어성초 추출물이 지질다당질과 인터페론감마로 활성화된 Raw 264.7 Cells에서의 항염증 효과
Anti-Inflammatory Effect of Houttuynia Cordata Thunb in Activated Raw 264.7 Cells Activated with Lipopolysaccharide and Interferon Gamma
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Background: Houttuynia cordata has long been used in traditional oriental medicine for the treatment of inflammatory disease. In the present study, we investigated the anti-inflammatory effect of Houttuynia cordata extracts on Raw 264.7 cells, which is activated with lipopolysaccharide (LPS) and interferon-γ (IFN-γ). Methods: We treated LPS-and IFN-γ-activated murine macrophage Raw 264.7 cells with extracts of Houttuynia cordata and measured the production of nitric oxide (NO) and prostaglandin E2 (PGE2), and the expression of inducible nitric oxide synthase (iNOS), cyclooxygenase (COX)-2, and pro-inflammatory cytokines (Interleukin [IL]-1α, IL-1β, IL-4, IL-6, IL-10, IL-12, tumor necrosis factor-α, regulated on activation normal T-cell expressed and secreted) in activated Raw 264.7 cells after treatment with Houttuynia cordata extracts. Results: NO formation by activated Raw 264.7 cells was reduced in response to Houttuynia Cordata in a dose-dependent manner. Formation of PGE2, a final product of the COX pathway was reduced by Houttuynia cordata extracts and reduction in COX-2 expression was proportional to the reduction in PGE2. The expression of proteins, iNOS and COX-2 decreased with increasing concentration of Houttuynia cordata extracts. The related pro-inflammatory cytokines did not decrease in the cells treated with Houttuynia cordata extracts. Conclusion: Houttuynia Cordata extracts suppressed the production of NO and PGE2 and decreased the expression of iNOS and COX-2. It has potential for the treatment of inflammatory diseases. Further studies are needed to elucidate the effects of Houttuynia Cordata extracts on the production of inflammatory cytokines.
Abstract
Background: Houttuynia cordata has long been used in traditional oriental medicine for the treatment of inflammatory disease. In the present study, we investigated the anti-inflammatory effect of Houttuynia cordata extracts on Raw 264.7 cells, which is activated with lipopolysaccharide (LPS) and interferon-γ (IFN-γ). Methods: We treated LPS-and IFN-γ-activated murine macrophage Raw 264.7 cells with extracts of Houttuynia cordata and measured the production of nitric oxide (NO) and prostaglandin E2 (PGE2), and the expression of inducible nitric oxide synthase (iNOS), cyclooxygenase (COX)-2, and pro-inflammatory cytokines (Interleukin [IL]-1α, IL-1β, IL-4, IL-6, IL-10, IL-12, tumor necrosis factor-α, regulated on activation normal T-cell expressed and secreted) in activated Raw 264.7 cells after treatment with Houttuynia cordata extracts. Results: NO formation by activated Raw 264.7 cells was reduced in response to Houttuynia Cordata in a dose-dependent manner. Formation of PGE2, a final product of the COX pathway was reduced by Houttuynia cordata extracts and reduction in COX-2 expression was proportional to the reduction in PGE2. The expression of proteins, iNOS and COX-2 decreased with increasing concentration of Houttuynia cordata extracts. The related pro-inflammatory cytokines did not decrease in the cells treated with Houttuynia cordata extracts. Conclusion: Houttuynia Cordata extracts suppressed the production of NO and PGE2 and decreased the expression of iNOS and COX-2. It has potential for the treatment of inflammatory diseases. Further studies are needed to elucidate the effects of Houttuynia Cordata extracts on the production of inflammatory cytokines.
