본문 바로가기
KERD
논문KCI 등재

加減攝營煎의 Gardnerella vaginalis에 대한 시험관내 항균력 및 Clindamycin과의 병용효과

In Vitro Antibacterial Effects of Gagam-seopyoungjeon Aqueous Extracts and Their Combination Effects with Clindamycin against Gardnerella Vaginalis

김동철|오자영
대한한방부인과학회 (The Society Of Oriental Obstetrics & Gynecology)|2014.02|대한한방부인과학회지 (The Journal of Oriental Obstetrics ＆ Gynecology)|제27권 제1호|pp. 65-80|DOI http://dx.doi.org/10.15204/jkobgy2014.27.1.065

2

조회

0

다운로드

2

인용

0

참고문헌

키워드

VaginitisClindamycinGardnerella VaginalisGagam-seopyoungjeonVK2 Cell
주제분류한의학

초록

Objectives: The object of this study was to observe the in vitro antibacterialeffects of Gagam-seopyoungjeon aqueous extracts (GGSYJ) against Gardnerellavaginalis and the possible synergic combination effects with clindamycin. Methods: Antibacterial activities against Gardnerella vaginalis of GGSYJ weredetected using minimal inhibition concentration (MIC), and the effects on the bacterialgrowth curve were also monitored at MIC and MIC×2 levels. The combinationeffects of GGSYJ with clindamycin were observed by checkboard microtiter assay,and the effects of bacterial growth curve treated with GGSYJ MIC+clindamycinMIC, 1/2 MIC and 1/4 MIC, respectively. The effects on the bacterial invasion andintracellular killing of GGSYJ were also observed using human vaginal epithelial(VK2) and murine macrophage (Raw264.7) cells with combination effects withclindamycin after treatment of GGSYJ MIC+clindamycin 1/2 MIC, 1/4 MIC and1/6 MIC, respectively. Results: The MIC of clindamycin and GGSYJ against Gardnerella vaginaliswere detected as 0.012±0.006 (0.004~0.016) μg/ml and 1.016±0.524 (0.391~1.563)mg/ml, respectively. Clindamycin and GGSYJ were also showed marked dosagedependentinhibition of bacterial growth, and significant decreases of viable cells weredetected in clindamycin MIC+GGSYJ MIC and clindamycin 1/2 MIC+GGSYJMIC treatment as compared with each of single clindamycin MIC and GGSYJMIC treatments. And significant decreases of intraepithelial and intra-macrophageviable bacteria numbers were detected in clindamycin 1/2 MIC+GGSYJ 1/2 MICand clindamycin 1/4 MIC+GGSYJ 1/2 MIC treatment as compared with eachof single clindamycin GGSYJ 1/2 MIC treatments, respectively. Conclusions: GGSYJ showed slight antibacterial effects against Gardnerellavaginalis, but they showed dosage-dependent inhibitory effects on the bacterial growthand VK2 epithelial invasions of bacteria with favorable accelerating effects of intracellularkilling activities of macrophages. In addition, combination of GGSYJ also increasedthe inhibitory effects of clindamycin on the epithelial invasions of Gardnerella vaginalisand intracellular killing activities of macrophages against Gardnerella vaginalis as2-fold higher as compared with clindamycin single treatment, respectively. Therefore,we expected that the clinical dosages of clindamycin can be reduced as 1/2 levelsas combination with GGSYJ.

