
  6. Gardiner SA, "Pilot study on the effect of estrogen replacement therapy on brain dopamine transporter availability in healthy, postmenopausal women", Am J Geriatr Psychiatry, 12(6), pp.621~630 (2004)
  7. Craig MC, "Effect of long-term estrogen therapy on dopaminergic responsivity in post-menopausal women: a preliminary study", Psychoneuroendocrinology, 29(10), pp.1309~1316 (2004)
  8. 陳師文, "太平惠民和劑局方", pp.308~ (1985), 人民衛生出版社
  9. 薛己 注, "校注婦人良方", pp.419~423 (1991), 江西人民出版社
  10. 金在瑩, "丹梔逍遙散에 關한 文獻的 考察", 大田大學校 韓醫學硏究所 論文集, 7(2), pp.531~551 (1999)
  11. 李錫駿, "柳氏逍遙散과 柳氏歸脾湯의 抗스트레스 效果에 關한 臨床的 硏究", 東醫神經精神科學會誌, 5(1), pp.41~60 (1994)
  12. 崔銀洙, "加味逍遙散의 效能에 關한 實驗的 硏究", 大韓韓方婦人科學會誌, 9(1), pp.41~53 (1996)
  13. 홍성은, "逍遙散이 생쥐에 Immobilization Stress 負荷 後 血中 免疫能系에 미치는 影響", 대한한방부인과학회지, 16(3), pp.101~127 (2003), 대한한방부인과학회
  14. 황귀서, "단치소요산이 구속 스트레스를 가한 흰쥐의 뇌내 catecholamine 함량변화에 미치는 영향", Kor J Environ Toxicol, 13(3), pp.143~149 (1998)
  15. 이정호, "逍遙散과 淸肝逍遙散이 스트레스 생쥐의 腦部位別 monoamines 含量에 미치는 影響", 東醫神經精神科學會誌, 9(2), pp.1~18 (1998)
  16. 金潤相, "逍遙散이 卵巢摘出 흰쥐에 미치는 抗憂鬱效果", 大韓韓方婦人科學會誌, 14(2), pp.69~84 (2001)
  17. 오승희, "逍遙散 藥鍼이 난소적출 흰쥐의 抗스트레스 作用에 미치는 影響", 대한침구학회지, 22(1), pp.117~130 (2005), 대한침구학회
  18. 대한민국 통계청, "2005년 생명표" (2006)
  19. 대한민국 통계청, "2001년 국민건강⋅영양조사" (2002)
  20. Berg JA, "Menopausal symptom perception and severity: results from a screening questionnaire", J Clin Nurs, 17(7), pp.940~948 (2008)
  21. Woods NF, "Pathways to depressed mood for midlife women: observations from the Seattle Midlife Women's Health Study", Res Nurs Health, 29(2), pp.119~129 (1997)
  22. Avis NE, "Longitudinal study of hormone levels and depression among women transitioning through menopause", Climacteric, 4(3), pp.243~249 (2001)
  23. Bromberger JT, "Psychologic distress and natural menopause: a multiethnic community study", Am J Public Health, 91(9), pp.1435~1442 (2001)
  24. Dennerstein L, "The unique challenges of managing depression in mid-life women", World Psychiatry, 7(3), pp.137~142 (2008)
  25. Pae CU, "Effectiveness of antidepressant treatments in pre- menopausal versus post-menopausal women: a pilot study on differential effects of sex hormones on antidepressant effects", Biomed Pharmacother, 30, pp.1~8 (2008)
  26. Ng F, "Oxidative stress in psychiatric disorders: evidence base and therapeutic implications", Int J Neuropsychopharmacol, 11(6), pp.851~876 (2008)
  27. Reynolds A, "Oxidative stress and the pathogenesis of neurodegenerative disorders", Int Rev Neurobiol, 82, pp.297~325 (2007)
  28. Gredilla R, "Differential age- related changes in mitochondrial DNA repair activities in mouse brain regions", Neurobiol Aging, 11, pp.1~10 (2008)
  29. 中山醫學院, "中醫方劑學選講", pp.173~181 (1983), 廣東科學技術出版社
  30. 趙漢栢, "婦人科에 活用되고 있는 逍遙散에 對한 硏究", 大韓韓方婦人科學會誌, 13(2), pp.265~277 (2000)
  31. 崔昇勳, "丹梔逍遙散이 白鼠의 甲狀腺 損傷에 미치는 影響", 대전대학교 대학원, ~
  32. 金昭延, "加味逍遙散이 스트레스로 因한 白鼠의 乳汁過多分泌에 미치는 影響", 경산대학교 대학원, ~
  33. 강솔, "逍遙散이 白鼠의 排卵에 미치는 影響", 大韓韓方婦人科學會誌, 13(1), pp.111~126 (2000)
  34. 박찬수, "加味逍遙散이 난소적출로 유발된 흰 쥐의 골다공증에 미치는 영향", 대한한방부인과학회지, 21(1), pp.1~15 (2008), 대한한방부인과학회
  35. 김은섭, "逍遙散加減化裁의 멜라닌 생성 억제와작용기전에 관한 연구", 대한한방부인과학회지, 21(1), pp.83~98 (2008), 대한한방부인과학회
  36. 신경숙, "丹梔逍遙散이 갱년기 장애에 미치는 효능에 관한 임상보고", 대한한방부인과학회지, 16(4), pp.77~82 (2003), 대한한방부인과학회
  37. Nishizawa M, "Non-reductive scavenging of 1,1-Diphenyl-2- picrylhydrazyl(DPPH) by peroxyradical: a useful method for quantitative analysis of peroxyradical", Chem Pharm Bull, 53(6), pp.714~716 (2005)
  38. Kim HJ, "Vanillic acid glycoside and quinic acid derivatives from Gradeniae Fructus", J Nat Prod, 69(4), pp.600~603 (2006)
  39. Masuda Y, "Antioxidant properties of gingerol related compounds from ginger", Biofactors, 21(1), pp.293~296 (2004)
  40. Sroka Z, "Antiradical and anti-H202 properties of polyphenolic compounds from and aqueous peppermint extract", Z Naturforshc, 60(11), pp.826~832 (2005)
  41. Kang TH, "Protective effect of jakyak-gamcho-tang extract and its constituents against t-BHP -induced oxidative damage in HT22 cells", Am J Chin Med, 33(2), pp.181~189 (2005)
  42. Re R, "Antioxidant activity applying an improved ABTS radical cation decolorization assay", Free Radic Biol Med, 26(9), pp.1231~1237 (1999)
  43. Appel H, "Limitations of Folin assays of foliar phenolics in ecological studies", J Chem Ecol, 27(4), pp.761~778 (2001)
  44. Gomez-Lazaro M, "6-hydroxydopamine (6-OHDA) induces Drp-1-dependent mitochondrial fragmentation in SH- SY5Y cells", Free Radic Biol Med, 44(11), pp.1960~1969 (2008)
  45. Carvour M, "Chronic low-dose oxidative stress induces caspase-3- dependent PKCδ proteolytic activation and apoptosis in a cell culture model of dopaminergic neurodegeneration", Ann N Y Acad Sci, 1139, pp.197~205 (2008)
  46. Kumral A, "Neuroprotective effect of the peptides ADNF-9 and NAP on hypoxic-ischemic brain injury in neonatal rats", Brain Res, 1115(1), pp.169~178 (2006)
  47. O'Callaghan JP, "Defining "neuroinflammation"", Ann NY Acad Sci, 1139, pp.318~330 (2008)

