치과용 유니트 수관에서 분리한 세균의 부착 및 바이오필름 형성 능력
Adhesion and Biofilm Formation Abilities of Bacteria Isolated from Dental Unit Waterlines
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The purpose of our study is to compare the adhesion and biofilm formation abilities of isolates from water discharged from dental unit waterlines (DUWLs). Bacteria were isolated from a total of 15 DUWLs. Twelve isolates were selected for the experiment. To confirm the adhesion ability of the isolates, each isolate was attached to a glass coverslip using a 12-well plate. Plates were incubated at 26oC for 7 days, and the degree of adhesion of each isolate was scored. To verify the biofilm formation ability of each isolate, biofilms were allowed to form on a 96-well polystyrene flat-bottom microtiter plate. The biofilm accumulations of all isolates formed at 26oC for 7 days were identified and compared. A total of 56 strains were isolated from 15 water samples including 12 genera and 31 species. Of the 56 isolates, 12 isolates were selected according to the genus and used in the experiment. Sphingomonas echinoides, Methylobacterium aquaticum , and Cupriavidus pauculus had the highest adhesion ability scores of +3 among 12 isolates. Among these three isolates, the biofilm accumulation of C. pauculus was the highest and that of S. echinoides was the third-most abundant. The lowest biofilm accumulations were identified in Microbacterium testaceum and M. aquaticum. Most isolates with high adhesion ability also exhibited high biofilm formation ability. Analysis of adhesion and biofilm formation of the isolates from DUWLs can provide useful information to understand the mechanism of DUWL biofilm formation and development.
Abstract
The purpose of our study is to compare the adhesion and biofilm formation abilities of isolates from water discharged from dental unit waterlines (DUWLs). Bacteria were isolated from a total of 15 DUWLs. Twelve isolates were selected for the experiment. To confirm the adhesion ability of the isolates, each isolate was attached to a glass coverslip using a 12-well plate. Plates were incubated at 26oC for 7 days, and the degree of adhesion of each isolate was scored. To verify the biofilm formation ability of each isolate, biofilms were allowed to form on a 96-well polystyrene flat-bottom microtiter plate. The biofilm accumulations of all isolates formed at 26oC for 7 days were identified and compared. A total of 56 strains were isolated from 15 water samples including 12 genera and 31 species. Of the 56 isolates, 12 isolates were selected according to the genus and used in the experiment. Sphingomonas echinoides, Methylobacterium aquaticum , and Cupriavidus pauculus had the highest adhesion ability scores of +3 among 12 isolates. Among these three isolates, the biofilm accumulation of C. pauculus was the highest and that of S. echinoides was the third-most abundant. The lowest biofilm accumulations were identified in Microbacterium testaceum and M. aquaticum. Most isolates with high adhesion ability also exhibited high biofilm formation ability. Analysis of adhesion and biofilm formation of the isolates from DUWLs can provide useful information to understand the mechanism of DUWL biofilm formation and development.
