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Mirodenafil Prevents Bladder Dysfunction Induced by Chronic Bladder Ischemia in Rats

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Purpose: To investigate the protective effect of mirodenafil on bladder function in a rat model of chronic bladder ischemia(CBI). Methods: Twenty-four Sprague-Dawley rats were randomized to three groups: untreated, sham-operated rats (control group);untreated, CBI model rats (CBI group); and CBI rats treated daily with 4 mg/kg mirodenafil (CBI+mirodenafil group). TheCBI and CBI+mirodenafil groups underwent endothelial injury to the iliac arteries and were fed a 2% cholesterol diet afterinjury. Four weeks after surgery, the CBI+mirodenafil group started daily treatment with mirodenafil for four weeks. Eightweeks after surgery, continuous in vivo cystometry and in vitro organ bath studies of detrusor muscle strips were performed. Results: In vivo cystometry revealed that the rats in the CBI group had a significantly higher micturition frequency, lowerbladder capacity, and lower compliance than the rats in the control and CBI+mirodenafil groups. The detrusor muscle stripstudy showed that the magnitude of the carbachol-induced contractile response was significantly lower in the CBI group comparedto either the control or CBI+mirodenafil group. Addition of daily mirodenafil after induction of CBI decreased thecontractile response, compared to untreated CBI rats. CBI induced submucosal fibrosis and degenerative changes in bladderwalls, which was reversed by the addition of mirodenafil. Conclusions: Daily treatment with mirodenafil showed protective effects against bladder dysfunction resulting from CBI inrats.

Abstract

Purpose: To investigate the protective effect of mirodenafil on bladder function in a rat model of chronic bladder ischemia(CBI). Methods: Twenty-four Sprague-Dawley rats were randomized to three groups: untreated, sham-operated rats (control group);untreated, CBI model rats (CBI group); and CBI rats treated daily with 4 mg/kg mirodenafil (CBI+mirodenafil group). TheCBI and CBI+mirodenafil groups underwent endothelial injury to the iliac arteries and were fed a 2% cholesterol diet afterinjury. Four weeks after surgery, the CBI+mirodenafil group started daily treatment with mirodenafil for four weeks. Eightweeks after surgery, continuous in vivo cystometry and in vitro organ bath studies of detrusor muscle strips were performed. Results: In vivo cystometry revealed that the rats in the CBI group had a significantly higher micturition frequency, lowerbladder capacity, and lower compliance than the rats in the control and CBI+mirodenafil groups. The detrusor muscle stripstudy showed that the magnitude of the carbachol-induced contractile response was significantly lower in the CBI group comparedto either the control or CBI+mirodenafil group. Addition of daily mirodenafil after induction of CBI decreased thecontractile response, compared to untreated CBI rats. CBI induced submucosal fibrosis and degenerative changes in bladderwalls, which was reversed by the addition of mirodenafil. Conclusions: Daily treatment with mirodenafil showed protective effects against bladder dysfunction resulting from CBI inrats.

참고문헌 (35)

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