본문 바로가기
KERD
논문KCI 등재

Bacterial Coaggregation in Bacteria Isolated from Dental Unit Waterlines

0

조회

0

다운로드

0

인용

0

참고문헌

키워드

주제분류치의학

초록

Background: To understand the process of biofilm formation in dental unit waterlines (DUWLs), it is necessary to assess the of bacteria that constitute DUWL biofilms. This study assessed the coaggregation of bacterial species isolated from DUWL biofilms and other related mechanisms. Methods: Coaggregation was macroscopically evaluated. Cultures of the DUWLs isolates were adjusted to the same concentration (optical density [OD]600=1.5). The isolates were mixed in pairs and incubated for 1 hour at room temperature. Subsequently, the coaggregation values were scored. Some coaggregation pairs were observed through fluorescence microscope after staining with SYTOⓇ 9 and propidium iodide. To assess their coaggregation properties, inhibition of coaggregation was performed by adding sugars and amino acids to the coaggregation pairs and the coaggregation values were scored again post-addition. Results: Coaggregation was observed in 360 out of 406 coaggregation pairs, whose coaggregation scores of ranged from one to three points. Five pairs (1.4%) had a coaggregation score of 3 points. Among these five pairs, mannose most frequently inhibited coaggregation. Among the isolates, Cupriavidus pauculus HY12 and Beijerinckia derxii subsp. venezuelae HY7 had the highest number of coaggregation pairs, with scores of 2 and 3. Conclusion: Cupriavidus pauculus HY12 and B. derxii subsp. venezuelae HY7 form many coaggregation pairs and may play important roles in biofilm maturation. The results of this study can be used to propose methods to inhibit the formation of DUWL biofilms by inhibiting coaggregation.

Abstract

Background: To understand the process of biofilm formation in dental unit waterlines (DUWLs), it is necessary to assess the of bacteria that constitute DUWL biofilms. This study assessed the coaggregation of bacterial species isolated from DUWL biofilms and other related mechanisms. Methods: Coaggregation was macroscopically evaluated. Cultures of the DUWLs isolates were adjusted to the same concentration (optical density [OD]600=1.5). The isolates were mixed in pairs and incubated for 1 hour at room temperature. Subsequently, the coaggregation values were scored. Some coaggregation pairs were observed through fluorescence microscope after staining with SYTOⓇ 9 and propidium iodide. To assess their coaggregation properties, inhibition of coaggregation was performed by adding sugars and amino acids to the coaggregation pairs and the coaggregation values were scored again post-addition. Results: Coaggregation was observed in 360 out of 406 coaggregation pairs, whose coaggregation scores of ranged from one to three points. Five pairs (1.4%) had a coaggregation score of 3 points. Among these five pairs, mannose most frequently inhibited coaggregation. Among the isolates, Cupriavidus pauculus HY12 and Beijerinckia derxii subsp. venezuelae HY7 had the highest number of coaggregation pairs, with scores of 2 and 3. Conclusion: Cupriavidus pauculus HY12 and B. derxii subsp. venezuelae HY7 form many coaggregation pairs and may play important roles in biofilm maturation. The results of this study can be used to propose methods to inhibit the formation of DUWL biofilms by inhibiting coaggregation.

참고문헌 (46)

