Nutrient requirements in Hanwoo cows with artificial insemination: effects on blood metabolites and embryo recovery rate
2
조회
0
다운로드
2
인용
0
참고문헌
키워드
초록
Here, we investigated the effects of different nutrient requirements (NR) on blood metabolites, transferable embryo number after multiple superovulations with artificial insemination (AI), body condition score (BCS), and estrus cycle in Hanwoo cow. Nineteen Hanwoo cows were randomly divided into three groups (80%, 100%, and 120% NR, containing 6, 8, and 5 individuals, respectively) and fed based on the NR. In experiment 1, glucose, total cholesterol, triglyceride, blood urea nitrogen (BUN), aspartate aminotransferase (AST), alanine aminotransferase (ALT), non-esterified fatty acids (NEFA), albumin (ALB), and total protein (TP) were analyzed. In experiment 2, total number of recovered embryos and transferable embryos was examined after embryo recovery and multiple superovulations with AI. In experiment 3, body weight, BCS, and estrus cycle were examined. In experiment 1, total cholesterol was significantly different among the 80%, 100%, and 120% NR groups (126.5 ± 3.3, 152.6 ± 2.4, and 177.4 ± 1.8 mg/dL, respectively, p < 0.05). The triglyceride and BUN levels in the 120% NR group were significantly higher than those in the 80% and 100% groups (p < 0.05). The NEFA levels were significantly different among the 80%, 100%, and 120% NR groups (440.5 ± 18.2, 318.5 ± 23.1, and 195.1 ± 8.5 ЧEq/L, respectively, p < 0.05). The AST and TP levels in the 80% NR group were significantly lower than those in the 100% and 120% NR groups (p < 0.05). In experiment 2, the 120% NR group showed a higher percentage of transferable embryos than the 80% and 100% groups (p < 0.01). The mean body weight and BCS among the 80%, 100%, and 120% NR groups were significantly different (p < 0.05). The estrus cycle in the 80% NR group was delayed compared with the 100% and 120% NR groups (20.8 ± 0.2 and 21.2 ± 0.5 days, respectively). In conclusion, the blood metabolic tests proved that Hanwoo cows with 120% NR can produce a large number of transferable embryos. Thus, 120% NR is the appropriate feeding level for this type of cows as it results in the production of a large number of transferable embryos by multiple superovulations with AI.
Abstract
Here, we investigated the effects of different nutrient requirements (NR) on blood metabolites, transferable embryo number after multiple superovulations with artificial insemination (AI), body condition score (BCS), and estrus cycle in Hanwoo cow. Nineteen Hanwoo cows were randomly divided into three groups (80%, 100%, and 120% NR, containing 6, 8, and 5 individuals, respectively) and fed based on the NR. In experiment 1, glucose, total cholesterol, triglyceride, blood urea nitrogen (BUN), aspartate aminotransferase (AST), alanine aminotransferase (ALT), non-esterified fatty acids (NEFA), albumin (ALB), and total protein (TP) were analyzed. In experiment 2, total number of recovered embryos and transferable embryos was examined after embryo recovery and multiple superovulations with AI. In experiment 3, body weight, BCS, and estrus cycle were examined. In experiment 1, total cholesterol was significantly different among the 80%, 100%, and 120% NR groups (126.5 ± 3.3, 152.6 ± 2.4, and 177.4 ± 1.8 mg/dL, respectively, p < 0.05). The triglyceride and BUN levels in the 120% NR group were significantly higher than those in the 80% and 100% groups (p < 0.05). The NEFA levels were significantly different among the 80%, 100%, and 120% NR groups (440.5 ± 18.2, 318.5 ± 23.1, and 195.1 ± 8.5 ЧEq/L, respectively, p < 0.05). The AST and TP levels in the 80% NR group were significantly lower than those in the 100% and 120% NR groups (p < 0.05). In experiment 2, the 120% NR group showed a higher percentage of transferable embryos than the 80% and 100% groups (p < 0.01). The mean body weight and BCS among the 80%, 100%, and 120% NR groups were significantly different (p < 0.05). The estrus cycle in the 80% NR group was delayed compared with the 100% and 120% NR groups (20.8 ± 0.2 and 21.2 ± 0.5 days, respectively). In conclusion, the blood metabolic tests proved that Hanwoo cows with 120% NR can produce a large number of transferable embryos. Thus, 120% NR is the appropriate feeding level for this type of cows as it results in the production of a large number of transferable embryos by multiple superovulations with AI.
