黃連解毒湯, 黃連, 黃芩, 黃柏, 梔子의 Staphylococcus aureus에 대한 항균효과
Antimicrobial Activity of Hwangryunheadok-tang, and Scutellariae Radix Extract, Phellodendri Cortex Extract, Coptis Rhizoma Extract, Gardenia Jasminoides Extract against Staphylococcus aureus
2
조회
0
다운로드
3
인용
0
참고문헌
키워드
초록
Objectives: This experiment was done for investigating antimicrobial activity of Hwangryunheadok-tang and Scutellariae Radix extract, Phellodendri Cortex extract, Coptis Rhizoma extract, Gardenia Jasminoides extract against Staphylococcus aureus. Methods: After administering S. aureus on a bacterial culture media plate, antimicrobial activity was tested by dripping 80 μl diluted Hwangryunheadok-tang and Scutellariae Radix extract, Phellodendri Cortex extract, Coptis Rhizoma extract, and Gardenia Jasminoides extract (100%, 50%, 10%, 1%) on plates that were cultivated for a span of time from 16 to 72 hours. Also, minimal inhibitory concentration (MIC) was tested by dripping the minimum dilution density solution that has antimicrobial activity between 80 μl and 20 μl (80 μl, 60 μl, 40 μl, 20 μl) in measure of density. Antimicrobial activity of Hwangryunheadok-tang and four kinds of extracts against S. aureus was continually displayed. Results:1. S. aureus (Standard Microorganism, ATCC)(1) Antimicrobial activity was displayed for Hwangryunheadok-tang, Scutellariae Radix extract, and Phellodendri Cortex extract respectively in the undiluted solution and 50% of diluted magnification. Gardenia Jasminoides extract showed its activity only in the undiluted solution; Coptis Rhizoma extract showed its activity down to 10% of diluted magnification. The antimicrobial activity of the undiluted solution was increased when the volume of inoculation increased. But, there was no difference when time was extended for cultivation. (2) MIC of Hwangryunheadok-tang, Scutellariae Radix extract was 50%, 20 μl. Coptis Rhizoma extract was 10%, 20 μl, Phellodendri Cortex extract was 50%, 80 μl and Gardenia Jasminoides extract was 100%, 60 μl. 2. S. aureus isolated from diarrheal patients(1) When compared to standard microorganism, MIC has decreased. As a result, their antimicrobial activity has increased. (2) Antimicrobial activity of Hwangryunheadok-tang and four extracts was continually shown in extending of the time, 16, 24 and 72 hours. Conclusions: The author comes to the conclusion that Hwangryunheadok-tang, and four kinds of extracts have antimicrobial activity against S. aureus. Especially, when comparing standard microorganisms with S. aureus isolated from diarrheal patients, some cases showed that antimicrobial activity of all the extracts were better than antibiotics. Thus, if a further study is performed, the use of Hwangryunheadok-tang, and four kinds of extracts will be valuable and beneficial in clinical treatments.
Abstract
Objectives: This experiment was done for investigating antimicrobial activity of Hwangryunheadok-tang and Scutellariae Radix extract, Phellodendri Cortex extract, Coptis Rhizoma extract, Gardenia Jasminoides extract against Staphylococcus aureus. Methods: After administering S. aureus on a bacterial culture media plate, antimicrobial activity was tested by dripping 80 μl diluted Hwangryunheadok-tang and Scutellariae Radix extract, Phellodendri Cortex extract, Coptis Rhizoma extract, and Gardenia Jasminoides extract (100%, 50%, 10%, 1%) on plates that were cultivated for a span of time from 16 to 72 hours. Also, minimal inhibitory concentration (MIC) was tested by dripping the minimum dilution density solution that has antimicrobial activity between 80 μl and 20 μl (80 μl, 60 μl, 40 μl, 20 μl) in measure of density. Antimicrobial activity of Hwangryunheadok-tang and four kinds of extracts against S. aureus was continually displayed. Results:1. S. aureus (Standard Microorganism, ATCC)(1) Antimicrobial activity was displayed for Hwangryunheadok-tang, Scutellariae Radix extract, and Phellodendri Cortex extract respectively in the undiluted solution and 50% of diluted magnification. Gardenia Jasminoides extract showed its activity only in the undiluted solution; Coptis Rhizoma extract showed its activity down to 10% of diluted magnification. The antimicrobial activity of the undiluted solution was increased when the volume of inoculation increased. But, there was no difference when time was extended for cultivation. (2) MIC of Hwangryunheadok-tang, Scutellariae Radix extract was 50%, 20 μl. Coptis Rhizoma extract was 10%, 20 μl, Phellodendri Cortex extract was 50%, 80 μl and Gardenia Jasminoides extract was 100%, 60 μl. 2. S. aureus isolated from diarrheal patients(1) When compared to standard microorganism, MIC has decreased. As a result, their antimicrobial activity has increased. (2) Antimicrobial activity of Hwangryunheadok-tang and four extracts was continually shown in extending of the time, 16, 24 and 72 hours. Conclusions: The author comes to the conclusion that Hwangryunheadok-tang, and four kinds of extracts have antimicrobial activity against S. aureus. Especially, when comparing standard microorganisms with S. aureus isolated from diarrheal patients, some cases showed that antimicrobial activity of all the extracts were better than antibiotics. Thus, if a further study is performed, the use of Hwangryunheadok-tang, and four kinds of extracts will be valuable and beneficial in clinical treatments.