  1. Whitehouse RL, "Influence of biofilms on microbial contamination in dental unit water", J Dent, 19, pp.290~295 (1991)[DOI]
  2. Karpay RI, "Comparison of methods to enumerate bacteria in dental unit water lines", Curr Microbiol, 38, pp.132~134 (1999)[DOI]
  3. Walker JT, "Microbial biofilm formation and contamination of dental-unit water systems in general dental practice", Appl Environ Microbiol, 66, pp.3363~3367 (2000)[DOI]
  4. Yoon H, "Bacterial contamination of dental unit water systems in a student clinical simulation laboratory of college of dentistry", J Dent Hyg Sci, 15, pp.232~237 (2015)[DOI]
  5. Szymańska J, "Evaluation of mycological contamination of dental unit waterlines", Ann Agric Environ Med, 12, pp.153~155 (2005)
  6. Lisboa GM, "Microbial diversity in dental unit waterlines", Acta Odontol Latinoam, 27, pp.110~114 (2014)[DOI]
  7. Costa D, "Pyrosequencing analysis of bacterial diversity in dental unit waterlines", Water Res, 81, pp.223~231 (2015)[DOI]
  8. Yoon HY, "An assessment of the bacterial diversity found in dental unit waterlines using the Illumina MiSeq", Biocontrol Sci, 24, pp.201~212 (2019)[DOI]
  9. Martin MV, "The significance of the bacterial contamination of dental unit water systems", Br Dent J, 163, pp.152~154 (1987)[DOI]
  10. Atlas RM, "Legionella contamination of dental-unit waters", Appl Environ Microbiol, 61, pp.1208~1213 (1995)[DOI]
  11. Szymańska J, "Opportunistic bacteria in dental unit waterlines : assessment and characteristics", Future Microbiol, 8, pp.681~689 (2013)[DOI]
  12. Walker JT, "Control of Legionella pneumophila in a hospital water system by chlorine dioxide", J Ind Microbiol, 15, pp.384~390 (1995)[DOI]
  13. Drago L, "Inhibition of in vitro growth of enteropathogens by new Lactobacillus isolates of human intestinal origin", FEMS Microbiol Lett, 153, pp.455~463 (1997)[DOI]
  14. Kolenbrander PE, "Oral microbial communities : biofilms, interactions, and genetic systems", Annu Rev Microbiol, 54, pp.413~437 (2000)[DOI]
  15. Datta A, "Bacterial coaggregation among the most commonly isolated bacteria from contact lens cases", Invest Ophthalmol Vis Sci, 58, pp.50~58 (2017)[DOI]
  16. Afonso AC, "Bacterial coaggregation in aquatic systems", Water Res, 196, pp.117037~ (2021)[DOI]
  17. Rickard AH, "Bacterial coaggregation : an integral process in the development of multi-species biofilms", Trends Microbiol, 11, pp.94~100 (2003)[DOI]
  18. Stevens MR, "Coaggregation occurs between microorganisms isolated from different environments", FEMS Microbiol Ecol, 91, pp.fiv123~ (2015)[DOI]
  19. Bradshaw DJ, "Role of Fusobacterium nucleatum and coaggregation in anaerobe survival in planktonic and biofilm oral microbial communities during aeration", Infect Immun, 66, pp.4729~4732 (1998)[DOI]
  20. Ledder RG, "Coaggregation between and among human intestinal and oral bacteria", FEMS Microbiol Ecol, 66, pp.630~636 (2008)[DOI]
  21. Rickard AH, "Coaggregation between freshwater bacteria within biofilm and planktonic communities", FEMS Microbiol Lett, 220, pp.133~140 (2003)[DOI]
  22. Buswell CM, "Coaggregation amongst aquatic biofilm bacteria", J Appl Microbiol, 83, pp.477~484 (1997)[DOI]
  23. Kolenbrander PE, "Communication among oral bacteria", Microbiol Mol Biol Rev, 66, pp.486~505 (2002)[DOI]
  24. Simões LC, "Acinetobacter calcoaceticus plays a bridging function in drinking water biofilms", Engineering and Innovation at the University of Minho, ~ (2008)
  25. O’Donnell MJ, "Management of dental unit waterline biofilms in the 21st century", Future Microbiol, 6, pp.1209~1226 (2011)[DOI]
  26. Szymańska J, "Microbial contamination of dental unit waterlines", Ann Agric Environ Med, 15, pp.173~179 (2008)