Abstract

Objectives: The object of this study was to observe the in vitro antibacterialeffects of Gagam-seopyoungjeon aqueous extracts (GGSYJ) against Gardnerellavaginalis and the possible synergic combination effects with clindamycin. Methods: Antibacterial activities against Gardnerella vaginalis of GGSYJ weredetected using minimal inhibition concentration (MIC), and the effects on the bacterialgrowth curve were also monitored at MIC and MIC×2 levels. The combinationeffects of GGSYJ with clindamycin were observed by checkboard microtiter assay,and the effects of bacterial growth curve treated with GGSYJ MIC+clindamycinMIC, 1/2 MIC and 1/4 MIC, respectively. The effects on the bacterial invasion andintracellular killing of GGSYJ were also observed using human vaginal epithelial(VK2) and murine macrophage (Raw264.7) cells with combination effects withclindamycin after treatment of GGSYJ MIC+clindamycin 1/2 MIC, 1/4 MIC and1/6 MIC, respectively. Results: The MIC of clindamycin and GGSYJ against Gardnerella vaginaliswere detected as 0.012±0.006 (0.004~0.016) μg/ml and 1.016±0.524 (0.391~1.563)mg/ml, respectively. Clindamycin and GGSYJ were also showed marked dosagedependentinhibition of bacterial growth, and significant decreases of viable cells weredetected in clindamycin MIC+GGSYJ MIC and clindamycin 1/2 MIC+GGSYJMIC treatment as compared with each of single clindamycin MIC and GGSYJMIC treatments. And significant decreases of intraepithelial and intra-macrophageviable bacteria numbers were detected in clindamycin 1/2 MIC+GGSYJ 1/2 MICand clindamycin 1/4 MIC+GGSYJ 1/2 MIC treatment as compared with eachof single clindamycin GGSYJ 1/2 MIC treatments, respectively. Conclusions: GGSYJ showed slight antibacterial effects against Gardnerellavaginalis, but they showed dosage-dependent inhibitory effects on the bacterial growthand VK2 epithelial invasions of bacteria with favorable accelerating effects of intracellularkilling activities of macrophages. In addition, combination of GGSYJ also increasedthe inhibitory effects of clindamycin on the epithelial invasions of Gardnerella vaginalisand intracellular killing activities of macrophages against Gardnerella vaginalis as2-fold higher as compared with clindamycin single treatment, respectively. Therefore,we expected that the clinical dosages of clindamycin can be reduced as 1/2 levelsas combination with GGSYJ.

참고문헌 (46)

