Condensed Molasses Solubles (CMS)/당밀 혼합물이 반추위 미생물 단백질 합성량에 미치는 영향
The Effects of Condensed Molasses solubles (CMS)/Molasses Mixtures on Ruminal Microbial protein synthesis
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본 연구는 monosodium glutamate의 생산 후 발생되는 부산물인 condensed molasses solubles(CMS)가 반추가축의 질소 공급원으로서 반추위 미생물 단백질 합성에 미치는 효과를 조사하기 위하여 수행하였다. 반추위 canulae가 부착된 4마리의 비착유소를 4 x 4 라틴 방각법에 적용하여 실험을 수행하였으며, 4개의 처리구는 다음과 같다 : (1) 기초사료(앞착된 보리 3kg/일과 보리짚의 자유채식), (2) 기초사료에 당밀 200g/일 그리고 물 300g/일 첨가, (3) 기초사료에 당밀 200g/일, CMS 100g/일 그리고 물 200g/일 첨가 (4) 기초사료에 당밀 200g/일, CMS 200g/일, 그리고 물 100g/일 첨가. CMS의 첨가수준에 따른 반추위내 발효양상은 처리구간에 유의적인 차이가 없었으나, CMS를 200g/일을 첨가한 처리구가 다른 처리구들에 비해 반추위내 미생물 단백질 합성량의 표시로서 이용된 allantoin/creatinine의 비율을 증가시키는 경향이 나타났다 (P<0.10).
Abstract
An experiment was conducted to evaluate condensed molasses solubles(CMS, a by-product from monosodium glutamate production) as a source of nitrogen for ruminant with particular reference to its effects on microbial protein synthesis. Four non-lactating dairy cows fitted with rumen cannulas were used in a 4 x 4 Latin square with 14-day periods. The four treatments were (1) basal diet consisting of barley straw ad libitum and 3 kg/d of rolled barley, (2) basal diet plus 200 g/d molasses and 300 g/d water, (3) basal diet plus 200 g/d molasses, 100 g/d CMS and 200 g/d water, (4) basal diet plus 200 g/d molasses, 200 g/d CMS and 100 g/d water. Ruminal pH remained at high levels and showed little variation during the day between treatments. The concentration of total and individual VFA in the rumen was similar between treatments. There was no difference in the concentration of ammonia in the rumen between treatments, although the intake of nitrogen in molasses/CMS mixture treatments was higher than that of control and molasses treatment. But there was a suggestion of an increased synthesis of microbial protein with the higher level of inclusion of CMS when the allantoin/creatinine ratio was used as an index of microbial protein production (P<0.10).
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