본문 바로가기
KERD
논문KCI 등재

歸脾湯과 歸脾湯加味方의 항산화 효과 및 6-Hydroxydopamine에 대한 PC12 세포 보호효과 비교연구

The Antioxidative and Neuroprotective Effect of Guibi-tang(Guipitang) and Guibi-tang gamibang(Guipitang jiaweifang) on PC12 cells.

조성훈|김종우|황의완|오명숙|정선용|임재환
대한한방신경정신과학회 (The Korean Society Of Oriental Neuropsychiatry)|2009.03|동의신경정신과학회지 (The Korean Society of Oriental Neuropsychiatry)|제20권 제1호|pp. 1-20

2

조회

0

다운로드

4

인용

0

참고문헌

키워드

AntioxidativeNeuroprotectiveGuibi-tang(Guipitang)Guibi-tang gamibang(Guipitang jiaweifang)
주제분류한의학

초록

Objectives : This Study was performed to assess the antioxidative and neuroprotective effect of Guibi-tang(Guipi-tang) and Guibi-tang gamibang(Guipitang jiaweifang) on PC12 cells. Methods : The antioxidative effect was investigated through the DPPH radical and ABTS cation scavenging methods and total polyphenol amount of Guibi-tang(Guipitang) and Guibi-tang gamibang(Guipitang jiaweifang). The neuroprotective effect of Guibi-tang(Guipitang) and Guibi-tang gamibang(Guipitang jiaweifang) was assessed using MTT assay in PC12 cells. The scavenging effect of Guibi-tang(Guipitang) and Guibi-tang gamibang(Guipitang jiaweifang) on NO and ROS production induced by 6-OHDA in PC12 cells was evaluated, as well as the attenuating effect of Guibi-tang gamibang(Guipitang jiaweifang) on GSH reduction. Results : 1. Guibi-tang(Guipitang) and Guibi-tang gamibang(Guipitang jiaweifang) had concentration-dependent scavenging activities of DPPH radical. 2. Guibi-tang(Guipitang) and Guibi-tang gamibang(Guipitang jiaweifang) had concentration-dependent scavenging activities of ABTS cation. 3. Total polyphenol amount of Guibi-tang(Guipitang) and Guibi-tang gamibang(Guipitang jiaweifang) was calculated 79.10±2.20 ㎍/10 ㎎ and 121.03±1.11 ㎍/10 ㎎, respectively. 4. Cell viability of Guibi-tang(Guipitang) was increased in a dose dependent manner. Guibi-tang gamibang(Guipitang jiaweifang) was increased at low concentrations, but decreased at high concentrations. 5. In Guibi-tang(Guipitang), cell viability of PC12 cell treated with 6-OHDA was decreased by pre-treatment, and increased by post- and co- treatment. Cell viability of Guibi-tang gamibang(Guipitang jiaweifang) showed variable effects by pre-treatment, but increased by post- and co- treatment. 6. NO production rate of Guibi-tang(Guipitang) didn't show a significant effect, but that of Guibi-tang gamibang(Guipitang jiaweifang) was decreased in a dose dependent manner. 7. ROS production rate of Guibi-tang(Guipitang) was decreased at some concentrations. In Guibi-tang gamibang(Guipitang jiaweifang), ROS production rate was decreased at high concentrations. 8. Guibi-tang gamibang(Guipitang jiaweifang) protected the 6-OHDA-induced GSH reduction. Conclusions : These results demonstrate that both Guibi-tang(Guipitang) and GBTGMB have antioxidative and neuroprotective effect, but Guibi-tang gamibang(Guipitang jiaweifang) has more antioxidative and neuroprotective effect than Guibi-tang.