  1. Aroutcheva AA, "Gardnerella vaginalis isolated from patients with bacterial vaginosis and from patients with healthy vaginal ecosystems", Clin Infect Dis, 33(7), pp.1022~1027 (2001)
  2. 한방여성의학 편찬위원회, "한방여성의학", pp.282~293 (2007), 정담
  3. 대한산부인과학회, "부인과학", pp.147~149 (1987), 고려의학
  4. Hauth JC, "Reduced incidence of preterm delivery with metronidazole and erythromycin in women with bacterial vaginosis", N Engl J Med, 333(26), pp.1732~1736 (1995)
  5. Mayaud R, "Tackling bacterial vaginosis in developing countries", Lancet, 350, pp.530~531 (1997)
  6. Hillier SL, "Clindamycin treatment of bacterial vaginosis", Rev Contemp Pharmacother, 3, pp.263~268 (1992)
  7. 조성남, "질염", 대한산부인과학회 연수 강좌, 33, pp.77~85 (2004)
  8. Thomas C, "Antibiotics and hospital-acquired Clostridium difficileassociated diarrhoea: a systematic review", J Antimicrob Chemother, 51(6), pp.1339~1350 (2003)
  9. 김연희, "Gardnerella vaginalis에 대한 한약재의 황균활성", 한국미생물·생명공학회지, 34(1), pp.70~73 (2006), 한국미생물·생명공학회
  10. 葛洪, "肘後備急方", pp.108~ (1996), 人民衛生出版社
  11. 김영훈, "청강의감", 성보사, pp.387~389 (2001)
  12. 조형래, "청강의감 부인과 질환 및 수재 처방에 대한 연구", 대한한방부인과학회지, 13(2), pp.295~325 (2000)
  13. Pfaller MA, "Standardized susceptibility testing of fluconazole:an international collaborative study", Antimicrob Agents Chemother, 36(9), pp.1805~1809 (1992)
  14. Pfaller MA, "Collaborative investigation of variables in susceptibility testing of yeasts", Antimicrob Agents Chemother, 34(9), pp.1648~1654 (1990)
  15. Tenover FC, "Characterization of staphylococci with reduced susceptibilities to vancomycin and other glycopeptides", J Clin Microbiol, 36(4), pp.1020~1027 (1998)
  16. Pfaller MA, "Multicenter evaluation of four methods of yeast inoculum preparation", J Clin Microbiol, 26(8), pp.1437~1441 (1988)
  17. Janssen AM, "Antimicrobial activity of essential oils: 1976-86 literature review. Aspects of the test methods", Planta Med, 53(5), pp.395~398 (1987)
  18. Isenberg HD, "Clin Microbiol procedure manual", pp.1~28 (1992), American Society for Microbiology
  19. 윤정숙, "다제내성 Pseudomonas aeruginosa 균주의 항균제 병합효과", Annals of Clinical Microbiology, 9(1), pp.1~6 (2006), 대한임상미생물학회
  20. Cuffini, "Enhanced Staphylococcus aureus susceptibility to immune-defense induced by subinhibitory and bactericidal concentration of imipenem", J Antimicrob Chemotherp, 31(4), pp.559~568 (1993)
  21. Almeida, "Staphylococcus aureus invasion of bovine mammary epithelial cells", J Dairy Sci, 79(6), pp.1021~1026 (1996)
  22. Hay PE, "Therapy of bacterial vaginosis", J Antimicrob Chemother, 41(1), pp.6~9 (1998)
  23. Bannatyne RM, "Recurrent bacterial vaginosis and metronidazole resistance in Gardnerella vaginalis", Sex Transm Infect, 74(6), pp.455~456 (1998)
  24. Daum RS, "Clinical practice. Skin and soft-tissue infections caused by methicillin-resistant Staphylococcus aureus", N Engl J Med, 357(4), pp.380~390 (2007)
  25. Starr J, "Clostridium difficile associated diarrhoea: diagnosis and treatment", BMJ, 331(7515), pp.498~501 (2005)
  26. Ajantha GS, "Phenotypic detection of inducible clindamycin resistance among Staphylococcus aureus isolates by using the lower limit of recommended inter-disk distance", Indian J Pathol Microbiol, 51(3), pp.376~378 (2008)
  27. 黃度淵, "醫宗損益", pp.50~93 (1993), 여강출판사
  28. 성현아, "蛇床子洗方의 Gardnerella vaginalis에 대한 시험관 내 항균력 및 Metronidazole과 병용효과", 대한한방부인과학회지, 23(2), pp.20~37 (2010), 대한한방부인과학회
  29. 권지명, "淸帶湯의 Gardnerella vaginalis에 대한 시험관내 항균력 및 Clindamycin과의 병용효과", 대한한방부인과학회지, 24(2), pp.1~12 (2011), 대한한방부인과학회
  30. 이승혜, "完帶湯의 Gardnerella vaginalis에 대한 시험관내 항균력 및 Clindamycin과 병용효과", 대한한방부인과학회지, 25(1), pp.34~46 (2012), 대한한방부인과학회
  31. 한영실, "백작약으로부터 식품부패 미생물에 대한 항균성 물질의 분리 및 동정", 한국조리과학회지, 16(5), pp.67~74 (2000)
  32. 최은영, "백출 추출물의 세포독성과 항균효과검색", 동의생리병리학회지, 16(2), pp.23~352 (2002), 대한동의병리학회
  33. 손호용, "재배마 (Dioscorea batatas Decne)의 주아로부터 분리된 phenanthrene 유도체의 항진균 활성", 생명과학회지, 16(4), pp.647~652 (2006), 한국생명과학회
  34. 김현정, "속단추출물의 첨가로 인한 양념 돈육의 품질특성 및 저장성 향상", 한국식품영양과학회지, 40(1), pp.102~109 (2011), 한국식품영양과학회
  35. 정동선, "천궁(Cnidium officinale Makino) 지상부(잎과 줄기) 추출물의 항균활성", 한국미생물·생명공학회지, 35(1), pp.30~35 (2007), 한국미생물·생명공학회
  36. 임성민, "膣炎에 대한 訶子, 車前子, 川芎, 蒲公英, 黃芩의 效果", 대한한방부인과학회지, 17(4), pp.34~45 (2004), 대한한방부인과학회
  37. 유시용, "당귀와 백지로부터 분리한 Coumarin계 물질들의 식물병원균에 대한 항균활성", 한국농약과학회지, 5(3), pp.26~35 (2001)
  38. 윤경원, "당귀속 2종 식물의 항균활성", 한국자원식물학회지, 17(3), pp.278~282 (2004), 한국자원식물학회
  39. Fukai T, "Antimicrobial activity of licorice flavonoids against methicillin -resistant Staphylococcus aureus", Fitoterapia, 73(6), pp.536~539 (2002)
  40. He J, "Antibacterial compounds from Glycyrrhiza uralensis", J Nat Prod, 69(1), pp.121~124 (2006)
  41. Yang C, "Determination of glycyrrhizic acid in different decoctions of sanaotang by HPLC and comparison with antifugal effects in vitro", Zhongguo Zhong Yao Za Zhi, 32(11), pp.1031~1034 (2007)
  42. Braga LC, "Synergic interaction between pomegranate extract and antibiotics against Staphylococcus aureus", 51(7), pp.541~547 (2005)
  43. 최장기, "In Vitro Activity of Methyl Gallate Isolated from Galla Rhois Alone and in Combination with Ciprofloxacin Against Clinical Isolates of Salmonella", Journal of Microbiology and Biotechnology, 18(11), pp.1848~1852 (2008), 한국미생물·생명공학회
  44. Shahverdi AR, "Galbanic acid from Ferulaszowitsiana enhanced the antibacterial activity of penicillin G and cephalexin against Staphylococcus aureus", Biol Pharm Bull, 30(9), pp.1805~1807 (2007)
  45. Muli F, "Use of a continuous-culture biofilm system to study the antimicrobial susceptibilities of Gardnerella vaginalis and Lactobacillus acidophilus", Antimicrob Agents Chemother, 42(6), pp.1428~1432 (1998)
  46. Zhang L, "High-throughput synergy screening identifies microbial metabolites as combination agents for the treatment of fungal infections", Proc Natl Acad Sci USA, 104(11), pp.4606~4611 (2007)

