連翹金貝煎의 Escherichia coli에 대한 시험관내 항균력 평가
In Vitro Antibacterial Effects of Yeonkyokeumpae-jeon against Escherichia coli
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Objectives: The object of this study was to observe the in vitro antibacterial effectsof Yeonkyokeumpae-jeon (YKKPJ) have been used for treating various gynecologicaldiseases including mastitis in Korea, and individual six kinds of herbal compositionaqueous extracts - Forsythiae Fructus (FF), Millettiae Caulis (MC), Lonicerae Flos(LF), Fritillaria Thunbergii Bulb (FT), Taraxci Herba (TH) and Prunellae Spica(PS) against E. coli. Methods: Antibacterial activities against E. coli of YKKPJ, FF, MC, LF, FT,TH and PS aqueous extracts were detected using standard agar microdilution methods. In addition, the effects on the bacterial growth curve were also monitored at MICand MIC×2 levels. The effects on the intracellular killing and bacterial invasionof individual test materials were also observed using Raw 264.7 and MCF-7. Theresults were compared with ciprofloxacin, a second generation of quinolone antibioticsin the present study. Results: MIC of YKKPJ, FF, MC, LF, FT, TH, PS aqueous extracts againstE. coli were detected as 0.039±0.013 mg/ml, 0.064±0.033 mg/ml, 0.108±0.053 mg/ml,0.078±0.027 mg/ml, 16.250±8.385 mg/ml, 15.625±9.375 mg/ml, 0.254±0.131 mg/ml,repectively. YKKPJ, FF, MC, LF, FT, TH, PS aqueous extracts showed antibacterialeffects against to E. coli, except for FT and TH, which were showed negligibleantibacterial effects, respectively. In addition, ciprofloxacin with YKKPJ, FF, MC,LF and PS aqueous extracts also showed marked dosage-dependent inhibition of bacterialgrowth, and favorable inhibitory effects on the both bacterial invasion and intracellularkilling assays using MCF-7 and Raw 264.7 cells were detected in this experiment. Conclusions: The results obtained in this study suggest that traditional polyherbalformula YKKPJ aqueous extracts showed more favorable antibacterial activitiesas compared to individual six kinds of herbal composition aqueous extracts. Theantibacterial effects of YKKPJ against E. coli considered as results of complicatedsynergic effects of their six kinds of herbal components rather than simple antibacterialeffects of single herbal components. It means, YKKPJ aqueous extracts may showpotent anti-infectious effects against E. coil for mastitis.
Abstract
Objectives: The object of this study was to observe the in vitro antibacterial effectsof Yeonkyokeumpae-jeon (YKKPJ) have been used for treating various gynecologicaldiseases including mastitis in Korea, and individual six kinds of herbal compositionaqueous extracts - Forsythiae Fructus (FF), Millettiae Caulis (MC), Lonicerae Flos(LF), Fritillaria Thunbergii Bulb (FT), Taraxci Herba (TH) and Prunellae Spica(PS) against E. coli. Methods: Antibacterial activities against E. coli of YKKPJ, FF, MC, LF, FT,TH and PS aqueous extracts were detected using standard agar microdilution methods. In addition, the effects on the bacterial growth curve were also monitored at MICand MIC×2 levels. The effects on the intracellular killing and bacterial invasionof individual test materials were also observed using Raw 264.7 and MCF-7. Theresults were compared with ciprofloxacin, a second generation of quinolone antibioticsin the present study. Results: MIC of YKKPJ, FF, MC, LF, FT, TH, PS aqueous extracts againstE. coli were detected as 0.039±0.013 mg/ml, 0.064±0.033 mg/ml, 0.108±0.053 mg/ml,0.078±0.027 mg/ml, 16.250±8.385 mg/ml, 15.625±9.375 mg/ml, 0.254±0.131 mg/ml,repectively. YKKPJ, FF, MC, LF, FT, TH, PS aqueous extracts showed antibacterialeffects against to E. coli, except for FT and TH, which were showed negligibleantibacterial effects, respectively. In addition, ciprofloxacin with YKKPJ, FF, MC,LF and PS aqueous extracts also showed marked dosage-dependent inhibition of bacterialgrowth, and favorable inhibitory effects on the both bacterial invasion and intracellularkilling assays using MCF-7 and Raw 264.7 cells were detected in this experiment. Conclusions: The results obtained in this study suggest that traditional polyherbalformula YKKPJ aqueous extracts showed more favorable antibacterial activitiesas compared to individual six kinds of herbal composition aqueous extracts. Theantibacterial effects of YKKPJ against E. coli considered as results of complicatedsynergic effects of their six kinds of herbal components rather than simple antibacterialeffects of single herbal components. It means, YKKPJ aqueous extracts may showpotent anti-infectious effects against E. coil for mastitis.
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