강황 발효액이 고지방 섭취 흰쥐의 비만에 미치는 효과
Effects of Fermented Turmeric Extracts on the Obesity in Rats Fed a High-Fat Diet
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본 실험에서는 발효 강황 발효액의 급여가 내장지방과 혈청지질에 미치는 효과를 살펴보고자 수행하였다. 강황과 흑설탕을 동일 비율로 혼합하여 12개월간 숙성 발효하였다. 발효액은 HPLC를 이용해 발효 후 유리당의 함량을 분석하였다. 동물실험은 총 3군으로 일반 대조군에는 고지방사료와 흑설탕 7.2%(HFD), 처리군에는 고지방 사료에 흑설탕 7.2%와 강황 12.8%(TP), 고지방 사료에 분말화한 발효 강황 20%(FTP)을 첨가한 배합사료를 4주간 급여하였다. 혈청지질에서 총 콜레스테롤과 LDL-콜레스테롤은 HFD군에 비하여 모두 감소하는 경향이었다. 혈청 중 아디포넥틴과 렙틴은 HFD군에 비해서 FTP군이 유의적으로 낮았으며(P<0.05), TNF-α은 HFD군에 비해서 FTP군이 감소하였다. 또한 간 손상 지표인 혈청의 AST는 HFD군에 비해서 FTP군이 감소하였다. 이를 통해 발효 강황 발효액은 발효 전보다 발효 후에 고지방식이를 투여한 흰쥐에 있어서 비만을 억제할 수 있는 가능성이 있는 것으로 사료되었다.
Abstract
This study was designed to investigate the effects of fermented turmeric extract on body weight, abdominal fat mass and biochemical markers related to obesity in rats fed high fat diet. Turmeric and brown sugar was equally mixed and fermented for one year. Wistar rats(weighing about 125g(15 rats)) were divided into three groups: high fat diet(20.9%) and brown sugar 7.2% (HFD), turmeric powder in replace of corn starch 12.8%, brown sugar 7.2% (TP), fermented turmeric powder 20%(FTP) for four weeks. The final body weight was about 425g. The retroperitoneal fat weights in TP group showed the tendency to decrease regardless of feed intake in the rats. Serum levels of total cholesterol and LDL-cholesterol in the FTP group showed the lowering tendency than those of the HFD group(p<0.05). Serum levels of leptin, adiponectin and aspartate aminotransferase(AST) in the FTP group was significantly(p<0.05) lower than that of the HFD group, and serum level of tumor necrosis factor-α(TNF-α) in all the groups were similar. These results suggest that fermented turmeric extract might be effective to prevent obesity in rats fed high fat diet.
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