관련 논문AI추천

淸帶湯의 Gardnerella vaginalis에 대한 시험관내 항균력 및 Clindamycin과의 병용효과권지명, 김동철대한한방부인과학회 · 대한한방부인과학회지2011 · 제24권 제2호完帶湯의 Gardnerella vaginalis에 대한 시험관내 항균력 및 Clindamycin과 병용효과김동철, 이승혜대한한방부인과학회 · 대한한방부인과학회지2012 · 제25권 제1호蛇床子洗方의 Gardnerella vaginalis에 대한 시험관 내 항균력 및 Metronidazole과 병용효과김동철, 박영선, 성현아대한한방부인과학회 · 대한한방부인과학회지2010 · 제23권 제2호Gardnerella vaginalis에 대한 한약재의 황균활성김연희, 이흥식한국미생물·생명공학회 · 한국미생물·생명공학회지2006 · 제34권 제1호帶下治療에 使用되는 溫裏藥이 膣內 微生物에 미치는 影響박준홍, 이경섭, 이진무, 이창훈, 장준복, 조정훈대한한방부인과학회 · 대한한방부인과학회지2007 · 제20권 제2호連翹金貝煎의 Escherichia coli에 대한 시험관내 항균력 평가김동철, 한상겸대한한방부인과학회 · 대한한방부인과학회지2015 · 제28권 제1호

이 간행물의 다른 논문 (14건)

梔子가 대식세포의 Cytokine에 미치는 영향임은미, 차지혜pp. 1-16虎杖根의 파골세포 분화 억제 효과와 기전 연구이창훈, 황덕상, 이경섭, 장희재, 이진무, 장준복pp. 17-27소복축어탕의 마우스 경구 단회투여 독성 평가김동철pp. 28-40滋陰降火湯이 Propylthiouracil(PTU)로 유발된 Rat의 갑상선기능저하증에 미치는 영향김석중, 김동철pp. 41-64일부 殺蟲解毒類 한약의 Staphylococcus aureus에 대한 시험관 내 항균 및 항염 효과장세란, 김동철pp. 81-100
원문 보기