목초 사일리지 급여 시 보리와 채종박 보충급여에 의한 제 3위 소화액내 Soluble Non-ammonia Nitrogen Fraction의 Flow 패턴 변화
Diurnal Patterns in the Flow of Escapable Soluble Non-Ammonia Nitrogen Fractions in Omasal Digesta as Influenced by Barley and Rapeseed Meal Supplementation in Cows Fed Grass Silage Based Diet
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참고문헌
초록
본 연구는 목초 사일리지를 기초사료로 급여한 젖소에 있어서 보리와 유채박 급여가 제 3위 소화액 내 soluble non-ammonia nitrogen fractions(SNAN fractions; 아미노산, 펩티드, 용해성 단백질 및 total SNAN)의 flow 패턴변화를 측정하기 위하여 실시하였다. 반추위 캐뉼라가 장착된 4마리 젖소는 4×4 라틴방각법에 의해 기초사료로 grass-red clover silage를 자유급여하여 사일리지 단독급여구(GS), 기초사료 + 보리6.0kg/d 급여구, 기초사료 + 유채박2.1kg/d 급여구 및 기초사료 + 보리6.0kg/d + 유채박2.1kg/d 급여구로 배치하였다. 제 3위 소화액은 제 3위 소화액 채취기구를 이용하여 사료급여 후 1.5h 간격으로 채취하였고, 그 소화액 내 SNAN fractions 정량은 ninhydrin assay를 이용하여 분석하였다. 보충사료 급여는 GS구에 비해 비록 통계학적 유의성은 없었지만 total SNAN flow를 증가시켰다. 제 3위 소화액 내 펩티드 flow 패턴은 12시간 feeding cycle 내내 SNAN fractions 중 양적으로 가장 높았으며, GS구에 비해 보충사료급여구에서 높은 펩티드 flow 패턴을 보였다. GS구를 제외한 보충사료처리구간에는 SNAN fractions flow 패턴 차이가 없었다. 15N을 이용하여 분석된 사료유래 SNAN fractions flow 패턴에서는 보충사료급여구가 GS구에 비해 높게 나타났다. 이상의 결과 제 3위로 유입되는 펩티드 flow는 전체 SNAN flow 중 양적으로 가장 중요한 N 이며, 보충사료 급여 시 그 펩티드 flow의 증가 가능성을 시사한다.
Abstract
The present study was conducted to measure diurnal patterns in the flow of soluble non-ammonia nitrogen (SNAN) fractions in the liquid phase of digesta entering the omasum of cows fed grass-red clover silage supplemented with barley and rapeseed meal. Four ruminally cannulated cows were fed, in a 4×4 Latin square design, grass-red clover silage alone (GS) or supplemented with (on a DM basis) 6.0kg/d of barley grain, 2.1kg/d of rapeseed meal or 6.0kg/d of barley and 2.1kg/d rapeseed meal. Omasal digesta was taken using an omasal sampling system at 1.5h intervals during a 12h feeding cycle, and SNAN fractions (free AA, peptide and soluble protein) in the omasal digesta were assessed using ninhydrin assay. Dietary supplementation numerically increased the mean flow of SNAN fractions relative to GS diet despite the lack of statistical significance. Diurnal patterns in the flow of peptide entering the omasum during a 12h feeding cycle appeared to be highest immediately after feeding, declined by 10.0h post-feeding and slightly increased thereafter. In SNAN fractions, the flow of peptide was higher for supplemented diets than for GS diet throughout the feeding cycle. Based on the microbial contribution to total SNAN using 15N, diurnal patterns in the flow of dietary SNAN for dietary supplemented diets appeared to be higher compared with GS diets. Present results may conclude that peptide flow is quantitatively the most important N in SNAN fractions and that dietary supplementation can increase peptide flow entering the omasal canal.
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