하고초 열수추출물이 대식세포 면역만응에 미치는 영향
Effects of Prunellae Spica Water Extract on Immune Response in Macrophage Cells
1
조회
0
다운로드
19
인용
0
참고문헌
키워드
초록
Purpose: The purpose of this study was to investigate the effects of Prunellae Spica Water Extract(PSE) on immune response in macrophage cells. Methods: We had devided two group the one is normal group; not treated with PSE, and the other is experimental group; treated with PSE. We measured the cell viability of PSE on RAW 264.7 cells and investigated production of nitric oxide(NO) and cytokines such as interleukin(IL)-1β, IL-6 and tumor necrosis factor (TNF)-α with sample PSE. Results:1. Cell viability of PSE on RAW 264.7 cells was significantly decreased in both 24 hr and 48 hr incubation. 2. NO production of PSE on RAW 264.7 cells was significantly increased in both 24 hr and 48 hr incubation. 3. IL-1β production of PSE on RAW 264.7 cells was significantly increased under concentration over 50 ㎍/㎖ in 24 hr incubation. 4. IL-6 production of PSE on RAW 264.7 cells was significantly increased under concentration over 50 ㎍/㎖ in 24 hr incubation. 5. TNF-α production of PSE on RAW 264.7 cells was significantly increased under concentration over 50 ㎍/㎖ in 24 hr incubation. Conclusion: NO, IL-1β, IL-6 and TNF-α production of PSE on RAW 264.7 cells was significantly increased. This study suggest that PSE stimulates the macrophage and enhances the immune response.
Abstract
Purpose: The purpose of this study was to investigate the effects of Prunellae Spica Water Extract(PSE) on immune response in macrophage cells. Methods: We had devided two group the one is normal group; not treated with PSE, and the other is experimental group; treated with PSE. We measured the cell viability of PSE on RAW 264.7 cells and investigated production of nitric oxide(NO) and cytokines such as interleukin(IL)-1β, IL-6 and tumor necrosis factor (TNF)-α with sample PSE. Results:1. Cell viability of PSE on RAW 264.7 cells was significantly decreased in both 24 hr and 48 hr incubation. 2. NO production of PSE on RAW 264.7 cells was significantly increased in both 24 hr and 48 hr incubation. 3. IL-1β production of PSE on RAW 264.7 cells was significantly increased under concentration over 50 ㎍/㎖ in 24 hr incubation. 4. IL-6 production of PSE on RAW 264.7 cells was significantly increased under concentration over 50 ㎍/㎖ in 24 hr incubation. 5. TNF-α production of PSE on RAW 264.7 cells was significantly increased under concentration over 50 ㎍/㎖ in 24 hr incubation. Conclusion: NO, IL-1β, IL-6 and TNF-α production of PSE on RAW 264.7 cells was significantly increased. This study suggest that PSE stimulates the macrophage and enhances the immune response.
참고문헌 (35)
- 韓方婦人科學會 敎材編纂委員會, "韓醫婦人科學(上)", pp.62~ (2002), 정담
- 韓醫科大學 本草學 編纂委員會, "本草學", pp.211~212 (2004), 영림사
- 김창민, "完譯 中藥大辭典", pp.4604~4608 (1997), 정담
- 송봉근, "수종 한약재가 면역 반응에 미치는 영향", 대한한의학회지, 18(2), pp.43~57 (1997)
- Ferrari M, "MTT colorimetric assay for testing macrophage cytotoxic activity in vitro", J Immunol Methods, 131(2), pp.165~172 (1990)
- Weissman BA, "Measurement of NO and NO synthase", Curr Protoc Neurosci, 7(7) (2001)
- Giustarini D, "Nitrite and nitrate measurement by Griess reagent in human plasma: evaluation of interferences and standardization", Methods Enzymol, 440, pp.361~380 (2008)
- Politch JA, "Concentrations and significance of cytokines and other immunologic factors in semen of healthy fertile men", Hum Reprod, 22(11), pp.2928~2935 (2007)
- 中島泉, "新免疫入門學", pp.54~55 (1997), 지구문화사
- 정태호, "최신 면역학 강의", pp.211~ (1996), 경북대학교출판부
- 이연대, "최신면역학", pp.33~ (1985), 집문당
- 楊維傑, "黃帝內經素問", pp.3~266 (1991), 國風出版社
- 洪元植, "精矯 黃帝內經靈樞", pp.286~ (1985), 동양의학연구원
- 강대원, "固眞飮子가 methotrexate로 유발된 흰쥐의 免役機能低下에 미치는 影響(영향)", 대한한방내과학회지, 25(4), pp.117~128 (2004), 대한한방내과학회
- 서형호, "金水六君煎이 마우스 면역반응에 미치는 영향", 대한한방내과학회지, (1), pp.89~99 (2005)
- 송봉근, "수종 한약재가 면역 반응에 미치는 영향", 대한한의학회지, 18(2), pp.43~57 (1997)
- 채우석, "면역질환의 한방개념과 치료에 관한 문헌적 고찰", 대한한의학회지, 11(2), pp.54~91 (1991)
- 李梴, "原本 編註醫學入門(下)", pp.1615~ (2002), 남산당
- 許浚, "對譯 東醫寶鑑", pp.1816~ (2005), 동의보감출판사
- 박신화, "댑싸리 하고초 약침액의 암예방 활성", 대한경락경혈학회지, 18(1), pp.11~20 (2001)
- 허자경, "免疫活性에 의한 夏枯草의 癌轉移 抑制 效果", 대한한방부인과학회지, 22(1), pp.41~52 (2009), 대한한방부인과학회
- 정병운, "夏枯草가 고지방 식이에 의해 유발된 대뇌신경세포의 세포사에 미치는 영향", 대한한방내과학회지, 23(3), pp.386~396 (2002), 대한한방내과학회
- 임사비나, "꿀풀과 夏枯草藥鍼이 甲狀腺機能亢進症에 미치는 影響", 대한한의학회지, 18(1), pp.399~410 (1997)
- 이윤섭, "꿀풀과 및 댑싸리 夏枯草藥鍼이 甲狀腺機能亢進症에 미치는 影響", 대한침구학회지, 14(1), pp.494~502 (1997)
- 임은미, "여성본초학", pp.308~ (2005), 전국의학사
- 남경수, "하고초 에탄올추출물이 유방암 예방효소계에 미치는 영향", 생약학회지, 34(2), pp.161~165 (2003), 한국생약학회
- 홍은숙, "하고초 추출물의 항노화 효과에 관한 연구", 대한화장품학회지, 34(2), pp.129~135 (2008), 대한화장품학회
- Newman SL, "Macrophages in host defense against Histoplasma capsulatum", Trends Microbiol, 7(2), pp.67~71 (1999)
- Morrison DC, "Endotoxins and disease mechanisms", Annu Rev Med, 38, pp.417~432 (1987)
- Tracey KJ, "Anti-cachectin", TNF monoclonal antibodies prevent septic shock during lethal bacteraemia, 1987, pp.330, 662~664
- 이귀녕, "임상병리파일", pp.717~754 (2003), 의학문화사
- 대한내과학회, "HARRISON'S 내과학(2)", pp.1861~ (2004), MIP
- 대한진단검사의학회, "진단검사의학", pp.429~433 (2001), 고려의학
- Kishimoto T, "The biology of interleukin-6", Blood, 74(1), pp.1~10 (1989)
- 대한진단검사의학회, "임상병리학", pp.429~433 (2001), 고려의학