참고문헌 (25)
- 송종호, "어성초(Houttuynia cordata) 뿌리에서 추출한 순차분획물의 항균활성", 한국식품영양과학회지, 32(7), pp.1053~1058 (2003), 한국식품영양과학회
- Chung CK, "Effect of Houttuynia cordata ethanol extracts on serum lipids and antioxidant enzymes in rat fed high fat diet", J Korean Soc Food Sci Nutr, 28, pp.205~211 (1999)
- Choi YH, "Antimutagenic effects of the juice and boiling water extract of Houttuynia cordata Thunb", J Korean Soc Food Sci Nutr, 23, pp.916~921 (1994)
- Chou SC, "The constituents and their bioactivities of Houttuynia cordata", Chem Pharm Bull(Tokyo), 57, pp.1227~1230 (2009)
- Kim GS, "Biological and antibacterial activities of the natural herb Houttuynia cordata water extract against the intracellular bacterial pathogen salmonella within the RAW 264.7 macrophage", Biol Pharm Bull, 31, pp.2012~2017 (2008)
- Lee JS, "Suppressive effects of Houttuynia cordata Thunb (Saururaceae) extract on Th2 immune response", J Ethnopharmacol, 117, pp.34~40 (2008)
- Kumar V, "Pathologic basis of disease. 7th ed", pp.68~73, WB Saunders
- Storck M, "Production of proinflammatory cytokines and adhesion molecules in ex-vivo xenogeneic kidney perfusion", Transpl Int, 7(1), pp.S647~S649 (1994)
- Moncada S, "Nitric oxide: physiology, pathophysiology, and pharmacology", Pharmacol Rev, 43, pp.109~142 (1991)
- Lee AK, "Inhibition of lipopolysaccharide-inducible nitric oxide synthase, TNF-alpha and COX-2 expression by sauchinone effects on I-kappaBalpha phosphorylation, C", EBP and AP-1 activation, 2003, pp.139, 11~20
- Delgado AV, "Production of tumor necrosis factor-alpha, interleukin 1-beta, interleukin 2, and interleukin 6 by rat leukocyte subpopulations after exposure to substance P", Neuropeptides, 37, pp.355~361 (2003)
- Aktan F, "iNOS-mediated nitric oxide production and its regulation", Life Sci, 75, pp.639~653 (2004)
- Liang YC, "Suppression of inducible cyclooxygenase and inducible nitric oxide synthase by apigenin and related flavonoids in mouse macrophages", Carcinogenesis, 20, pp.1945~1952 (1999)
- Greenhough A, "The COX-2", PGE2 pathway: key roles in the hallmarks of cancer and adaptation to the tumour microenvironment, 2009, pp.30, 377~386
- Harasstani OA, "Flavonoid combinations cause synergistic inhibition of proinflammatory mediator secretion from lipopolysaccharide- induced RAW 264.7 cells", Inflamm Res, 59, pp.711~721 (2010)
- 정일홍, "인체유방암세포에서 Cyclooxygenase-2 활성 및 Prostaglandin E2 생성에 미치는 어성초 추출물의 영향", 동의생리병리학회지, 20(3), pp.690~696 (2006), 대한동의병리학회
- Wink DA, "Chemical biology of nitric oxide: insights into regulatory, cytotoxic, and cytoprotective mechanisms of nitric oxide", Free Radic Biol Med, 25, pp.434~456 (1998)
- benze nesulfonamide (SC-58635, celecoxib), "J Med Chem", 1997, 1347~1365
- Hyeong-Jin Kim, "Anti-inflammatory Effect of Quercetin on Picryl Chloride-induced Contact Dermatitis in BALB", c Mice, 2010, pp.26(1), 7~13[DOI]
- Lu HM, "Anti-inflammatory effect of Houttuynia cordata injection", J Ethnopharmacol, 104, pp.245~249 (2006)
- Prasit P, "Chapter21. Selective Cyclooxygenase-2 Inhibitors", Annual Reports in Medicinal Chemistry, 32, pp.211~220 (1997)
- Lazer ES, "Antiinflammatory 2-benzyl-4-sulfonyl-4H-isoquinoline- 1,3-diones: novel inhibitors of COX-2", Bioorg Med Chem Lett, 8, pp.1181~1186 (1998)
- Coruzzi G, "Gastrointestinal safety of novel nonsteroidal antiinflammatory drugs: selective COX-2 inhibitors and beyond", Acta Biomed, 78, pp.96~110 (2007)
- Li W, "Houttuynia cordata, a novel and selective COX-2 inhibitor with anti-inflammatory activity", J Ethnopharmacol, 133, pp.922~927 (2011)
- Tang YJ, "Houttuynia cordata Thunb extract induces apoptosis through mitochondrial-dependent pathway in HT-29 human colon adenocarcinoma cells", Oncol Rep, 22, pp.1051~1056 (2009)