  27. Walker JT, "Microbiological evaluation of a range of disinfectant products to control mixed-species biofilm contamination in a laboratory model of a dental unit water system", Appl Environ Microbiol, 69, pp.3327~3332 (2003)[DOI]
  28. Kolenbrander PE, "Lactose-reversible coaggregation between oral actinomycetes and Streptococcus sanguis", Infect Immun, 33, pp.95~102 (1981)[DOI]
  29. Kolenbrander PE, "Inhibition of coaggregation between Fusobacterium nucleatum and Porphyromonas(Bacteroides)gingivalis by lactose and related sugars", Infect Immun, 57, pp.3204~3209 (1989)[DOI]
  30. Rickard AH, "Coaggregation between aquatic bacteria is mediated by specific-growth-phase-dependent lectin-saccharide interactions", Appl Environ Microbiol, 66, pp.431~434 (2000)[DOI]
  31. Simões LC, "Intergeneric coaggregation among drinking water bacteria : evidence of a role for Acinetobacter calcoaceticus as a bridging bacterium", Appl Environ Microbiol, 74, pp.1259~1263 (2008)[DOI]
  32. Sato T, "Coaggregation between Prevotella oris and Porphyromonas gingivalis", J Microbiol Immunol Infect, 47, pp.182~186 (2014)[DOI]
  33. Yoon HY, "Susceptibility of bacteria isolated from dental unit waterlines to disinfecting chemical agents", J Gen Appl Microbiol, 64, pp.269~275 (2019)[DOI]
  34. Cisar JO, "Specificity of coaggregation reactions between human oral streptococci and strains of Actinomyces viscosus or Actinomyces naeslundii", Infect Immun, 24, pp.742~752 (1979)[DOI]
  35. Min KR, "Physicochemical parameters influencing coaggregation between the freshwater bacteria Sphingomonas natatoria 2. 1 and Micrococcus luteus 2. 13", Biofouling, 26, pp.931~940 (2010)[DOI]
  36. Mutha NVR, "Transcriptional responses of Streptococcus gordonii and Fusobacterium nucleatum to coaggregation", Mol Oral Microbiol, 33, pp.450~464 (2018)[DOI]
  37. Walker JT, "Microbial biofilm formation in DUWS and their control using disinfectants", J Dent, 35, pp.721~730 (2007)[DOI]
  38. Kolenbrander PE, "Multigeneric aggregations among oral bacteria : a network of independent cell-to-cell interactions", J Bacteriol, 168, pp.851~859 (1986)[DOI]
  39. Kolenbrander PE, "Intergeneric coaggregation among human oral bacteria and ecology of dental plaque", Annu Rev Microbiol, 42, pp.627~656 (1988)[DOI]
  40. Lancy P, "Corncob formation between Fusobacterium nucleatum and Streptococcus sanguis", Infect Immun, 40, pp.303~309 (1983)[DOI]
  41. He X, "Adherence to streptococci facilitates Fusobacterium nucleatum integration into an oral microbial community", Microb Ecol, 63, pp.532~542 (2012)[DOI]
  42. Katharios-Lanwermeyer S, "Mini-review : microbial coaggregation : ubiquity and implications for biofilm development", Biofouling, 30, pp.1235~1251 (2014)[DOI]
  43. Krisanaprakornkit S, "Inducible expression of human beta-defensin 2 by Fusobacterium nucleatum in oral epithelial cells : multiple signaling pathways and role of commensal bacteria in innate immunity and the epithelial barrier", Infect Immun, 68, pp.2907~2915 (2000)[DOI]
  44. Darenfed H, "Acquisition of plasmin activity by Fusobacterium nucleatum subsp. nucleatum and potential contribution to tissue destruction during periodontitis", Infect Immun, 67, pp.6439~6444 (1999)[DOI]
  45. Sharon N, "Carbohydrates as future anti-adhesion drugs for infectious diseases", Biochim Biophys Acta, 1760, pp.527~537 (2006)[DOI]
  46. Schel AJ, "Comparison of the efficacies of disinfectants to control microbial contamination in dental unit water systems in general dental practices across the European Union", Appl Environ Microbiol, 72, pp.1380~1387 (2006)[DOI]

관련 논문AI추천

이 간행물의 다른 논문 (19건)