참고문헌 (43)
- Villagodoy A, "Influence of energy-balance and body condition on estrus and estrous cycles in Holstein heifers", J Dairy Sci, 73, pp.2759~2765 (1990)
- Kruse SG, "Influence of post-insem ination nutrition on embryonic development in beef heifers", Theriogenology, 90, pp.185~190 (2017)
- Butler WR, "Energy balance relationships with follicular development, ovulation and fertility in postpartum dairy cows", Livest Prod Sci, 83, pp.211~218 (2003)
- Edmonson AJ, "A body condition scoring chart for Holstein dairy-cows", J Dairy Sci, 72, pp.68~78 (1989)
- Montiel F, "Body condition and suckling as factors influencing the duration of postpartum anestrus in cattle : a review", Anim Reprod Sci, 85, pp.1~26 (2005)
- Makarevich AV, "Quality of preimplantation embryos recovered in vivo from dairy cows in relation to their body condition", Zygote, 24, pp.378~388 (2016)
- Garnsworthy PC, "Nutrition, metabolism, and fertility in dairy cows: 1. dietary energy source and ovarian function", J Dairy Sci, 91, pp.3814~3823 (2008)
- Cozzi G, "Reference values for blood parameters in Holstein dairy cows : effects of parity, stage of lactation, and season of production", J Dairy Sci, 94, pp.3895~3901 (2011)
- Cetica P, "Activity of key enzymes involved in glucose and triglyceride catabolism during bovine oocyte maturation in vitro", Reproduction, 124, pp.675~681 (2002)
- Leroy JL, "The in vitro development of bovine oocytes after maturation in glucose and β-hydroxybutyrate concentrations associated with negative energy balance in dairy cows", Reprod Domest Anim, 41, pp.119~123 (2006)
- Rabiee AR, "Relationships among metabolites influencing ovarian function in the dairy cow", J Dairy Sci, 82, pp.39~44 (1999)
- Felix Diaz González, "Relationship among blood indicators of lipomobilization and hepatic function during early lactation in high-yielding dairy cows", Journal of Veterinary Science, 12(3), pp.251~255 (2011), 대한수의학회
- Bobe G, "Pathology, etiology, prevention, and treatment of fatty liver in dairy cows", J Dairy Sci, 87, pp.3105~3124 (2004)
- Rhoads ML, "Detrimental effects of high plasma urea nitrogen levels on viability of embryos from lactating dairy cows", Anim Reprod Sci, 91, pp.1~10 (2006)
- Santos P, "Effects of plasma urea nitrogen levels on the bovine oocyte ability to develop after in vitro fertilization", Reprod Domest Anim, 44, pp.783~787 (2009)
- Smuts MP, "Serum albumin concentration of donor cows as an indicator of developmental competence of oocytes", Theriogenology, 125, pp.184~192 (2019)
- Chapinal N, "The association of serum metabolites in the transition period with milk production and early-lactation reproductive performance", J Dairy Sci, 95, pp.1301~1309 (2012)
- Lomander H, "Test accuracy of metabolic indicators in predicting decreased fertility in dairy cows", J Dairy Sci, 95, pp.7086~7096 (2012)
- Jorritsma R, "Effects of non-esterified fatty acids on bovine granulosa cells and developmental potential of oocytes in vitro", Anim Reprod Sci, 81, pp.225~235 (2004)
- Mu YM, "Saturated FFAs, palmitic acid and stearic acid, induce apoptosis in human granulosa cells", Endocrinology, 142, pp.3590~3597 (2001)
- Callesen H, "Practical aspects of multiple ovulation embryo transfer in cattle", Anim Reprod Sci, 42, pp.215~226 (1996)