관련 논문AI추천

分心氣飮의 도파민 세포 보호 효과김로사, 이경섭, 이진무, 이창훈, 장준복, 조정훈대한한방부인과학회 · 대한한방부인과학회지2009 · 제22권 제2호歸脾湯과 歸脾湯加味方의 항산화 효과 및 6-Hydroxydopamine에 대한 PC12 세포 보호효과 비교연구김종우, 오명숙, 임재환, 정선용, 조성훈, 황의완대한한방신경정신과학회 · 동의신경정신과학회지2009 · 제20권 제1호六鬱湯의 산화적 스트레스에 대한 뇌세포 보호효과이경섭, 이진무, 이창훈, 장준복, 정선형, 조정훈대한한방부인과학회 · 대한한방부인과학회지2009 · 제22권 제1호人蔘養營湯의 산화적 stress에 대한 뇌세포 보호효과김승현, 이경섭, 이진무, 이창훈, 장준복, 조정훈대한한방부인과학회 · 대한한방부인과학회지2009 · 제22권 제1호加味逍遙散의 항산화 효과와 serotonin 대사과정에 미치는 영향심태경, 이상룡, 정인철대한한방신경정신과학회 · 동의신경정신과학회지2011 · 제22권 제1호淸血丹의 6-hydroxydopamine에 의해 유발된 독성에 대한 신경세포보호효과김광호, 김종우, 오명숙, 정선용, 조성훈, 황의완대한한방신경정신과학회 · 동의신경정신과학회지2009 · 제20권 제1호

이 간행물의 다른 논문 (16건)

감송향이 수지상세포 성숙에 미치는 영향정현철, 오광우, 조한백, 김송백, 정지혜, 최창민pp. 14-25四物湯加味方의 항산화 활성에 대한 실험적 연구고은비, 김동일, 정재중, 성정석, 구선영pp. 26-37四物湯加味方의 항암효과에 대한 실험적 연구구선영, 김동일, 정재중, 성정석, 임새롬pp. 38-55補陽還五湯이 실험적으로 유발된 흰쥐의 자궁내막증에 미치는 영향김형준, 임성민, 이동녕, 서일복pp. 56-77威靈仙이 RANKL 처리 RAW 264.7 Cell에 미치는 영향임현정, 유정은, 유동열, 송영훈pp. 78-90
원문 보기