六鬱湯의 산화적 스트레스에 대한 뇌세포 보호효과
Neuroprotective Effect of Yukul-tang against the Oxidative Stress
2
조회
0
다운로드
3
인용
0
참고문헌
키워드
초록
Purpose: In this rapidly aging society, the research and development of traditional oriental medicine treatment is one of the critical factors to protect the increasing neuro-degenerative disorders. In this study, we wanted to verify the effect of Yukul-tang (YUT) on neuro-degenerative disease model by assessing the antioxidant and anti-inflammation effects. Methods: To assess the antioxidant effects of YUT, we carried out DPPH radical and ABTS radical scavenging assays and determined the total polyphenolic contents in YUT. To evaluate the neuro-protective effects of YUT, we performed the MTT and ROS assays and TH immunohistochemistry, NO and TNF-α assays in SH-SY5Y or mesencephalic dopaminergic neurons damaged by 6-OHDA. Results: The treatment of YUT showed eliminating effects on DPPH radical and ABTS radical. it showed deterring effects on ROS, NO and TNF-α and protecting effects on TH-positive cell in SH-SY5Y cells or mesencephalic dopaminergic neurons. Especially in the case of the treatment of YUT with 0.2ug/mL + 6-OHDA 10uM, the protective effect on dopaminergic neurons was most outstanding. Conclusion: In this study, we have demonstrated that YUT has an antioxidant effect and a neuro-protective effect on neuro-degenerative disease model caused by neurotoxin such as 6-OHDA. The results of our present study suggest that YUT can be useful agent to prevent and to treat neuro-degenerative diseases.
Abstract
Purpose: In this rapidly aging society, the research and development of traditional oriental medicine treatment is one of the critical factors to protect the increasing neuro-degenerative disorders. In this study, we wanted to verify the effect of Yukul-tang (YUT) on neuro-degenerative disease model by assessing the antioxidant and anti-inflammation effects. Methods: To assess the antioxidant effects of YUT, we carried out DPPH radical and ABTS radical scavenging assays and determined the total polyphenolic contents in YUT. To evaluate the neuro-protective effects of YUT, we performed the MTT and ROS assays and TH immunohistochemistry, NO and TNF-α assays in SH-SY5Y or mesencephalic dopaminergic neurons damaged by 6-OHDA. Results: The treatment of YUT showed eliminating effects on DPPH radical and ABTS radical. it showed deterring effects on ROS, NO and TNF-α and protecting effects on TH-positive cell in SH-SY5Y cells or mesencephalic dopaminergic neurons. Especially in the case of the treatment of YUT with 0.2ug/mL + 6-OHDA 10uM, the protective effect on dopaminergic neurons was most outstanding. Conclusion: In this study, we have demonstrated that YUT has an antioxidant effect and a neuro-protective effect on neuro-degenerative disease model caused by neurotoxin such as 6-OHDA. The results of our present study suggest that YUT can be useful agent to prevent and to treat neuro-degenerative diseases.
참고문헌 (62)
- "대한민국 통계청"[URL]
- 김동일, "갱년기클리닉의 운영방안과 활용 약물에 관한 고찰", 대한한방부인과학회지, 13(2), pp.418~436 (2000)