참고문헌 (30)
- Walker JT, "Microbiological evaluation of dental unit water systems in general dental practice in Europe", Eur J Oral Sci, 112, pp.412~418 (2004)[DOI]
- O'Donnell MJ, "Management of dental unit waterline biofilms in the 21st century", Future Microbiol, 6, pp.1209~1226 (2011)[DOI]
- Barbeau J, "Multiparametric analysis of waterline contamination in dental units", Appl Environ Microbiol, 62, pp.3954~3959 (1996)
- Singh R, "Microbial diversity of biofilms in dental unit water systems", Appl Environ Microbiol, 69, pp.3412~3420 (2003)[DOI]
- Szymanska J, "Microbial contamination of dental unit waterlines", Ann Agric Environ Med, 15, pp.173~179 (2008)
- Dutil S, "Detection of Legionella spp. by fluorescent in situ hybridization in dental unit waterlines", J Appl Microbiol, 100, pp.955~963 (2006)[DOI]
- Szymanska J, "Opportunistic bacteria in dental unit waterlines: assessment and characteristics", Future Microbiol, 8, pp.681~689 (2013)[DOI]
- Costa D, "Pyrosequencing analysis of bacterial diversity in dental unit waterlines", Water Res, 81, pp.223~231 (2015)[DOI]
- Cobb CM, "How does time-dependent dental unit waterline flushing affect planktonic bacteria levels", J Dent Educ, 66, pp.549~555 (2002)
- Walker JT, "Microbiological evaluation of a range of disinfectant products to control mixed-species biofilm contamination in a laboratory model of a dental unit water system", Appl Environ Microbiol, 69, pp.3327~3332 (2003)[DOI]
- Whitehouse RL, "Influence of biofilms on microbial contamination in dental unit water", J Dent, 19, pp.290~295 (1991)[DOI]
- Meiller TF, "Laboratory evaluation of anti-biofilm agents for use in dental unit waterlines", J Clin Dent, 12, pp.97~103 (2001)
- Meiller TF, "Dental unit waterlines: biofilms, disinfection and recurrence", J Am Dent Assoc, 130, pp.65~72 (1999)[DOI]
- Walker JT, "Microbial biofilm formation in DUWS and their control using disinfectants", J Dent, 35, pp.721~730 (2007)[DOI]
- Liu Y, "Role of bacterial adhesion in the microbial ecology of biofilms in cooling tower systems", Biofouling, 25, pp.241~253 (2009)[DOI]
- Simoes LC, "Influence of the diversity of bacterial isolates from drinking water on resistance of biofilms to disinfection", Appl Environ Microbiol, 76, pp.6673~6679 (2010)[DOI]
- 박종화, "A periodontitis-associated multispecies model of an oral biofilm", Journal of Periodontal & Implant Science, 44(2), pp.79~84 (2014), 대한치주과학회
- Eginton PJ, "Changes in the strength of attachment of micro-organisms to surfaces following treatment with disinfectants and cleansing agents", Lett Appl Microbiol, 27, pp.101~105 (1998)[DOI]
- Modesto A, "Multiple exposures to chlorhexidine and xylitol: adhesion and biofilm formation by Streptococcus mutans", Curr Microbiol, 52, pp.418~423 (2006)[DOI]
- Bodenmiller D, "Development of surface adhesion in Caulobacter crescentus", J Bacteriol, 186, pp.1438~1447 (2004)[DOI]
- Antunes AL, "High vancomycin resistance among biofilms produced by Staphylococcus species isolated from central venous catheters", Mem Inst Oswaldo Cruz, 106, pp.51~55 (2011)[DOI]
- 윤혜영, "Well-Plate를 사용한 치과용 유니트 수관 바이오필름 모델 확립", 치위생과학회지, 17(4), pp.283~289 (2017), 한국치위생과학회[DOI]
- Walker C, "An in vitro biofilm model of subgingival plaque", Oral Microbiol Immunol, 22, pp.152~161 (2007)[DOI]
- de Lillo A, "Novel subgingival bacterial phylotypes detected using multiple universal polymerase chain reaction primer sets", Oral Microbiol Immunol, 21, pp.61~68 (2006)[DOI]
- Stepanovic S, "Quantification of biofilm in microtiter plates: overview of testing conditions and practical recommendations for assessment of biofilm production by staphylococci", APMIS, 115, pp.891~899 (2007)[DOI]
- Yabune T, "Assessment of inhibitory effects of fluoride-coated tubes on biofilm formation by using the in vitro dental unit waterline biofilm model", Appl Environ Microbiol, 74, pp.5958~5964 (2008)[DOI]
- Simoes LC, "Adhesion and biofilm formation on polystyrene by drinking water-isolated bacteria", Antonie Van Leeuwenhoek, 98, pp.317~329 (2010)[DOI]
- Azeredo J, "The role of exopolymers produced by Sphingomonas paucimobilis in biofilm formation and composition", Biofouling, 16, pp.17~27 (2000)[DOI]
- Rickard AH, "Coaggregation between freshwater bacteria within biofilm and planktonic communities", FEMS Microbiol Lett, 220, pp.133~140 (2003)[DOI]
- Yabune T, "Inhibitory effect of PVDF tubes on biofilm formation in dental unit waterlines", Dent Mater, 21, pp.780~786 (2005)[DOI]