Abstract

Objectives : This Study was performed to assess the antioxidative and neuroprotective effect of Guibi-tang(Guipi-tang) and Guibi-tang gamibang(Guipitang jiaweifang) on PC12 cells. Methods : The antioxidative effect was investigated through the DPPH radical and ABTS cation scavenging methods and total polyphenol amount of Guibi-tang(Guipitang) and Guibi-tang gamibang(Guipitang jiaweifang). The neuroprotective effect of Guibi-tang(Guipitang) and Guibi-tang gamibang(Guipitang jiaweifang) was assessed using MTT assay in PC12 cells. The scavenging effect of Guibi-tang(Guipitang) and Guibi-tang gamibang(Guipitang jiaweifang) on NO and ROS production induced by 6-OHDA in PC12 cells was evaluated, as well as the attenuating effect of Guibi-tang gamibang(Guipitang jiaweifang) on GSH reduction. Results : 1. Guibi-tang(Guipitang) and Guibi-tang gamibang(Guipitang jiaweifang) had concentration-dependent scavenging activities of DPPH radical. 2. Guibi-tang(Guipitang) and Guibi-tang gamibang(Guipitang jiaweifang) had concentration-dependent scavenging activities of ABTS cation. 3. Total polyphenol amount of Guibi-tang(Guipitang) and Guibi-tang gamibang(Guipitang jiaweifang) was calculated 79.10±2.20 ㎍/10 ㎎ and 121.03±1.11 ㎍/10 ㎎, respectively. 4. Cell viability of Guibi-tang(Guipitang) was increased in a dose dependent manner. Guibi-tang gamibang(Guipitang jiaweifang) was increased at low concentrations, but decreased at high concentrations. 5. In Guibi-tang(Guipitang), cell viability of PC12 cell treated with 6-OHDA was decreased by pre-treatment, and increased by post- and co- treatment. Cell viability of Guibi-tang gamibang(Guipitang jiaweifang) showed variable effects by pre-treatment, but increased by post- and co- treatment. 6. NO production rate of Guibi-tang(Guipitang) didn't show a significant effect, but that of Guibi-tang gamibang(Guipitang jiaweifang) was decreased in a dose dependent manner. 7. ROS production rate of Guibi-tang(Guipitang) was decreased at some concentrations. In Guibi-tang gamibang(Guipitang jiaweifang), ROS production rate was decreased at high concentrations. 8. Guibi-tang gamibang(Guipitang jiaweifang) protected the 6-OHDA-induced GSH reduction. Conclusions : These results demonstrate that both Guibi-tang(Guipitang) and GBTGMB have antioxidative and neuroprotective effect, but Guibi-tang gamibang(Guipitang jiaweifang) has more antioxidative and neuroprotective effect than Guibi-tang.

참고문헌 (65)

