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透膿散 및 瓜蔞牛蒡湯의 Staphylococcus aureus에 대한 In Vitro 항균력 평가

In Vitro Antibacterial Effects of Tunong-san and Kwaruwoobang-tang Aqueous Extracts against Staphylococcus aureus

김동철|박영선|장세란
대한한방부인과학회 (The Society Of Oriental Obstetrics & Gynecology)|2012.08|대한한방부인과학회지 (The Journal of Oriental Obstetrics ＆ Gynecology)|제25권 제3호|pp. 27-39|DOI http://dx.doi.org/

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Staphylococcus aureusCiprofloxacinAntibacterial EffectsMastitis Tunong-sanKwaruwoobang-tang
주제분류한의학

초록

Objectives: Recently Ciprofloxacin, used in the treatment of mastitis, showed many serious side effects. The object of this study was to recognize whether TNS and KWT can be used in the treatment of mastitis by observing the in vitro antibacterial effects of TNS and KWT aqueous herbal extracts against S. aureus. Methods: Antibacterial activities of TNS and KWT aqueous extracts against S. aureus ATCC 25923 were detected using standard agar microdilution methods. In addition, the effects on the bacterial growth curve were monitored at MIC and MIC×2 levels. The effects on the intracellular killing and bacterial invasion of individual test materials were also observed using Raw 264.6 and MCF-7. The results were compared with Ciprofloxacin, a second generation of quinolone antibiotics in the present study. Results: MIC of aqueous extracts of TNS and KWT against S. aureus were detected as (0.313±0.107) and (0.137±0.053) ㎎/㎖, respectively. MIC of Ciprofloxacin was detected as (0.469±0.297) ㎍/㎖ at same conditions. In addition, TNS, KWT aqueous herbal extracts and Ciprofloxacin were also showed marked dosage-dependent inhibition of bacterial growth, and dramatical inhibitions on the both intracellular killing assays and bacterial invasion using Raw 264.6 and MCF-7 cells were detected. Conclusions: The results obtained in this study suggest that TNS and KWT aqueous herbal extracts showed antibacterial effects against S. aureus, and they also showed dosage-dependent inhibitory effects on the bacterial growth. And they showed the significant intracellular killing and bacterial invasion effects. It means,KWT and TNS may show more potent anti-infectious effects against S. aureus in vivo.

Abstract

Objectives: Recently Ciprofloxacin, used in the treatment of mastitis, showed many serious side effects. The object of this study was to recognize whether TNS and KWT can be used in the treatment of mastitis by observing the in vitro antibacterial effects of TNS and KWT aqueous herbal extracts against S. aureus. Methods: Antibacterial activities of TNS and KWT aqueous extracts against S. aureus ATCC 25923 were detected using standard agar microdilution methods. In addition, the effects on the bacterial growth curve were monitored at MIC and MIC×2 levels. The effects on the intracellular killing and bacterial invasion of individual test materials were also observed using Raw 264.6 and MCF-7. The results were compared with Ciprofloxacin, a second generation of quinolone antibiotics in the present study. Results: MIC of aqueous extracts of TNS and KWT against S. aureus were detected as (0.313±0.107) and (0.137±0.053) ㎎/㎖, respectively. MIC of Ciprofloxacin was detected as (0.469±0.297) ㎍/㎖ at same conditions. In addition, TNS, KWT aqueous herbal extracts and Ciprofloxacin were also showed marked dosage-dependent inhibition of bacterial growth, and dramatical inhibitions on the both intracellular killing assays and bacterial invasion using Raw 264.6 and MCF-7 cells were detected. Conclusions: The results obtained in this study suggest that TNS and KWT aqueous herbal extracts showed antibacterial effects against S. aureus, and they also showed dosage-dependent inhibitory effects on the bacterial growth. And they showed the significant intracellular killing and bacterial invasion effects. It means,KWT and TNS may show more potent anti-infectious effects against S. aureus in vivo.

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