치과용 유니트 수관으로부터 분리된 세균의 바이오필름 형성 특징
Biofilm-forming properties of bacteria isolated from dental unit waterlines
1
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
Objectives: The aim of this study was to identify and compare the properties associated with the biofilm-forming abilities of bacteria isolated from water in dental unit waterlines (DUWLs). Methods: Twelve bacterial strains isolated from the output water of DUWLs were cultured in 12-well plates containing polyurethane tubing and R2A medium. The amount of biofilm formed by each strain was evaluated. Based on the amount of biofilm formed, three strains were selected, and the level of adhesion was determined for each strain depending on the inoculation concentration and incubation time. In addition, adhesion inhibitors were identified in the three strains, and their hydrophobicity and extracellular polymeric substances (EPS) formation were compared. Results: Among the 12 strains, the highest levels of biofilm accumulation were seen in Cupriavidus pauculus HY12. C. pauculus HY12 showed higher levels of adhesion at lower bacterial concentrations and shorter incubation times than other strains. The adhesion of C. pauculus HY12 was inhibited by trypsin. Among the three isolates, hydrophobicity and EPS production were the highest in Acidovorax delafieldii HY1. Conclusions: The results of this study provide a basis for understanding the mechanism of DUWL biofilm formation and the development of methods to inhibit biofilm formation.
Abstract
Objectives: The aim of this study was to identify and compare the properties associated with the biofilm-forming abilities of bacteria isolated from water in dental unit waterlines (DUWLs). Methods: Twelve bacterial strains isolated from the output water of DUWLs were cultured in 12-well plates containing polyurethane tubing and R2A medium. The amount of biofilm formed by each strain was evaluated. Based on the amount of biofilm formed, three strains were selected, and the level of adhesion was determined for each strain depending on the inoculation concentration and incubation time. In addition, adhesion inhibitors were identified in the three strains, and their hydrophobicity and extracellular polymeric substances (EPS) formation were compared. Results: Among the 12 strains, the highest levels of biofilm accumulation were seen in Cupriavidus pauculus HY12. C. pauculus HY12 showed higher levels of adhesion at lower bacterial concentrations and shorter incubation times than other strains. The adhesion of C. pauculus HY12 was inhibited by trypsin. Among the three isolates, hydrophobicity and EPS production were the highest in Acidovorax delafieldii HY1. Conclusions: The results of this study provide a basis for understanding the mechanism of DUWL biofilm formation and the development of methods to inhibit biofilm formation.