  1. 최병기, "활성산소와 질환", pp.3~ (2004), 신일상사
  2. Stadtman ER, "Protein oxidation and aging", Science, 257(5074), pp.120~124 (1992)
  3. Gutteridge JM, "Comments on review of free radicals in biology and medicine", Free Radicals in biology and medicine, 12(1), pp.93~95 (1992)
  4. Halliwell B, "Oxidative stress and disease. Free radicals in biology and medicine", pp.617~783 (2000), Oxford University Press
  5. Yagi, K, "Lipid peroxides and human disease", Chem. Phys. Lipids, 45, pp.337~ (1987)
  6. Kikuchi S, "Glutamate neurotoxicity in mesencephalic dopaminergic neurons in culture", J Neuosci Res, 36, pp.558~569 (1993)
  7. Adelson R, "Oxidative damage to DNA: Relation to species metabolic and life span", Natl Acad Sci USA, 85, pp.2706~2708 (1998)
  8. Bendich A, "DDM : The antioxidant role of vitamin C", Free Radical Biol Med, 2, pp.419~444 (1986)
  9. Christian Bhel, "Alzheimer's disease and Oxidative stress", Progress in Neurobiology, 57, pp.301~323 (1999)
  10. Eckert A, "Mitochondrial dysfunction , apoptotic cell death, and Alzheimer's disease", Biochem Parm, 66(8), pp.1627~1634 (2003)
  11. 양준영, "6-OHDA에 의해 유도된 파킨슨질환 동물 모델에서 GAPDH 단백질의 변화에 대한 연구", pp.2004
  12. 嚴用和, "濟生方", pp.117~ (1980), 人民衛生出版社
  13. 薛己, "內科摘要", pp.41~42 (1985), 江蘇科學技術出版社
  14. 許浚, "東醫寶鑑", pp.47~ (1966), 南山堂
  15. 강순수, "바른방제학", pp.190~191 (1996), 대성문화사
  16. 동의과학원, "동의처방대전", pp.114~ (1993), 여강출판사(1)
  17. 윤용갑, "東醫方劑와 處方解說", pp.631~635 (2002), 의성당
  18. 윤길영, "동의임상방제학", pp.516~ (1992), 명보출판사
  19. 박선동, "歸脾湯및 그 構成 藥物群이 抗酸化 效果에 미치는 영향", 대한본초학회지, 16(1), pp.11~27 (2001)
  20. 박찬원, "脾機能이 學習과 記憶에 미치는 影響에 대한 實驗的 연구", 대한한의학회지, 20(4), pp.39~49 (2000)
  21. 강익현, "歸脾湯이 Glutamate에 의한 C6 glial Cell의 Apoptosis에 미치는 영향", pp.2001
  22. 전희준, "歸脾湯이 Glutamate에 의한 성상세포의 손상에 미치는 영향", pp.2002
  23. 오명숙, "歸脾湯이 흰쥐의 기억력 향상과 해마부위의 세포증식에 미치는 영향에 대한 연구", 醫林, 320, pp.46~49 (2005)
  24. Seo KI, "Antimicrobial and antioxidatice activities of Corni Fructus extracts", Korean J Postharvest Sci Technol, 6, pp.99~103 (1999)
  25. 최선영, "오미자의 이화학적 특성 및 항산화 활성", 한국식품영양학회지, 21(1), pp.35~42 (2008), 한국식품영양학회
  26. Lee JS, "A study on the general components and minerals in parts of Omija(Schizandra chinensis Baillon)", Kor J Dietry culture, 4, pp.173~176 (1989)
  27. Blois MS, "Antioxidant determination by the use of a stable free radical", Nature, 181, pp.1190~1200 (1958)
  28. George P, "Antioxidant Effect of Natural Phenols on Olive Oil", Journal of the American Oil Chemists' Society, 68, pp.669~671 (1991)
  29. Branen AL, "Toxicological and biochemistry of butylated hydroxytoluene, butylated hydroxyanisole", J. Am. Oil. Chem. Soc, 52, pp.59~63 (1975)
  30. Hatano T, "Constituents of natural medicines with scavenging effects on active oxygen species-tannins and related polyphenols", Natural Medicines, 49, pp.357~ (1995)
  31. Masaki H, "Active-oxygen scavenging activity of plant extracts", Biol. Pharm. Bull, 18, pp.162~ (1995)
  32. Beckman KB, "The free radical theory of aging matures", Physiol Rev, 78, pp.547~581 (1998)
  33. Chung HY, "The inflammation hypothesis of aging : molecular modulation by calorie restriction", Ann NY Acad Sci, 928, pp.327~335 (2001)
  34. Vincenzo DM and Ennio E, "Biochemical and Therapeutic effects of antioxidants in the treatment of Alzheimer's disease, Parkinson's disease, and Amyotrophic lateral sclerosis", CNS & Neurological disorders, 2, pp.95~107 (2003)
  35. 한백수, "6-hydroxydopamine과 1-methyl-4-phenylpyridinium에 의해 유도된 도파민성 신경세포 사멸에 대한 특성 연구", pp.2003
  36. Cohen G, "Oxy-radical toxicity in catecholamine neurons", Neurotoxycology, 5, pp.77~82 (1984)
  37. Cohen G, "Free radicals, oxidative stress, and neurodegeneration. in: Neurodegenerative Disease" (1994), W.B
  38. 홍원식, "校編 精校黃帝內經素問", pp.23~25 (1985), 동양의학연구원출판부
  39. 박상민, "파킨슨병의 한의학적 고찰 -병인병리와 침구요법을 중심으로-", 대한침구학회지, 21(1), pp.202~210 (2004), 대한침구학회
  40. 張從正, "儒門事親", pp.1~2 (1967), 旋風出版社
  41. 한의과대학 본초학교수, "본초학", pp.137~ (1992), 도서출판영림사
  42. 嚴用和, "重訂嚴氏濟生方", pp.115~117 (1980), 인민위생출판사
  43. 한의과대학 방제학교수, "방제학", pp.290~292 (1999), 도서출판영림사
  44. Park YK, "The antidiabetic effects of from Cornus officinalis seed", Chung-Ang J Pharm Sci, 9, pp.13~22 (1995)
  45. Chung SR, "Toxicity and lectins constituents from the seed of Cornus officinalis", Korean J Pharmacogn, 24, pp.177~182 (1993)
  46. 전연희, "산수유(Cornus officianalis) 에탄올 추출물의 항산화, 항돌연변이 활성 및 암세포 성장 억제 효과", 한국식품영양과학회지, 37(1), pp.1~7 (2008), 한국식품영양과학회
  47. Dai Y, "Inhibition of Corni fructus on experimental inflammation", Chung Kuo Chung Yao Ts Chih, 17, pp.307~309 (1992)
  48. Yamahara J, "Antidiabetic principles of Corni Fructus experimental diabetes induced by streptozotocin", Yakugaku Zasshi, 101, pp.86~90 (1981)
  49. Lee JS, "Effect of water extracts in Omija(Schizandra chinensis Baillon) on alcohol metabolism", Kor J Dietry culture, 5, pp.259~263 (1990)
  50. Brand-Williams W, "Use of free radical method to evaluate antioxidant activity", Lebensm. Wiss. Technol, 28, pp.25~30 (1995)
  51. Re R, "Antioxidant activity applying improved ABTS radical cation decolorization assay", Free Radical Bio. Med, 26, pp.1231~1237 (1999)
  52. 김진율, "제조 공정에 따른 커피 항산화능의 변화와 커피 박 추출물의 식품 소재화", pp.2008
  53. Kochi K, "Synthesis of Novel Polyphenols Consisted of Ferulic and Gallic Acids", Bioorganic & Medicinal Chemistry, 10, pp.1069~1075 (2002)
  54. Zhentian L, "Use of Methanolysis for the Determination of Total Ellagic and Gallic Acid Contents of Wood and Foods Products", J. Agric. Food Chem, 49, pp.1165~1168 (2001)
  55. Sladowski D, "An improved MTT assay", J Immunol Methods, 157, pp.203~207 (1993)
  56. Bernas T, "Mitochondrial and nonmitochondrial reduction of MTT : Interaction of MTT with TMRE, JC-1, and NAO Mitochondrial fluorescent probes", Cytometry, 47, pp.236~242 (2002)
  57. Halliwell B, "Free Radicals in Biology and Medicine(3rd ed.)" (1999), Oxford University Press
  58. Beckman JS, "Apparent hydroxyl radical production by peroxynitrite : Implications for endothelial injury from nitric oxide and superoxide", Proc. Natl. Acad. Sci. USA., 통권 : 87, pp.1620~1624 (1990)
  59. Stamler JS, "Oxidative modifications in nitrosative stress", Nat. Stract Biol, 5, pp.247~249 (1998)
  60. Ashai M, "Inactivation of glutathione peroxidase by nitric oxide. Implication for cytotoxity", J. Biol. Chem, 270, pp.21335~21339 (1995)
  61. Becker K, "Inhibition of human glutathione reductase by S-nitrosoglutathione", Eur. J. Biochem, 234, pp.472~478 (1995)
  62. Park SN, "Skin aging and antioxidants", J. Korean Soc. Cosmetic Chem, 23, pp.75~132 (1997)
  63. Antebi H, "Oxygen Tadical in Chemistry and Biology", pp.757~760 (1984)
  64. Sies H, "Glutathione disulfide(GSSG) efflux from cells and tissues", Methods Enzymol, 105, pp.445~451 (1984)
  65. Orrenius S, "Mechanism of oxidative cell damage", pp.47~63 (1993), Birkhauser Verlag, Basel