- Colazo MG, "Evaluation of a modified GnRH-based timed-AI protocol associated with estrus detection in beef heifers inseminated with sex-selected or conventional semen", Theriogenology, 118, pp.90~95 (2018)
- Stringfellow DA, "Manual of the international embryo transfer society: a procedural guide and general information for the use of embryo transfer technology, emphasizing sanitary precautions", International Embryo Transfer Society, ~ (1998)
- Santos JEP, "Nutritional management of the donor cow", Theriogenology, 69, pp.88~97 (2008)
- Yaakub H, "Effect of type and quantity of concentrates on superovulation and embryo yield in beef heifers", Theriogenology, 51, pp.1259~1266 (1999)
- Sales JNS, "Effects of a high-energy diet on oocyte quality and in vitro embryo production in Bos indicus and Bos taurus cows", J Dairy Sci, 98, pp.3086~3099 (2015)
- Mollo MR, "Embryo production in superovulated Nelore heifers under low or high feed take", Acta Sci Vet, 35, pp.s1241~ (2007)
- Bastos MR, "Effect of nutritional flushing on the superovulatory response of crossbred cows", Acta Sci Vet, 35, pp.s1242~ (2007)
- Mollo MR, "Embryo production in heifers with low or high dry matter intake submitted to superovulation", Theriogenology, 92, pp.30~35 (2017)
- Uhde K, "Exposure to elevated glucose concentrations alters the metabolomic profile of bovine blastocysts", PLOS ONE, 13, pp.e0199310~ (2018)
- Bermejo-Alvarez P, "Effect of glucose concentration during in vitro culture of mouse embryos on development to blastocyst, success of embryo transfer, and litter sex ratio", Mol Reprod Dev, 79, pp.329~336 (2012)
- Orsi NM, "Amino acid metabolism of preimplantation bovine embryos cultured with bovine serum albumin or polyvinyl alcohol", Theriogenology, 61, pp.561~572 (2004)
- Leroy JLMR, "Comparison of embryo quality in high-yielding dairy cows, in dairy heifers and in beef cows", Theriogenology, 64, pp.2022~2036 (2005)
- Cetica P, "Activity of key enzymes involved in glucose and triglyceride catabolism during bovine oocyte maturation in vitro", Reproduction, 124, pp.675~681 (2002)
- 강성식, "한우의 번식률 개선을 위한 영양대사물질 기초분석", 한국초지조사료학회지, 38(4), pp.331~336 (2018), 한국초지조사료학회[DOI]
- 손준규, "한우 공란우 과배란 처리와 혈액 내 요소태 질소 수준과의 관계", Reproductive & Developmental biology, 34(3), pp.201~205 (2010), 사단법인 한국동물생명공학회
- Wathes DC, "Influence of negative energy balance on cyclicity and fertility in the high producing dairy cow", Theriogenology, 68, pp.S232~S241 (2007)
- Van Hoeck V, "Elevated non-esterified fatty acid concentrations during bovine oocyte maturation compromise early embryo physiology", PLOS ONE, 6, pp.e23183~ (2011)
- Du X, "Elevated apoptosis in the liver of dairy cows with ketosis", Cell Physiol Biochem, 43, pp.568~578 (2017)
- Kalaitzakis E, "Clinicopathological evaluation of downer dairy cows with fatty liver", Can Vet J, 51, pp.615~622 (2010)
- Kadokawa H, "A field study on the relationship between body condition and embryo production in superovulated Holstein yearling heifers", J Dairy Sci, 91, pp.1087~1091 (2008)
- Siddiqui MAR, "Effect of feeding and body condition score on multiple ovulation and embryo production in zebu cows", Reprod Domest Anim, 37, pp.37~41 (2002)
- Butler WR, "Interrelationships between energy-balance and postpartum reproductive function in dairy-cattle", J Dairy Sci, 72, pp.767~783 (1989)