- 김진홍, "알츠하이머 질환과 에스트로겐", 대한폐경회지, 6(1), pp.3~11 (2000)
- 정병주, "파킨슨병으로 유발된 진전이 한약 치료를 통하여 호전된 치험 1례", 대한한방내과학회지, 27(4), pp.954~961 (2006), 대한한방내과학회
- 방혜연, "更年期障碍에 關한 文獻的 考察", 동의신경정신과 학회지, 3(2), pp.143~159 (1992)
- Brann DW, "Neurotropic and Neuroprotective Actions of Estrogen: Basic Mechanisms and Clinical Implications", Steroids, 72(5), pp.381~405 (2007)
- Hurn PD, "Estrogen as a neuroprotectant in stroke", J Cereb Blood Flow Metab, 20(4), pp.631~652 (2000)
- Haaxma Ca, "Gender differences in Parkinson's disease", J Neurol Neurosurg Psychiatry, 8, pp.819~824 (2007)
- 강한승, "폐경기모델 백서 해마에서 식물성 에스트로젠에 의한 뇌-혈액장벽 유전자 occludin 발현의 변화", 환경생물, 24(2), pp.166~171 (2006), 한국환경생물학회
- 대한폐경학회 편찬위원회, "폐경기 여성의 관리", pp.62~65 (2001), 군자출판사
- Elisabetta V, "Estrogen anti-inflammatory activity in brain: a therapeutic opportunity for menopause and neurodegenerative disease", Frontiers in neuroendocrinology, 29, pp.507~519 (2008)
- 이정호, "폐경 여성에서 호르몬 요법시 인지 능력과 치매에 대한 효과", 대한폐경회지, 11(2), pp.85~91 (2005)
- Rossouw JE, "Risks and benefits of estrogen plus progestin in healthy postmenopausal women: Principal results from the Women's Health Intiative randomized controlled trial", JAMA, 288(3), pp.321~333 (2002)
- Stephen RR, "Effect of estrogen plus progestin on global cognitive function in postmenopausal women: the women's health initiative memory test: a randomized controlled trial", JAMA, 289(20), pp.2663~2672 (2003)
- 한의부인과학 교재편찬위원회, "한의부인과학", pp.221~222 (2002), 정담
- 許浚, "東醫寶鑑", pp.163~166 (1988), 大星文化社
- 龔信, "古今醫鑑", pp.98~100 (1990), 科學技術出版社
- 裴庚哲, "標準臨床方劑學", pp.300~302 (1995), 傳統醫學硏究所
- 楊蘊祥, "古今名方", pp.168~169 (1983), 科學技術出版社
- 李尙仁, "方劑學", pp.228~229 (1998), 영림사
- 조영도, "六鬱湯이 拘束스트레스 흰쥐의 胃潰瘍 및 血中 Catecholamine 含量에 미치는 영향", 경희한의대논문집, 15, pp.431~448 (1992)
- 조충식, "육울탕이 백서의 갑상선기능항진증에 미치는 영향", 대한본초학회지, 21(4), pp.11~17 (2006), 대한본초학회
- 주미선, "반룡환의 흰쥐태아중뇌에서의 도파민세포 보호효과", 대한본초학회지, 23(3), pp.53~60 (2008), 대한본초학회
- 김효근, "백자인의 6-하이드록시도파민으로 유도된 뇌세포독성에 대한 보호효과", 대한본초학회지, 23(3), pp.19~25 (2008), 대한본초학회
- 김현주, "加味補陽還五湯과 八味合聰明湯의 microglia 보호, 항산화 및 acetylcholinesterase 억제 효과", 동의신경정신과학회지, 19(2), pp.65~76 (2008), 대한한방신경정신과학회
- 이갑득, "노박덩굴에 함유된 celastrol 성분의 파킨슨병을 유발시킨 쥐에서의 도파민 신경세포 보호효과", 대한한의학회지, 29(4), pp.94~103 (2008), 대한한의학회
- 강상렬, "釣鉤藤이 뇌허혈을 유발시킨 백서에서의 뇌신경보호효과", 대한한의학회지, 23(1), pp.1~10 (2002), 대한한의학회
- 김범회, "Gerbil 전뇌허혈에 대한 大黃의 신경보호효과", 대한한의학회지, 23(3), pp.492~502 (2002), 대한한의학회
- 정혁상, "防己가 백서 중대뇌동맥 폐쇄에 의한 국소뇌허혈손상에 미치는 보호효과", 대한한의학회지, 22(1), pp.10~21 (2001)
- Im HI, "Baicalein Prevents 6 - Hydroxydopamine-Induced Dopaminergic Dysfunction and Lipid Peroxidation in Mice", J Pharmacol Sci, 98, pp.185~189 (2005)
- 정성욱, "일시적 국소 뇌허혈 흰쥐모델에서 祛風至寶丹의 뇌손상 및 인지기능 보호효과", 대한한의학회지, 26(2), pp.52~62 (2005), 대한한의학회
- 이민정, "황련해독탕(黃連解毒湯)의 4-VO로 유발한 흰쥐뇌허혈에 대한 신경보호효과", 대한한의학회지, 23(4), pp.811~818 (2002), 대한한의학회
- 조은희, "Neuroprotective Effect of HyulBuChookAu-Tang on Focal Cerebral Ischemia of the Rats", 대한한의학회지, 27(2), pp.70~85 (2006), 대한한의학회
- 강봉주, "MPP+와 6-OHDA에 대한 한약탕제의 보호효과 연구", 한국한의학연구원논문집, 5(1), pp.119~131 (1999)
- 박진성, "생체외 알츠하이머병 실험 모델에서 星香正氣散加蒲公英의 효과에 관한 연구", 동의신경정신과학회지, 12(2), pp.157~171 (2001)