관련 논문AI추천

加味逍遙散의 抗酸化 效果 및 神經細胞 保護效果이경섭, 이성한, 이진무, 이창훈, 장준복, 조정훈대한한방부인과학회 · 대한한방부인과학회지2010 · 제23권 제3호六鬱湯의 산화적 스트레스에 대한 뇌세포 보호효과이경섭, 이진무, 이창훈, 장준복, 정선형, 조정훈대한한방부인과학회 · 대한한방부인과학회지2009 · 제22권 제1호淸血丹의 6-hydroxydopamine에 의해 유발된 독성에 대한 신경세포보호효과김광호, 김종우, 오명숙, 정선용, 조성훈, 황의완대한한방신경정신과학회 · 동의신경정신과학회지2009 · 제20권 제1호人蔘養營湯의 산화적 stress에 대한 뇌세포 보호효과김승현, 이경섭, 이진무, 이창훈, 장준복, 조정훈대한한방부인과학회 · 대한한방부인과학회지2009 · 제22권 제1호加味歸脾湯이 P815 세포의 serotonin 대사 과정에 미치는 영향노동진, 정인철대한한방신경정신과학회 · 동의신경정신과학회지2010 · 제21권 제1호分心氣飮의 도파민 세포 보호 효과김로사, 이경섭, 이진무, 이창훈, 장준복, 조정훈대한한방부인과학회 · 대한한방부인과학회지2009 · 제22권 제2호

이 간행물의 다른 논문 (13건)

淸血丹의 6-hydroxydopamine에 의해 유발된 독성에 대한 신경세포보호효과김광호, 조성훈, 김종우, 황의완, 오명숙, 정선용pp. 21-42還少丹이 microglia 염증반응 cytokine과 건망증 생쥐모델에 미치는 영향이상룡, 윤종천, 정인철pp. 43-58溫膽湯合聰明湯 열수추출물과 초미세분말이 cytokine과 건망증 생쥐모델 기억력감퇴에 미치는 영향이상룡, 정인철, 장영주pp. 59-74Neuroprotective Effect of Yukmijihwang-tang(Liuweidihuangtang) Gamibang on the Deficits of Learning and Memory in Trimethyltin-Intoxicated Rats김근우, 구병수, 정희상pp. 75-88『方藥合編』에 수재된 神經精神科 관련 주요 處方에 대한 硏究김지훈, 구병수, 김근우pp. 89-106
원문 보기