참고문헌 (33)
- Oldfield EC, "Emerging foodborne pathogens: keeping your patients and your families safe", Rev Gastroenterol Disorders, 1(4), pp.177~186 (2001)
- 송유진, "김치에서 분리한 세균인 Lactobacillus plantarum YK-9의 식중독 원인세균에 대한 항균활성 및 특성", KSBB Journal, 24(3), pp.273~278 (2009), 한국생물공학회
- 이종경, "최근 2년간(2009-2010) 우리나라 병원성 대장균 식중독 역학조사 보고서 분석", 한국식품위생안전성학회지, 27(4), pp.366~374 (2012), 한국식품위생안전성학회
- Hiramatsu K, "Dissemination in Japanese hospitals of strains of Staphylococcus aureus heterogeneously resistant to vancomycin", Lancet, 350(9092), pp.1670~1673 (1997)
- 손동화, "한약재의 식중독균에 대한 항균효과", J Korean Soc. Hygienic Sciences, 7(2), pp.102~108 (2001)
- 박욱연, "한약재 추출물의 항균효과 검색", J Korean Soc. Food Nutr, 21(1), pp.91~96 (2001)
- 방제학, "방제학", pp.180~ (1999), 영림사
- 성인화, "黃芩과 黃柏 추출물의 MRSA와 Candida에 대한 항균활성", 미생물학회지, 40(1), pp.17~22 (2004), 한국미생물학회
- 배지현, "연교 추출물의 항균활성 검색 및 일부 식중독균의 증식에 미치는 영향", 한국식품조리과학회지, 21(3), pp.319~325 (2005), 한국식품조리과학회
- 이전우, "黃連 추출물의 항염효능에 관한 연구", 대한본초학회지, 29(5), pp.83~90 (2014), 대한본초학회
- 이종구, "황백과 연교로부터 분리된 생리 활성물질 및 그들의 melanogenesis 저해효과와 항염증작용", 박사, 약학, pp.2008
- 고광묵, "치자 엑스의 가수분해에 의한 피부투과 및 항염효과", 박사, 약학, pp.2002
- 류응주, "梔子추출물의 항균특성 및 안전성 검사", J Agriculture & Life Sciences, 38(4), pp.11~19 (2004)
- 도은수, "일황련 및 황백나무 추출액의 항균활성", Korean J Plant Res, 10(4), pp.351~359 (1997)
- 윤인숙, "마늘추출물의 식중독균에 대한 항균검사", 한국콘텐츠학회 논문지, 9(2), pp.339~349 (2009), 한국콘텐츠학회
- 홍경현, "오미자 추출액 첨가 요구르트의 식중독균 증식 억제 효과", 한국축산식품학회지, 23(4), pp.342~349 (2003), 한국축산식품학회
- 이영은, "국내산 무화과의 식중독균에 대한 항균활성", 한국식품조리과학회지, 21(1), pp.84~93 (2005), 한국식품조리과학회
- 이신호, "두부 부패 미생물과 병원성 미생물에 대한 황금의 항균효과", 한국식품영양과학회지, 36(4), pp.470~475 (2007), 한국식품영양과학회
- 이종기, "황금탕 및 황금추출물의 식중독 세균들에 대한 생육억제 효과", 한국식품영양과학회지, 34(10), pp.1606~1610 (2005), 한국식품영양과학회
- Hong ND, "Studies on the efficacy of combined preparation of crude drugs", Kor J Pharmacogn, 13(1), pp.26~32 (1982)
- 장재선, "항생제 내성균에 대한 황금 추출물의 항균효과", 한국식품영양학회지, 24(4), pp.708~712 (2011), 한국식품영양학회
- 단체급식에서의 HACCP 도입방안에관한 연구, "유화춘", 한국보건산업진흥원, ~ (1999)
- Alterkruse SF, "Emerrging foodborne disease", Emerg Infedt Dis, 3, pp.285~293 (1997)
- 항생제내성균주은행, "항생제내성균 검사 표준 시험법", 식품의약품안정청
- 이주영, "식품에서 분리한 황색포도상구균의 항생제 내성 특징 및 균막 형성", 한국미생물·생명공학회지, 41(1), pp.96~104 (2013), 한국미생물·생명공학회[DOI]
- 윤은정, "시프로플록사신 내성 황색 포도상 구균에서 MIC의 하향 분포로 입증된 DW-224a의 in vitro 항균 활성의 비교 우수성", Journal of Pharmaceutical Investigation, 39(6), pp.431~435 (2009), 한국약제학회
- 정경해, "지역사회병원에서의 황색포도상구균혈증에 대한 임상적 고찰", 감염과화학요법, 29(1), pp.39~47 (1997)
- 강성구, "인체생물학", pp.363~367 (2006), 아카데미서적
- 신민교, "임상본초학", pp.372~400 (2000), 영림사
- 최용태, "침구학", pp.198~199 (1988), 집문당
- 한종현, "한방약리학", pp.187~190 (2004), 의성당
- 최미숙, "황금, 황련, 황백 (Scutellaria baicalensis, Coptis chinensis, Phellodendron amurense) 추출물의 항균효과", 석사, 뷰티산업학, pp.2010
- 서형식, "황련의 증량에 따른 전탕액이 Staphylococcus aureus에 미치는 항균효과에 대한 실험정 연구", Journal of Pharmacopuncture, 10(3), pp.47~52 (2007), 대한약침학회