- 민헌기, "임상내분비학", pp.337~345 (1990), 고려의학
- 민성기, "최신정신의학", pp.18~33 (1998), 일조각
- 신문균, "신경해부학", pp.45~46 (1992), 현문사
- Shankar JC, "Dopaminergic neurons", The International Journal of Biochemistry and Cell Biology, 37, pp.942~946 (2005)
- 조광훈, "歸脾湯과 二神交濟丹이 拘束스트레스 생쥐의 腦部位別 Mono-amines 含量에 미치는 영향", 동의경산한의학 학술대회, 통권 : 5, pp.46~61 (2001)
- 류재규, "歸脾湯과 Ascorbic Acid가 熱 및 游泳 Stress Guinea Pig의 뇌 Catecholamine 함량에 미치는 영향", 동의신경정신과 학회지, 6(1), pp.41~49 (1995)
- 송필정, "養心湯 및 養心湯加柿葉이 拘束Stress 흰쥐의 腦部位別 Catecholamine 함량에 미치는 영향", 동의신경정신과 학회지, 8(1), pp.49~68 (1997)
- 김성호, "淸肝逍遙散의 항스트레스 효과에 대한 실험적 연구", 동의신경정신과 학회지, 6(1), pp.61~68 (1995)
- Gonzalez MM, "Light deprivation damages monoamine neurons and produces a depressive behavioral phenotype in rats", PNAS, 105(12), pp.4898~4903 (2008)
- 이정호, "逍遙散과 淸肝逍遙散이 스트레스 생쥐의 腦部位別 Monoamines 含量에 미치는 影響", 동의신경정신과 학회지, 9(2), pp.1~14 (1998)
- 홍성유, "The effect of Bupleurum falcatum L. on the catecholamines of depression rat model", 대한본초학회지, 18(4), pp.245~253 (2003), 대한본초학회
- Berg JA, "Menopausal symptom perception and severity: results from a screening questionnaire", J Clin Nurs, 17(7), pp.940~948 (2008)
- 정선형, "갱년기 장애의 심한 근골격계 통증을 생활사건 스트레스와 연관지어 고찰한 치험 1례", 대한한방부인과학회지, 21(2), pp.252~262 (2008), 대한한방부인과학회
- 陳柱木刁, "金元四大家 醫學全書(下)", pp.328~329 (2007), 法仁文化社
- 李梴, "編註醫學入門", pp.336~337 (1988), 南山堂
- 黃義完, "東醫精神醫學", pp.608~611 (1987), 現代醫學書籍社
- 文濬典, "東醫病理學", pp.60~62 (1990), 高文社
- Nishizawa M, "Non-reductive scavenging of 1,1-Diphenyl-2- picrylhydrazyl (DPPH) by peroxyradical: a useful method for quantitative analysis of peroxyradical", Chem Pharm Bull, 53(6), pp.714~716 (2005)
- Re R, "Antioxidant activity applying an improved ABTS radical cation decolorization assay", Free Radic Biol Med, 26(9), pp.1231~1237 (1999)
- Halliwell B, "Are polyphenols antioxidants or pro-oxidants? What do we learn from cell culture and in vivo studies?", Arc Biochem and Biophysics, 476, pp.107~112 (2008)
- Sauer H, "Progressive degeneration of nigrostriatal dopamine neurons following intrastriatal terminal lesions with 6-hydroxydopamine: A combined retrograde tracing and immunocytochemical study in the rat", Neuroscience, 59(2), pp.401~415
- Wu Y, "unlike MPP+, apoptosis induced by 6-OHDA in PC12 cells is independent of mitochondrial inhibition", Neuroscience Letters, 221, pp.69~71 (1996)
- Singh S, "Neuroprotection of the nigrostriatal dopaminergic neurons by melatonin in hemiparkinsonium rat", Indian J Med Res, 124, pp.419~426 (2006)
- Hanrott K, "6-Hydroxydopamine -induced Apoptosis is mediated via Extracellular auto-Oxidation and Caspase 3-dependent Activation of Protein Kinase Cdelta", The Journal Of Biological Chemistry, 281(9), pp.5373~5382 (2006)
- Meredith GE, "Animal Models of Parkinson's Disease Progression", Acta Neuropathol, 115(4), pp.385~398 (2008)
- Mosley RL, "Neuroinflammation, Oxidative Stress and the Pathogenesis of Parkinson's Disease", Clin Neurosci Res, 6(5), pp.261~281 (2006)
- Whitton PS, "Inflammation as a causative factor in the aetiology of Parkinson's disease", British Journal Pharmacology, 150, pp.963~976 (2007)
