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어성초(魚腥草)의 면역활성에 미치는 영향

Immune Enhancing Effect of Houttuyniae Herba on Mouse Macrophage

임은미|김윤상|김정현
대한한방부인과학회 (The Society Of Oriental Obstetrics & Gynecology)|2012.05|대한한방부인과학회지 (The Journal of Oriental Obstetrics ＆ Gynecology)|제25권 제2호|pp. 12-22|DOI http://dx.doi.org/

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키워드

macrophageNOcytokineHouttuyniae Herbahydrogen peroxide
주제분류한의학

초록

Objectives: The aim of this study is to investigate immune enhancing effect of Houttuyniae Herba water extract(HW) on RAW 264.7 cell of mouse macrophages. Methods: Effects of HW on productions of nitric oxide(NO) and hydrogen peroxide(H2O2) in RAW 264.7 mouse macrophages were measured. Effect of HW on production of cytokines such as interleukin(IL)-1β, IL-6, and tumor necrosis factor(TNF)-α in RAW 264.7 cells was accessed by a multiplex bead array assay based on xMAP technology. All of results were represented P<0.05 compared to the normal. Results:1. After 24 hr incubation, HW increased significantly NO production in RAW 264.7 cells at the concentrations of 25, 50, 100 and 200 ㎍/mL. 2. After 24 hr incubation, HW increased significantly hydrogen peroxide production in RAW 264.7 cells at the concentrations of 25, 50, 100 and 200 ㎍/mL. 3. After 24 hr incubation, HW increased significantly IL-1β production in RAW 264.7 cells at the concentrations of 100 and 200 ㎍/mL. 4. After 24 hr incubation, HW increased significantly IL-6 production in RAW 264.7 cells at the concentrations of 100 and 200 ㎍/mL. 5. After 24 hr incubation, HW increased significantly TNF-α production in RAW 264.7 cells at the concentrations of 50, 100, and 200 ㎍/mL. Conclusions: These results suggest that HW has immune enhancing activity related with its increasement of NO, hydrogen peroxide, IL-1β, IL-6, and TNF-α in macrophages.

Abstract

Objectives: The aim of this study is to investigate immune enhancing effect of Houttuyniae Herba water extract(HW) on RAW 264.7 cell of mouse macrophages. Methods: Effects of HW on productions of nitric oxide(NO) and hydrogen peroxide(H2O2) in RAW 264.7 mouse macrophages were measured. Effect of HW on production of cytokines such as interleukin(IL)-1β, IL-6, and tumor necrosis factor(TNF)-α in RAW 264.7 cells was accessed by a multiplex bead array assay based on xMAP technology. All of results were represented P<0.05 compared to the normal. Results:1. After 24 hr incubation, HW increased significantly NO production in RAW 264.7 cells at the concentrations of 25, 50, 100 and 200 ㎍/mL. 2. After 24 hr incubation, HW increased significantly hydrogen peroxide production in RAW 264.7 cells at the concentrations of 25, 50, 100 and 200 ㎍/mL. 3. After 24 hr incubation, HW increased significantly IL-1β production in RAW 264.7 cells at the concentrations of 100 and 200 ㎍/mL. 4. After 24 hr incubation, HW increased significantly IL-6 production in RAW 264.7 cells at the concentrations of 100 and 200 ㎍/mL. 5. After 24 hr incubation, HW increased significantly TNF-α production in RAW 264.7 cells at the concentrations of 50, 100, and 200 ㎍/mL. Conclusions: These results suggest that HW has immune enhancing activity related with its increasement of NO, hydrogen peroxide, IL-1β, IL-6, and TNF-α in macrophages.

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